FlyBase Allele Report: Dmel\Atg12[RNAi.UAS]

General Information

Symbol

Dmel\Atg12^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0176393

Feature type

allele

Associated gene

Dmel\Atg12

Associated Insertion(s)

Carried in Construct

P{UAS-Atg12.RNAi}

Also Known As

UAS-Atg12^RNAi, UAS-Atg12-IR, Atg12-RNAi, Atg12^RNAi

Key Links

Transgenic product class

RNAi_reagent

(Scott et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Scott et al., 2004)

Mutagen

in vitro construct

(Scott et al., 2004, Scott et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Atg12.RNAi}

Cytology

Description

Inverted repeat construct, with 3'-5'.5'-3' inverted repeats whose expression is driven by UASt.

(Scott et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Atg12

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Kennedy's disease

modeled by Hsap\AR^Q52.UAS

(Pandey et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | RU486 conditional, with Scer\GAL4^{Act5C.Switch.PR}

(Ren et al., 2009)

abnormal locomotor behavior, with Scer\GAL4^Toll-6-D42

(Batlevi et al., 2010)

increased cell death | chemical conditional, with Scer\GAL4^Act5C.PU

(Tang et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Atg12^{dsRNA.Scer\UAS} under the control of either Scer\GAL4^esg-NP5130 or Scer\GAL4^{Myo31DF-NP0001} results in increased intestinal stem cell proliferation in response to paraquat or Ecc15. A dramatic increase in cell death (cleaved caspase-3 levels) is observed in response to paraquat when Atg12^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^Act5C.PU.

(Tang et al., 2013)

Clonal expression of Atg12^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PP appears to block the formation of autophagy-related vesicles in larval fat bodies under starvation conditions compared with wild-type.

(Tang et al., 2011)

Expression of Atg12^{dsRNA.Scer\UAS} in the motor neurons of third instar larvae under the control of Scer\GAL4^D42, or in muscles under the control of Scer\GAL4^C57, results in larval motility defects.

(Batlevi et al., 2010)

Animals expressing Atg12^{dsRNA.Scer\UAS} in the salivary glands under the control of Scer\GAL4^fkh.PH display persistent salivary gland cell fragments in 81% of pupae.

(McPhee et al., 2010)

Flies expressing Atg12^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{Act5C.Switch.PR} in the presence of RU486 show reduced lysotracker red staining of the adult midgut compared to controls in both fed and fasting flies.

Adult expression of Atg12^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{Act5C.Switch.PR} in the presence of RU486 has no effect on adult lifespan.

The lifespan of flies injected with E.coli is significantly reduced by expression of Atg12^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{Act5C.Switch.PR} in the presence of RU486. These flies show a significant increase in bacterial load compared to control flies.

(Ren et al., 2009)

When expression is driven in the fat body results in a substantial decrease in the nuimber of autolysisones.

(Scott et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg12^RNAi.UAS, Scer\GAL4^Act5C.PU has increased cell death | chemical conditional phenotype, suppressible by Atg9^GD2029, Scer\GAL4^Act5C.PU

(Tang et al., 2013)

Enhancer of

Statement

Reference

Atg12^RNAi.UAS, Scer\GAL4^GMR.PF is an enhancer of visible | heat sensitive phenotype of Prosβ2^1.UAS, Scer\GAL4^GMR.PF

(Pandey et al., 2007)

Atg12^RNAi.UAS, Scer\GAL4^GMR.PF is an enhancer of visible | drug conditional phenotype of Hsap\AR^Q52.UAS, Scer\GAL4^GMR.PF

(Pandey et al., 2007)

NOT Enhancer of

Statement

Reference

Scer\GAL4^{Act5C.Switch.PR}/Atg12^RNAi.UAS is a non-enhancer of long lived phenotype of chico¹

(Bjedov et al., 2020)

Atg12^RNAi.UAS, Scer\GAL4^GMR.long is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.long, Zzzz\CGG^90.UAS.EGFP

(Oh et al., 2015)

Atg12^RNAi.UAS, Scer\GAL4^GMR.PF is a non-enhancer of visible | heat sensitive phenotype of HDAC6^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Todd et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^Act5C.PU/Atg12^RNAi.UAS is a suppressor of chemical resistant | dominant phenotype of puc^B48

(Tang et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{Act5C.Switch.PR}/Atg12^RNAi.UAS is a non-suppressor of long lived phenotype of chico¹

(Bjedov et al., 2020)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Atg12^RNAi.UAS, Scer\GAL4^fkh.PH has embryonic/larval salivary gland phenotype, non-enhanceable by drpr^RNAi.I.UAS, Scer\GAL4^fkh.PH

(McPhee et al., 2010)

Suppressed by

Statement

Reference

Atg12^RNAi.UAS, Scer\GAL4^Act5C.PP has fat body | somatic clone | calorie restriction conditional phenotype, suppressible by sqh^{T20A.S21A.UAS}, Scer\GAL4^Act5C.PP

(Tang et al., 2011)

Atg12^RNAi.UAS, Scer\GAL4^Act5C.PP has autophagosome | somatic clone | calorie restriction conditional phenotype, suppressible by sqh^{T20A.S21A.UAS}, Scer\GAL4^Act5C.PP

(Tang et al., 2011)

Atg12^RNAi.UAS, Scer\GAL4^Act5C.PP has fat body | somatic clone | calorie restriction conditional phenotype, suppressible by sqa^T279A.UAS, Scer\GAL4^Act5C.PP

(Tang et al., 2011)

