FlyBase Allele Report: Dmel\PolE1[NIG.6768R]

General Information

Symbol

Dmel\PolE1^NIG.6768R

Species

D. melanogaster

Name

FlyBase ID

FBal0267115

Feature type

allele

Associated gene

Dmel\PolE1

Associated Insertion(s)

Carried in Construct

P{NIG.6768R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21, Verma et al., 2012)

Progenitor genotype

Carried in construct

P{NIG.6768R}

Cytology

Description

UASt regulatory sequences drive expression of a 501bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-6768R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair | third instar larval stage

(Verma et al., 2012)

abnormal endomitotic cell cycle, with Scer\GAL4^l(1)BP-1

(Suyari et al., 2012)

abnormal mitotic cell cycle, with Scer\GAL4^ey.PU

(Suyari et al., 2012)

decreased occurrence of cell division | larval stage, with Scer\GAL4^ey.PU

(Suyari et al., 2012)

lethal - all die before end of pupal stage, with Scer\GAL4^Act5C.PU

(Suyari et al., 2012)

lethal - all die before end of pupal stage, with Scer\GAL4^Act.PU

(Suyari et al., 2012)

partially lethal - majority die | pupal stage

(Verma et al., 2012)

partially lethal - majority die | third instar larval stage

(Verma et al., 2012)

some die during pupal stage, with Scer\GAL4^Act5C.PU

(Suyari et al., 2012)

some die during pupal stage, with Scer\GAL4^Act.PU

(Suyari et al., 2012)

visible, with Scer\GAL4^ap-md544

(Suyari et al., 2012)

visible, with Scer\GAL4^ey.PU

(Suyari et al., 2012)

Phenotype Manifest In

embryonic/larval brain | third instar larval stage, with Scer\GAL4^Act5C.PU

(Suyari et al., 2012)

embryonic/larval salivary gland | third instar larval stage, with Scer\GAL4^Act5C.PU

(Suyari et al., 2012)

eye, with Scer\GAL4^ey.PU

(Suyari et al., 2012)

eye disc, with Scer\GAL4^ey.PU

(Suyari et al., 2012)

imaginal disc | third instar larval stage, with Scer\GAL4^Act5C.PU

(Suyari et al., 2012)

ommatidium, with Scer\GAL4^ey.PU

(Suyari et al., 2012)

salivary gland, with Scer\GAL4^l(1)BP-1

(Suyari et al., 2012)

wing, with Scer\GAL4^ap-md544

(Suyari et al., 2012)

Detailed Description

Statement

Reference

Expression of DNApol-ε^NIG.6768R under the control of either Scer\GAL4^GMR.PS, Scer\GAL4^en-e16E, Scer\GAL4^sd.PU or Scer\GAL4^Bx-MS1096 does not result in a detectable phenotype.

Expression of DNApol-ε^NIG.6768R under the control of Scer\GAL4^Act5C.PU results in a reduction in size of the salivary gland, brain lobes and imaginal discs compared to wild type.

Expression of DNApol-ε^NIG.6768R under the control of Scer\GAL4^ey.PU results in a small eye, with a reduction in the number of ommatidia. Incorporation of BrdU in the S phase zone posterior to the morphogenetic furrow is significantly reduced in eye imaginal discs in the animals.

Expression of DNApol-ε^NIG.6768R under the control of Scer\GAL4^ap-md544 results in an outstretched wing phenotype.

Expression of DNApol-ε^NIG.6768R under the control of Scer\GAL4^l(1)BP-1 results in a small salivary gland. The number and size of the salivary gland cell nuclei are decreased relative to wild type. The incorporation of BrdU in larvae 48 hours after hatching is severely decreased compared to wild type.

(Suyari et al., 2012)

Expression of DNApol-ε^NIG.6768R under the control of Scer\GAL4^Act5C.PI results in approximately 50% larval and pupal lethality.

Larvae expressing DNApol-ε^NIG.6768R under the control of Scer\GAL4^Act5C.PI show increased sensitivity to DNA damage in response to bleomycin compared to wild type.

