FB2024_03 , released June 25, 2024
Allele: Zzzz\CCG90.UAS.EGFP
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General Information
Symbol
Zzzz\CCG90.UAS.EGFP
Species
a. artificial
Name
FlyBase ID
FBal0280773
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Progenitor genotype
Carried in construct
Cytology
Description

UASt regulatory sequences are fused upstream of the EGFP version of Avic\GFP. A fragment containing 90 Zzzz\CCG repeats has been inserted between the transcriptional and translational start sites of Avic\GFP. The fragment containing the 90 Zzzz\CCG repeats is the same fragment as that contained within the P{UAS-CGG90-EGFP} construct, but it is inserted in reverse orientation in the P{UAS-CCG90-EGFP} construct.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PU results in a disorganised eye phenotype, with defects ranging from compressed ommatidia to disorganised bristles and fused ommatidia. Cross-sections of the eye show severe disruption and detachment of the retina and loss of photoreceptor cells.

Expression of Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Bx-MS1096 results in crumpled wings.

Expression of Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4C164 results in lethality in highly-expressing lines. Lower levels of expression result in flies that show a more rapid decline in their climbing ability with age than wild-type flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of both Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP and Zzzz\CGG90.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PU suppresses the disorganised external eye phenotype and photoreceptor cell degeneration which is seen when either construct is expressed singly under the control of Scer\GAL4GMR.PU. This mutual suppression by Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP and Zzzz\CGG90.Scer\UAS.T:Avic\GFP-EGFP does not occur if they are co-expressed under the control of Scer\GAL4GMR.PU in a AGO2414 homozygous background - in this case, the flies show pupal lethality and have a more severe eye phenotype than that seen when either construct is expressed singly.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP
Zzzz\CCG90.UAS.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (1)