Atg12^RNAi.UAS, Scer\GAL4^Act5C.PP has autophagosome | somatic clone | calorie restriction conditional phenotype, suppressible by sqa^T279A.UAS, Scer\GAL4^Act5C.PP

(Tang et al., 2011)

Atg12^RNAi.UAS, Scer\GAL4^Act5C.PP has fat body | somatic clone | calorie restriction conditional phenotype, suppressible by sqa^RNAi.UAS, Scer\GAL4^Act5C.PP

(Tang et al., 2011)

Atg12^RNAi.UAS, Scer\GAL4^Act5C.PP has autophagosome | somatic clone | calorie restriction conditional phenotype, suppressible by sqa^RNAi.UAS, Scer\GAL4^Act5C.PP

(Tang et al., 2011)

Enhancer of

Statement

Reference

Atg12^RNAi.UAS, Scer\GAL4^GMR.PF is an enhancer of eye | heat sensitive phenotype of Prosβ2^1.UAS, Scer\GAL4^GMR.PF

(Pandey et al., 2007)

Atg12^RNAi.UAS, Scer\GAL4^GMR.PF is an enhancer of eye | drug conditional phenotype of Hsap\AR^Q52.UAS, Scer\GAL4^GMR.PF

(Pandey et al., 2007)

NOT Enhancer of

Statement

Reference

Atg12^RNAi.UAS, Scer\GAL4^GMR.long is a non-enhancer of eye phenotype of Scer\GAL4^GMR.long, Zzzz\CGG^90.UAS.EGFP

(Oh et al., 2015)

Atg12^RNAi.UAS, Scer\GAL4^GMR.PF is a non-enhancer of eye | heat sensitive phenotype of HDAC6^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Todd et al., 2010)

Suppressor of

Statement

Reference

Atg12^RNAi.UAS, Scer\GAL4^fkh.PH is a suppressor of embryonic/larval salivary gland phenotype of Atg1^GS10797, Scer\GAL4^fkh.PH

(Berry and Baehrecke, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Atg12^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PU does not suppress the resistance to paraquat seen in puc^B48/+ mutant flies.

Expression of Atg9^GD2029 suppresses the increase in cell death (cleaved caspase-3 levels) observed in response to paraquat when Atg12^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^Act5C.PU.

(Tang et al., 2013)

Co-expression of sqh^{T20A.S21A.Scer\UAS} with Atg12^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PP strongly suppresses the Atg12^{dsRNA.Scer\UAS}-induced fat body phenotype.

Co-expression of sqa^{T279A.Scer\UAS} with Atg12^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PP significantly suppresses the Atg12^{dsRNA.Scer\UAS}-induced fat body phenotype.

Co-expression of sqa^{dsRNA.Scer\UAS} with Atg12^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PP significantly suppresses the Atg12^{dsRNA.Scer\UAS}-induced fat body phenotype.

(Tang et al., 2011)

Animals expressing Atg12^{dsRNA.Scer\UAS} and drpr^{dsRNA.I.Scer\UAS} in the salivary glands under the control of Scer\GAL4^fkh.PH display persistence of salivary gland cell fragments in 100% of pupae, and the phenotype is very similar to expression of Atg12^{dsRNA.Scer\UAS} alone. There is no increase in the amount of salivary gland material that persists, and multi-cell gland fragments are absent.

(McPhee et al., 2010)

Co-expression of Atg12^{dsRNA.Scer\UAS} suppresses the premature degradation of the salivary glands that is seen in animals expressing Atg1^GS10797 under the control of Scer\GAL4^fkh.PH.

(Berry and Baehrecke, 2007)

The rough eye phenotype seen in flies expressing Prosβ2^1.Scer\UAS under the control of Scer\GAL4^GMR.PF at 28^oC is enhanced by co-expression of Atg12^{dsRNA.Scer\UAS}.

The ability of HDAC6^{Scer\UAS.T:SV5\V5} to suppress the rough eye phenotype seen in flies expressing Prosβ2^1.Scer\UAS under the control of Scer\GAL4^GMR.PF at 28^oC is suppressed by co-expression of Atg12^{dsRNA.Scer\UAS}.

(Pandey et al., 2007)

Xenogenetic Interactions

Statement

Reference

The eye degeneration phenotype observed in adult flies expressing Zzzz\CGG^{90.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.long (and maintained at 28[o]C) is not modified by co-expression of Atg12^{dsRNA.Scer\UAS}.

(Oh et al., 2015)

Co-expression of Atg12^{dsRNA.Scer\UAS} has no effect on suppression of the Scer\GAL4^GMR.PF>Zzzz\CGG^{90.Scer\UAS.T:Avic\GFP-EGFP} phenotype by HDAC6^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}.

(Todd et al., 2010)

The rough eye phenotype seen in flies expressing Hsap\AR^Q52.Scer\UAS under the control of Scer\GAL4^GMR.PF in the presence of dihydrotestosterone (DHT) is enhanced by co-expression of Atg12^{dsRNA.Scer\UAS}.

The ability of HDAC6^{Scer\UAS.T:SV5\V5} to suppress the rough eye phenotype seen in flies expressing Hsap\AR^Q52.Scer\UAS under the control of Scer\GAL4^GMR.PF in the presence of dihydrotestosterone (DHT) is suppressed by co-expression of Atg12^{dsRNA.Scer\UAS}.

(Pandey et al., 2007)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Atg12^RNAi.UAS

Atg12^{dsRNA.Scer\UAS}

Atg12^dsRNA.UAS

atg12^KD

(Pandey et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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