(Verma et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

PolE1^NIG.6768R has partially lethal - majority die | third instar larval stage phenotype, enhanceable by l(3)pl10[+]/PolE1^pl10R

(Verma et al., 2012)

PolE1^NIG.6768R has partially lethal - majority die | pupal stage phenotype, enhanceable by l(3)pl10[+]/PolE1^pl10R

(Verma et al., 2012)

Other

Statement

Reference

PolA1^NIG.6349R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype

(Suyari et al., 2012)

PolD1^NIG.5949R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, RfC4^NIG.cUa, Scer\GAL4^ey.PU has visible phenotype

(Suyari et al., 2012)

Mcm10^NIG.9241R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has partially lethal - majority die phenotype

(Suyari et al., 2012)

Mcm10^NIG.9241R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, Psf2^NIG.18013R, Scer\GAL4^ey.PU has partially lethal - majority die phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, Psf2^NIG.18013R, Scer\GAL4^ey.PU has visible phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, PolH^NIG.7143R, Scer\GAL4^ey.PU has visible phenotype

(Suyari et al., 2012)

Iswi^NIG.8625R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has partially lethal - majority die phenotype

(Suyari et al., 2012)

Iswi^NIG.8625R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype

(Suyari et al., 2012)

PolA2^NIG.5923R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has visible phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, Prim2^NIG.5553R, Scer\GAL4^ey.PU has partially lethal - majority die phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, Prim2^NIG.5553R, Scer\GAL4^ey.PU has visible phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, Prim1^NIG.cUa, Scer\GAL4^ey.PU has visible phenotype

(Suyari et al., 2012)

Phenotype Manifest In

Other

Statement

Reference

PolA1^NIG.6349R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, RfC4^NIG.cUa, Scer\GAL4^ey.PU has adult head phenotype

(Suyari et al., 2012)

Mcm10^NIG.9241R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, Psf2^NIG.18013R, Scer\GAL4^ey.PU has adult head phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, PolH^NIG.7143R, Scer\GAL4^ey.PU has adult head phenotype

(Suyari et al., 2012)

PolA2^NIG.5923R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype

(Suyari et al., 2012)

Iswi^NIG.8625R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, Prim2^NIG.5553R, Scer\GAL4^ey.PU has adult head phenotype

(Suyari et al., 2012)

PolE1^NIG.6768R, Prim1^NIG.cUa, Scer\GAL4^ey.PU has adult head phenotype

(Suyari et al., 2012)

PolD1^NIG.5949R, PolE1^NIG.6768R, Scer\GAL4^ey.PU has adult head phenotype

(Suyari et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Flies co-expressing DNApol-ε^NIG.6768R and any one of DNApol-α180^NIG.6349R, DNApol-α73^NIG.5923R, DNApol-α60^NIG.5553R, DNApol-α50^NIG.cUa, DNApol-δ^NIG.5949R, RfC4^NIG.cUa, Mcm10^NIG.9241R, Psf2^NIG.18013R, DNApol-η^NIG.7143R or Iswi^NIG.8625R under the control of Scer\GAL4^ey.PU are headless.

(Suyari et al., 2012)

One copy of DNApol-ε^pl10R enhances the lethality seen when DNApol-ε^NIG.6768R is expressed under the control of Scer\GAL4^Act5C.PI, with only 23% of the expected progeny surviving to adulthood.

(Verma et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PolE1^NIG.6768R is rescued by PolE1^{Ct.UAS.Tag:HA}/Scer\GAL4^ey.PU

(Suyari et al., 2012)

Comments

Co-expression of DNApol-ε^{Ct.Scer\UAS.T:Ivir\HA1} rescues the small eye phenotype and mitotic cycle defects seen in the eye imaginal disc which are caused by expression of DNApol-ε^NIG.6768R under the control of Scer\GAL4^ey.PU.

Co-expression of DNApol-ε^{Ct.Scer\UAS.T:Ivir\HA1} does not rescue the endocycle defects seen in the salivary glands of animals expressing DNApol-ε^NIG.6768R under the control of Scer\GAL4^l(1)BP-1.

(Suyari et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DNApol-ε255^NIG.6768R

DNApol-ε^NIG.6768-R1

DNApol-ε^NIG.6768R

PolE1^NIG.6768R

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections