FlyBase Allele Report: Dmel\Arfip[12]

General Information

Symbol

Dmel\Arfip¹²

Species

D. melanogaster

Name

FlyBase ID

FBal0281696

Feature type

allele

Associated gene

Dmel\Arfip

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Chang et al., 2013)

amorphic allele - molecular evidence

(Chang et al., 2013, Peru Y Colón de Portugal et al., 2012)

Mutagen

P-element activity

(Peru Y Colón de Portugal et al., 2012)

Progenitor genotype

P{RS3}Arfip^UM-8176-3

(Peru Y Colón de Portugal et al., 2012)

Cytology

Description

Imprecise excision of the progenitor insertion, deleting nucleotides 3R:7059346..7060959 (release 5 coordinates), including the first two exons and start codon of Arfip.

(Peru Y Colón de Portugal et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:11,233,624..11,235,237 [-]

Comment:

1613 bp deletion resulting from the imprecise excision of P{RS3}Arfip^UM-8176-3, which removes the first two exons and the start codon of @Arfip.

(Peru Y Colón de Portugal et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage (with Arfip⁷¹)

(Chang et al., 2015)

Golgi apparatus (with Arfip⁷¹)

(Chang et al., 2013)

NMJ bouton | second instar larval stage (with Arfip⁷¹)

(Chang et al., 2013)

NMJ bouton | third instar larval stage

(Chang et al., 2013)

NMJ bouton | third instar larval stage (with Arfip⁷¹)

(Chang et al., 2015, Chang et al., 2013)

Detailed Description

Statement

Reference

Arfip¹²/Arfip⁷¹ third instar larvae have significantly fewer boutons at the neuromuscular junction (muscle 4) compared to Arfip¹²/+ heterozygotes. Arfip¹²/Arfip⁷¹ larval NMJs are similar to wild type in terms ofRMP, mEJP, EJP and quantal content.

(Chang et al., 2015)

Newly eclosed homozygous escapers have no obvious neurological phenotypes and are able to fly and climb walls.

The average number of boutons at the neuromuscular junction is significantly reduced compared to controls in homozygous and Arfip⁷¹/Arfip¹² third instar larvae. The average number of boutons at the neuromuscular junction is also reduced in Arfip⁷¹/Arfip¹² second instar larvae.

Axonal transport is normal in Arfip⁷¹/Arfip¹² third instar larvae.

The Golgi appears smaller and more fragmented than normal in the ventral nerve cord of Arfip⁷¹/Arfip¹² third instar larvae.

(Chang et al., 2013)

Homozygotes are sensitive to ethanol-induced sedation compared to heterozygous siblings and wild-type controls.

Arfip¹²/Arfip⁷¹ flies are sensitive to ethanol-induced sedation compared to wild-type controls.

(Peru Y Colón de Portugal et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Arfip⁷¹/Arfip¹² has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by garz[+]/garz^Δ137

(Chang et al., 2015)

Enhancer of

Statement

Reference

Arfip¹² is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of DCTN1-p150^Δ.UAS, Scer\GAL4^Toll-6-D42

(Chang et al., 2013)

NOT Enhancer of

Statement

Reference

Arfip¹²/Arfip¹² is a non-enhancer of chemical sensitive phenotype of Arf6^NP5226/Arf6^EP2612

(Peru Y Colón de Portugal et al., 2012)

Arfip¹² is a non-enhancer of chemical resistant phenotype of Arf6^UAS.cCa, Scer\GAL4^elav-C155

(Peru Y Colón de Portugal et al., 2012)

Suppressor of

Statement

Reference

Arfip¹²/Arfip[+] is a suppressor of partially lethal - majority die phenotype of RhoGAP18B^NP1514

(Gonzalez et al., 2018)

Arfip¹²/Arfip[+] is a suppressor | partially of chemical resistant phenotype of RhoGAP18B¹

(Peru Y Colón de Portugal et al., 2012)

NOT Suppressor of

Statement

Reference

Arfip¹² is a non-suppressor of chemical resistant phenotype of Arf6^UAS.cCa, Scer\GAL4^elav-C155

(Peru Y Colón de Portugal et al., 2012)

Other

Statement

Reference

Arfip¹², Arl1^UAS.Tag:HA, Df(3L)Arl1-FRT, Scer\GAL4^RapGAP1-OK6 has abnormal neuroanatomy | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², Arl1[+]/Arl1¹ has abnormal neuroanatomy | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², Df(3L)Arl1-FRT/+ has abnormal neuroanatomy | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², garz[+]/garz^Δ137 has abnormal neuroanatomy | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², Gl[+]/DCTN1-p150^KG07739 has abnormal neuroanatomy | larval stage phenotype

(Chang et al., 2013)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Arfip⁷¹/Arfip¹² has NMJ bouton | third instar larval stage phenotype, non-enhanceable by garz[+]/garz^Δ137

(Chang et al., 2015)

Arfip⁷¹/Arfip¹² has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by garz[+]/garz^Δ137

(Chang et al., 2015)

Enhancer of

Statement

Reference

Arfip¹² is an enhancer of type Ib terminal bouton | third instar larval stage phenotype of DCTN1-p150^Δ.UAS, Scer\GAL4^Toll-6-D42

(Chang et al., 2013)

