FlyBase Allele Report: Dmel\Atg9[GD2029]

General Information

Symbol

Dmel\Atg9^GD2029

Species

D. melanogaster

Name

FlyBase ID

FBal0198791

Feature type

allele

Associated gene

Dmel\Atg9

Associated Insertion(s)

Carried in Construct

P{GD2029}

Also Known As

UAS-Atg9^RNAi, UAS-Atg9-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2029}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2029

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Yi et al., 2024)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates myotonic dystrophy type 1

modeled by Zzzz\CTG^i480.UAS.cGa

(Bargiela et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

short lived, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

short lived, with Scer\GAL4^tin.CΔ4

(Xu et al., 2019)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult glial cell, with Scer\GAL4^repo.PU

(Yi et al., 2024)

adult heart muscle cell | progressive, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

autophagosome | adult stage | decreased number, with Scer\GAL4^repo.PU

(Yi et al., 2024)

indirect flight muscle cell | day 40 of adulthood, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

intestinal stem cell | chemical conditional, with Scer\GAL4^NP0001

(Tang et al., 2013)

intestinal stem cell | chemical conditional, with Scer\GAL4^NP5130

(Tang et al., 2013)

mitochondrion | adult stage, with Scer\GAL4^Mef2.PU

(Xu et al., 2019)

omegasome | adult stage | decreased number, with Scer\GAL4^repo.PU

(Yi et al., 2024)

Detailed Description

Statement

Reference

The expression of Atg9^GD2029 under the control of Scer\GAL4^ap-md544 does not affect the curvature of the wing.

(Shinoda et al., 2019)

Flies expressing Atg9^GD2029 under the control of Scer\GAL4^Mef2.PU display progressive heart defects: thicker heart walls and a distorted lumen with a rough surface in older flies, increased systolic dimension throughout adult life, but no change in diastolic dimension and fractional shortening. Mitochondria in cardiomyocytes are abnormally elongated and narrowed, the mitochondrial network appears fused/enlarged and the mitochondrial volume density is significantly increased in 40-day old flies. Alongside, indirect flight muscles display fragmented mitochondria and reduced mitochondrial volume density. Neither males nor females display climbing defects.

(Xu et al., 2019)

Expression of Atg9^GD2029 under the control of either Scer\GAL4^esg-NP5130 of Scer\GAL4^{Myo31DF-NP0001} results in impaired intestinal stem cell proliferation in response to paraquat.

(Tang et al., 2013)

Flies expressing Atg9^GD2029 under the control of Scer\GAL4^GMR.PU show no defects in ommatidial structure.

(Chen et al., 2012)

Adults expressing Atg9^GD2029 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2029

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Atg9^GD2029, Scer\GAL4^ap-md544 is a suppressor | partially of visible | adult stage phenotype of Dcp-1^KK106134, Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Atg9^GD2029, Scer\GAL4^ap-md544 is a suppressor | partially of visible | adult stage phenotype of Decay^KK105137, Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Atg9^GD2029, Scer\GAL4^GMR.PU is a suppressor of abnormal size | adult stage phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Atg9^GD2029, Scer\GAL4^GMR.PU is a suppressor of visible | adult stage phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Atg9^GD2029, Scer\GAL4^hs.PU is a suppressor | heat sensitive | partially of abnormal size | adult stage | heat sensitive phenotype of Scer\GAL4^hs.PU, Zzzz\CTG^i480.UAS.cGa

(Bargiela et al., 2015)

Atg9^GD2029, Scer\GAL4^Act5C.PU is a suppressor of increased cell death | chemical conditional phenotype of Atg12^RNAi.UAS, Scer\GAL4^Act5C.PU

(Tang et al., 2013)

Scer\GAL4^Act5C.PU/Atg9^GD2029 is a suppressor of chemical resistant | dominant phenotype of puc^B48

(Tang et al., 2013)

NOT Suppressor of

Statement

Reference

Atg9^GD2029, Scer\GAL4^hs.PU is a non-suppressor of increased cell death | adult stage | heat sensitive phenotype of Scer\GAL4^hs.PU, Zzzz\CTG^i480.UAS.cGa

(Bargiela et al., 2015)

Other

Statement

Reference

Atg9^GD2029, Scer\GAL4^Act5C.PU, puc^B48 has chemical sensitive phenotype

(Tang et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

Atg9^GD2029, Scer\GAL4^ap-md544 is a suppressor | partially of wing phenotype of Dcp-1^KK106134, Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Atg9^GD2029, Scer\GAL4^ap-md544 is a suppressor | partially of wing phenotype of Decay^KK105137, Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Atg9^GD2029, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Atg9^GD2029, Scer\GAL4^GMR.PU is a suppressor of autophagosome | larval stage phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Atg9^GD2029, Scer\GAL4^GMR.PU is a suppressor of eye disc phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Atg9^GD2029, Scer\GAL4^hs.PU is a suppressor | heat sensitive | partially of adult somatic muscle cell of thorax | heat sensitive phenotype of Scer\GAL4^hs.PU, Zzzz\CTG^i480.UAS.cGa

(Bargiela et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Atg9^GD2029 under the control of Scer\GAL4^Act5C.PU suppresses the resistance to paraquat seen in puc^B48/+ mutant flies. Mortality rates are increased compared to controls.

Expression of Atg9^GD2029 suppresses the increase in cell death (cleaved caspase-3 levels) observed in response to paraquat when Atg12^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^Act5C.PU.

(Tang et al., 2013)

Xenogenetic Interactions

Statement

Reference

Co-expression of Atg9^GD2029 partially suppresses the decreased muscle area, but not the increased apoptosis seen in flies expressing Zzzz\CTG^{i480.Scer\UAS.cGa} under the control of Scer\GAL4^hs.PU in response to heat shock.

(Bargiela et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v10045

w¹¹¹⁸; P{GD2029}v10045

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2029

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atg9^GD10045

(Nagy et al., 2014)

Atg9^GD2029

Name Synonyms

Secondary FlyBase IDs

References (17)

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