FB2024_03 , released June 25, 2024
Allele: Dmel\parkHMS01651
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General Information
Symbol
Dmel\parkHMS01651
Species
D. melanogaster
Name
FlyBase ID
FBal0266558
Feature type
allele
Associated gene
Dmel\park
Associated Insertion(s)
Carried in Construct
P{TRiP.HMS01651}
Also Known As
Parkin RNAi
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Ni et al., 2011.7.19)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Ni et al., 2011.7.19)
Mutagen
in vitro construct
(Ni et al., 2011.7.19)
Progenitor genotype
Carried in construct
P{TRiP.HMS01651}
Cytology
Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dsRNA-HMS01651
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Machado-Joseph disease
      modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
      (Tsou et al., 2015)
      model of  cardiomyopathy
      is ameliorated by MarfRNAi.cUa.UAS
      (Bhandari et al., 2014)
      is ameliorated by Sod2UAS.cMa
      (Bhandari et al., 2014)
      is ameliorated by CG6878KK108566
      (Bhandari et al., 2014)
      is ameliorated by Sod1UAS.cWa
      (Bhandari et al., 2014)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adults expressing parkHMS01651 under the control of Scer\GAL4Act5C.PI or Scer\GAL4ple.PF show a progressive decline in climbing activity, and a progressive decrease in DA neuron number (PPL1, PPL2, PPM1/2 and PPM3 clusters); DA neurons also show decreased abundance of mitochondria.

      (Pirooznia et al., 2020)

      The expression of parkHMS01651 under the control of Scer\GAL4Act.PU in clones within mosaic midguts results in enterocytes during pupariation, but not after puparium formation, exhibiting a significant increase in the transverse cell area, a significant increase in the relative mitochondrial content and a significant decrease in the relative amount of autophagosomes, as assessed by a Atg8a fluorescent reporter, as compared to non-clone enterocytes.

      (Liu et al., 2016)

      Expression of parkHMS01651 under the control of Scer\GAL4tin.CΔ4 evokes heart tube hypocontractility at one and four weeks after eclosion, and increases cardiomyocyte mitochondrial size. The lifespan of these flies is similar to controls but the flies display an accelerated age-related decline in climbing performance. Heart tube end-systolic and end-diastolic dimensions are increased and mitochondria have decreased DNA, increase mitochondrial reactive oxygen species (ROS) and increased numbers of depolarized organelles.

      (Bhandari et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Scer\GAL4ple.PF, parkHMS01651 has dopaminergic neuron | adult stage | decreased number phenotype, suppressible by srlUAS.cRa/Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Scer\GAL4ple.PF, parkHMS01651 has mitochondrion | adult stage phenotype, suppressible by srlUAS.cRa/Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Scer\GAL4ple.PF, parkHMS01651 has dopaminergic neuron | adult stage | decreased number phenotype, suppressible by ParisGD9020, Scer\GAL4ple.PF

      (Pirooznia et al., 2020)

      Scer\GAL4tin.CΔ4, parkHMS01651 has mitochondrion phenotype, suppressible by Sod1UAS.cWa/Scer\GAL4tin.CΔ4

      (Bhandari et al., 2014)

      Scer\GAL4tin.CΔ4, parkHMS01651 has mitochondrion phenotype, suppressible by Sod2UAS.cMa/Scer\GAL4tin.CΔ4

      (Bhandari et al., 2014)

      Scer\GAL4tin.CΔ4, parkHMS01651 has mitochondrion phenotype, suppressible by MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4

      (Bhandari et al., 2014)

      Scer\GAL4tin.CΔ4, parkHMS01651 has adult heart phenotype, suppressible by Sod1UAS.cWa/Scer\GAL4tin.CΔ4

      (Bhandari et al., 2014)

      Scer\GAL4tin.CΔ4, parkHMS01651 has adult heart phenotype, suppressible by Sod2UAS.cMa/Scer\GAL4tin.CΔ4

      (Bhandari et al., 2014)

      Scer\GAL4tin.CΔ4, parkHMS01651 has adult heart phenotype, suppressible by MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4

      (Bhandari et al., 2014)

      Scer\GAL4tin.CΔ4, parkHMS01651 has cardial cell phenotype, suppressible by Sod1UAS.cWa/Scer\GAL4tin.CΔ4

      (Bhandari et al., 2014)

      Scer\GAL4tin.CΔ4, parkHMS01651 has cardial cell phenotype, suppressible by Sod2UAS.cMa/Scer\GAL4tin.CΔ4

      (Bhandari et al., 2014)

      Scer\GAL4tin.CΔ4, parkHMS01651 has cardial cell phenotype, suppressible by MarfRNAi.cUa.UAS, Scer\GAL4tin.CΔ4

      (Bhandari et al., 2014)
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of SodScer\UAS.cWa suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01651 is expressed under the control of Scer\GAL4tin.CΔ4.

      Expression of Sod2Scer\UAS.cMa suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01651 is expressed under the control of Scer\GAL4tin.CΔ4.

      Expression of CG6878KK108566 suppresses the heart tube hypocontraction and increase in mitochondrial reactive oxygen species (ROS) seen when parkHMS01651 is expressed under the control of Scer\GAL4tin.CΔ4.

      Co-expression of MarfmiRNA.cUa.Scer\UAS and parkHMS01651 under the control of Scer\GAL4tin.CΔ4 suppresses the heart tube cardiomyopathy and some of the mitochondrial defects seen when either transgene is expressed alone. The proportion of reactive oxygen species (ROS)-producing and depolarized organelles is reduced, and the reduction in mitochondrial DNA content usually seen in flies expressing parkHMS01651 is rescued. However, the mitochondria show characteristics of both parent lines: they are still enlarged as is seen in parkHMS01651, and appear fragmented as is seen with MarfmiRNA.cUa.Scer\UAS.

      (Bhandari et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of parkHMS01651 partially suppresses eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies. Co-expression of parkHMS01651 does not suppress the protective role of expression of Hsap\ATXN3Scer\UAS.WT.T:Zzzz\His6 in suppression of eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies.

      (Tsou et al., 2015)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      parkHMS01651
      Name Synonyms
      Secondary FlyBase IDs
        References (13)