The expression of myomiRNA.UAS under the control of either Scer\GAL4Mef2.PR, Scer\GAL4repo or Scer\GAL4Myo31DF-NP0001, but not Scer\GAL4C164, leads to an increase in the average larval weight, as compared to controls; however, there is no defect in developmental time to pupariation upon Scer\GAL4Mef2.PR-driven expression. The expression under the control of Scer\GAL4Mef2.PR, but not of Scer\GAL4repo, also leads to a significant increase in the size of larval muscles 6 and 7, as compared to controls.
The neurotransmission across the larval muscle 6 neuromuscular junction (NMJ) is increased upon the expression of myomiRNA.UAS under the control of Scer\GAL4Mef2.PR, as show by the significant increases in the amplitude of excitatory junctional currents (eEJC) and in the cumulative amplitude of miniature junctional currents (mEJC), as compared to controls; this expression also leads to significant increases in the density of presynaptic active zones (Brp-positive puncta) and in the length and branch number of the NMJ, as compared to controls. Expression under the control of Scer\GAL4repo, however, does not affect the neurotransmission across the larval NMJ in terms of eEJC amplitude and in mEJC cumulative amplitude, as compared to controls. Neurotransmission across the adult TTM neuron NMJ, but not the DLM neuron NMJ, is also affected by the expression of myomiRNA.UAS under the control of Scer\GAL4sli.PH, exhibiting a significant decrease in response latency upon electric stimulation of giant fibers, but not upon electric stimulation of the abdomen, as compared to controls.
Scer\GAL4Mef2.PR, myoRNAi.UAS has abnormal neurophysiology | larval stage phenotype, suppressible by sggKK108994/Scer\GAL4Mef2.PR
Scer\GAL4Mef2.PR, myoRNAi.UAS has abnormal neurophysiology | larval stage phenotype, suppressible by SmoxSDVD.UAS/Scer\GAL4Mef2.PR
Scer\GAL4Mef2.PR, myoRNAi.UAS has abnormal neurophysiology | larval stage phenotype, non-suppressible by MadSDVD.UAS, Scer\GAL4Mef2.PR
myoRNAi.UAS/Scer\GAL4Mef2.PR is a suppressor of abnormal neurophysiology | larval stage phenotype of Scer\GAL4Mef2.PR, sggKK108994
myoRNAi.UAS is a non-suppressor of lethal - all die before end of larval stage phenotype of AktRNAi.UAS.cUa, Scer\GAL4Mef2.PR
Scer\GAL4Mef2.PR, myoRNAi.UAS has larva phenotype, non-suppressible by SmoxSDVD.UAS/Scer\GAL4Mef2.PR
Scer\GAL4Mef2.PR, myoRNAi.UAS has larva phenotype, non-suppressible by MadSDVD.UAS, Scer\GAL4Mef2.PR