Other

Statement

Reference

Arfip¹², garz[+]/garz^Δ137 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², Arl1^UAS.Tag:HA, Df(3L)Arl1-FRT, Scer\GAL4^RapGAP1-OK6 has NMJ bouton | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², Arl1^UAS.Tag:HA, Df(3L)Arl1-FRT, Scer\GAL4^RapGAP1-OK6 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², Arl1[+]/Arl1¹ has NMJ bouton | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², Arl1[+]/Arl1¹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², Df(3L)Arl1-FRT/+ has NMJ bouton | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², Df(3L)Arl1-FRT/+ has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², garz[+]/garz^Δ137 has NMJ bouton | third instar larval stage phenotype

(Chang et al., 2015)

Arfip¹², Gl[+]/DCTN1-p150^KG07739 has NMJ bouton phenotype

(Chang et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Arfip¹²/Arl1¹ or Arfip¹²/Df(3L)Arl1-FRT third instar larvae have significantly fewer boutons at the neuromuscular junction compared to Arfip¹²/+ or Arl1¹/+ heterozygotes.

Expression of Arl1^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^RapGAP1-OK6 rescues the reduced bouton number seen at the neuromuscular junction of Arfip¹²/Df(3L)Arl1-FRT third instar larvae.

garz^Δ137/+;Arfip⁷¹/+ or garz^Δ137/+;Arfip¹²/+ third instar larvae have significantly fewer boutons at the neuromuscular junction compared to Arfip⁷¹/+ heterozygotes. garz^Δ137/+ does not enhance the reduction in bouton number seen in Arfip⁷¹/Arfip¹² larvae.

(Chang et al., 2015)

Arfip¹²/Gl^KG07739 double heterozygous larvae have a significantly reduced number of boutons at the neuromuscular junction.

Arfip¹² enhances the reduction in the number of type 1b boutons at the neuromuscular junction seen in third-instar larvae expressing Gl^Δ.Scer\UAS under the control of Scer\GAL4^D42.

(Chang et al., 2013)

Arfip¹²/+ partially suppresses the resistance to ethanol-induced sedation seen in RhoGAP18B¹ homozygotes.

RhoGAP18B¹/+ ; Arfip¹²/+ double heterozygotes show wild-type sensitivity to ethanol in a sedation assay.

The sensitivity to ethanol-induced sedation seen in Arf51F^NP5226/Arf51F^EP2612 flies is not enhanced if they are also homozygous for Arfip¹².

Arfip¹² does not alter the increased resistance to ethanol-induced sedation which is seen in flies expressing Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 specifically in the adult (using the heat sensitive Scer\GAL80^ts.αTub84B allele to limit expression).

(Peru Y Colón de Portugal et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Arfip⁷¹/Arfip¹² is rescued by Arfip^UAS.WT/Scer\GAL4^RapGAP1-OK6

(Chang et al., 2015)

Arfip⁷¹/Arfip¹² is rescued by Scer\GAL4^elav-C155/Arfip^UAS.WT

(Chang et al., 2013)

Arfip⁷¹/Arfip¹² is rescued by Arfip^UAS.WT/Scer\GAL4^RapGAP1-OK6

(Chang et al., 2013)

Arfip⁷¹/Arfip¹² is rescued by Arfip^UAS.Tag:HA/Scer\GAL4^hs.PU

(Peru Y Colón de Portugal et al., 2012)

Not rescued by

Arfip⁷¹/Arfip¹² is not rescued by Arfip^{GBD.UAS.Tag:HA}/Scer\GAL4^RapGAP1-OK6

(Chang et al., 2015)

Arfip⁷¹/Arfip¹² is not rescued by Scer\GAL4^elav-C155/Arfip^{GBD.UAS.Tag:HA}

(Chang et al., 2015)

Arfip⁷¹/Arfip¹² is not rescued by Scer\GAL4^elav-C155/Arfip^{BAR.UAS.Tag:HA}

(Chang et al., 2013)

Arfip⁷¹/Arfip¹² is not rescued by Scer\GAL4^RapGAP1-OK6/Arfip^{BAR.UAS.Tag:HA}

(Chang et al., 2013)

Comments

Expression of Arfip^Scer\UAS.WT driven by Scer\GAL4^RapGAP1-OK6 (motor-neuron specific) rescues the reduced bouton number seen at the neuromuscular junction of Arfip¹²/Arfip⁷¹ third instar larvae. Expression of Arfip^{GBD.Scer\UAS.T:Ivir\HA1} by either Scer\GAL4^elav-C155 (pan-neuronal) or Scer\GAL4^RapGAP1-OK6 does not rescue the reduced bouton number seen at the neuromuscular junction of Arfip¹²/Arfip⁷¹ third instar larvae.

(Chang et al., 2015)

Expression of Arfip^Scer\UAS.WT under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^Rapgap1-OK6 rescues the reduction in bouton number at the neuromuscular junction seen in Arfip⁷¹/Arfip¹² third instar larvae.

Expression of Arfip^{BAR.Scer\UAS.T:Ivir\HA1} under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^Rapgap1-OK6 fails to rescue the reduction in bouton number at the neuromuscular junction seen in Arfip⁷¹/Arfip¹² third instar larvae.

(Chang et al., 2013)

Expression of Arfip^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.PU at 29[o]C rescues the sensitivity of Arfip¹²/Arfip⁷¹ flies to ethanol-induced sedation.

(Peru Y Colón de Portugal et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

79623

w¹¹¹⁸; Arfip¹²/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Arfip¹²

(Peru Y Colón de Portugal et al., 2012)

Arfip^x12

(Gonzalez et al., 2018)

arfip¹²

(Chang et al., 2015, Chang et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close