UASt regulatory sequences are fused upstream of the EGFP version of Avic\GFP. A fragment containing 90 Zzzz\CCG repeats has been inserted between the transcriptional and translational start sites of Avic\GFP. The fragment containing the 90 Zzzz\CCG repeats is the same fragment as that contained within the P{UAS-CGG90-EGFP} construct, but it is inserted in reverse orientation in the P{UAS-CCG90-EGFP} construct.
Expression of Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PU results in a disorganised eye phenotype, with defects ranging from compressed ommatidia to disorganised bristles and fused ommatidia. Cross-sections of the eye show severe disruption and detachment of the retina and loss of photoreceptor cells.
Expression of Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Bx-MS1096 results in crumpled wings.
Expression of Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4C164 results in lethality in highly-expressing lines. Lower levels of expression result in flies that show a more rapid decline in their climbing ability with age than wild-type flies.
Scer\GAL4GMR.PU, Zzzz\CCG90.UAS.EGFP has visible phenotype, suppressible by Zzzz\CGG90.UAS.EGFP/Scer\GAL4GMR.PU
Zzzz\CCG90.UAS.EGFP/Scer\GAL4GMR.PU is a suppressor of visible phenotype of Scer\GAL4GMR.PU, Zzzz\CGG90.UAS.EGFP
AGO2414, Scer\GAL4GMR.PU, Zzzz\CCG90.UAS.EGFP, Zzzz\CGG90.UAS.EGFP has lethal | pupal stage phenotype
Scer\GAL4GMR.PU, Zzzz\CCG90.UAS.EGFP has eye photoreceptor cell phenotype, suppressible by Zzzz\CGG90.UAS.EGFP/Scer\GAL4GMR.PU
Scer\GAL4GMR.PU, Zzzz\CCG90.UAS.EGFP has eye phenotype, suppressible by Zzzz\CGG90.UAS.EGFP/Scer\GAL4GMR.PU
Zzzz\CCG90.UAS.EGFP/Scer\GAL4GMR.PU is a suppressor of eye phenotype of Scer\GAL4GMR.PU, Zzzz\CGG90.UAS.EGFP
Zzzz\CCG90.UAS.EGFP/Scer\GAL4GMR.PU is a suppressor of eye photoreceptor cell phenotype of Scer\GAL4GMR.PU, Zzzz\CGG90.UAS.EGFP
Co-expression of both Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP and Zzzz\CGG90.Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PU suppresses the disorganised external eye phenotype and photoreceptor cell degeneration which is seen when either construct is expressed singly under the control of Scer\GAL4GMR.PU. This mutual suppression by Zzzz\CCG90.Scer\UAS.T:Avic\GFP-EGFP and Zzzz\CGG90.Scer\UAS.T:Avic\GFP-EGFP does not occur if they are co-expressed under the control of Scer\GAL4GMR.PU in a AGO2414 homozygous background - in this case, the flies show pupal lethality and have a more severe eye phenotype than that seen when either construct is expressed singly.