FlyBase Allele Report: Dmel\Arf6[UAS.cCa]

General Information

Symbol

Dmel\Arf6^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Chen

FlyBase ID

FBal0176397

Feature type

allele

Associated gene

Dmel\Arf6

Associated Insertion(s)

Carried in Construct

P{UAS-Arf6.C}

Also Known As

UAS-Arf6

Key Links

Transgenic product class

wild_type

(Chen et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chen et al., 2003)

Mutagen

in vitro construct

(Chen et al., 2003)

Progenitor genotype

Arf6^UAS.cCa

(Chen et al., 2003)

Carried in construct

P{UAS-Arf6.C}

Cytology

Description

UASt sequences drive expression of Arf51F.

(Chen et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Arf6

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape, with Scer\GAL4^en-e16E

(Neisch et al., 2013)

chemical resistant, with Scer\GAL4^elav-C155

(Peru Y Colón de Portugal et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^en-e16E-mediated expression of Arf51F^Scer\UAS.cCa has very little effect on the wing disc epithelium, besides slightly altering cell shape.

(Neisch et al., 2013)

Expression of Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 specifically in the adult (using the heat sensitive Scer\GAL80^ts.αTub84B allele to limit expression) results in flies which are resistant to ethanol-induced sedation compared to wild-type controls.

(Peru Y Colón de Portugal et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Arf6^UAS.cCa, Scer\GAL4^elav-C155 has chemical resistant phenotype, non-enhanceable by Arfip¹²

(Peru Y Colón de Portugal et al., 2012)

NOT suppressed by

Statement

Reference

Arf6^UAS.cCa, Scer\GAL4^elav-C155 has chemical resistant phenotype, non-suppressible by Arfip¹²

(Peru Y Colón de Portugal et al., 2012)

Enhancer of

Statement

Reference

Arf6^UAS.cCa, Scer\GAL4^en-e16E is an enhancer of neoplasia phenotype of Scer\GAL4^en-e16E, conu^{UAS.Tag:Myr(Unk),Tag:FLAG}

(Neisch et al., 2013)

NOT Enhancer of

Statement

Reference

Arf6^UAS.cCa, Scer\GAL4^en-e16E is a non-enhancer of neoplasia phenotype of Scer\GAL4^en-e16E, conu^{UAS.Tag:Myr(Unk),Tag:FLAG}

(Neisch et al., 2013)

NOT Suppressor of

Statement

Reference

Arf6^UAS.cCa, Scer\GAL4^ppk.PU is a non-suppressor of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4^ppk.PU, Sec71^HMS00357

(Wang et al., 2017)

Arf6^UAS.cCa, Scer\GAL4^en-e16E is a non-suppressor of neoplasia phenotype of Scer\GAL4^en-e16E, conu^{UAS.Tag:Myr(Unk),Tag:FLAG}

(Neisch et al., 2013)

Other

Statement

Reference

Arf6^UAS.cCa, Scer\GAL4^en-e16E, conu^UAS.Tag:FLAG has neoplasia phenotype

(Neisch et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

Arf6^UAS.cCa, Scer\GAL4^en-e16E is an enhancer of wing disc phenotype of Scer\GAL4^en-e16E, conu^{UAS.Tag:Myr(Unk),Tag:FLAG}

(Neisch et al., 2013)

Arf6^UAS.cCa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Rac1^GMR.PN

(Neisch et al., 2013)

NOT Enhancer of

Statement

Reference

Arf6^UAS.cCa, Scer\GAL4^en-e16E is a non-enhancer of wing disc phenotype of Scer\GAL4^en-e16E, conu^{UAS.Tag:Myr(Unk),Tag:FLAG}

(Neisch et al., 2013)

NOT Suppressor of

Statement

Reference

Arf6^UAS.cCa, Scer\GAL4^ppk.PU is a non-suppressor of larval dorsal multidendritic neuron ddaC | pupal stage phenotype of Scer\GAL4^ppk.PU, Sec71^HMS00357

(Wang et al., 2017)

Arf6^UAS.cCa, Scer\GAL4^ppk.PU is a non-suppressor of dendritic tree | pupal stage phenotype of Scer\GAL4^ppk.PU, Sec71^HMS00357

(Wang et al., 2017)

Arf6^UAS.cCa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Rho1^UAS.cMa, Scer\GAL4^GMR.PF

(Neisch et al., 2013)

Arf6^UAS.cCa, Scer\GAL4^en-e16E is a non-suppressor of wing disc phenotype of Scer\GAL4^en-e16E, conu^{UAS.Tag:Myr(Unk),Tag:FLAG}

(Neisch et al., 2013)

Other

Statement

Reference

Arf6^UAS.cCa, Scer\GAL4^en-e16E, conu^UAS.Tag:FLAG has wing disc phenotype

(Neisch et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Arf51F^Scer\UAS.cCa does not suppress the pruning defects at 16h-18h after puparium formation observed in dorsal multidendritic ddaC neurons expressing Sec71^HMS00357 under the combined control of Scer\GAL4^ppk.PU and Dicer-2 transgene.

(Wang et al., 2017)

Co-expression of Arf51F^Scer\UAS.cCa strongly enhances the overproliferation phenotype seen in Scer\GAL4^en-e16E conu^{Scer\UAS.T:Uuuu\Myr5,T:Zzzz\FLAG} wing discs.

Co-expression of Arf51F^Scer\UAS.cCa with conu^{Scer\UAS.T:Zzzz\FLAG} results in an overproliferation and epithelial folding phenotype in wing discs.

Scer\GAL4^GMR.PF-mediated expression of Arf51F^Scer\UAS.cCa strongly enhances the rough eye of Rac1^GMR.PN flies.

(Neisch et al., 2013)

Arfip¹² does not alter the increased resistance to ethanol-induced sedation which is seen in flies expressing Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 specifically in the adult (using the heat sensitive Scer\GAL80^ts.αTub84B allele to limit expression).

(Peru Y Colón de Portugal et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Expression of Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^{Arf51F-NP5226} rescues the sensitivity of Arf51F^NP5226/Arf51F^EP2612 flies to ethanol-induced sedation. This rescue is suppressed in the presence of Scer\GAL80^elav.PY.

Expression of Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^elav.PLu rescues the sensitivity of Arf51F^KG02753/Arf51F^EP2612 flies to ethanol-induced sedation.

Expression of Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^{RhoGAP18B-NP1514} throughout development but not in the adult (using the heat sensitive Scer\GAL80^ts.αTub84B allele to limit expression) does not rescue the sensitivity of Arf51F^KG02753/Arf51F^EP2612 flies to ethanol-induced sedation.

Expression of Arf51F^Scer\UAS.cCa under the control of Scer\GAL4^{RhoGAP18B-NP1514} specifically in the adult (using the heat sensitive Scer\GAL80^ts.αTub84B allele to limit expression) does rescue the sensitivity of Arf51F^KG02753/Arf51F^EP2612 flies to ethanol-induced sedation.

(Peru Y Colón de Portugal et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Arf51F^Scer\UAS.cCa

Arf51F^UAS.cCa

Arf6^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Chen

(Chen et al., 2003)

Secondary FlyBase IDs

References (5)

Report Sections

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