FlyBase Allele Report: Dmel\Sec71[HMS00357]

General Information

Symbol

Dmel\Sec71^HMS00357

Species

D. melanogaster

Name

FlyBase ID

FBal0248122

Feature type

allele

Associated gene

Dmel\Sec71

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00357}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00357}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00357

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PU

(Wang et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Sec71^HMS00357 under the combined control of Scer\GAL4^ppk.PU and Dicer-2 transgene (for efficient RNAi), leads to dorsal multidendritic ddaC neurons exhibiting a significantly simplified dendritic tree at the white pupa stage and a fully penetrant delay in pruning at 16h-18h after puparium formation, as compared to controls; at the wandering third instar larval stage, these neurons exhibiting defects in the formation of secretory vesicles, as shown by the virtual absence of punctate structures for the exocyst marker Sec15, as compared to controls.

(Wang et al., 2017)

Expression via Scer\GAL4^fkh.PU results in larval salivary glands that have significantly smaller secretory granules, compared to controls.

(Torres et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PU, Sec71^HMS00357 has abnormal neuroanatomy | pupal stage phenotype, suppressible by Arf1^UAS.RR, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has abnormal neuroanatomy | pupal stage phenotype, suppressible | partially by Nrg^GL00656, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has abnormal neuroanatomy | pupal stage phenotype, suppressible | partially by Nrg^HMS01638, Scer\GAL4^ppk.PU

(Wang et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.PU, Sec71^HMS00357 has abnormal neuroanatomy | pupal stage phenotype, non-suppressible by Arf1^Q71L.UAS.cWa, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has abnormal neuroanatomy | pupal stage phenotype, non-suppressible by Arf6^UAS.cCa, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PU, Sec71^HMS00357 has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by Nrg^HMS01638, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has dendritic tree | pupal stage phenotype, suppressible | partially by Nrg^HMS01638, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible by Arf1^UAS.RR, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has dendritic tree | pupal stage phenotype, suppressible by Arf1^UAS.RR, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by Nrg^GL00656, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has dendritic tree | pupal stage phenotype, suppressible | partially by Nrg^GL00656, Scer\GAL4^ppk.PU

(Wang et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.PU, Sec71^HMS00357 has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, non-suppressible by Arf1^Q71L.UAS.cWa, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has dendritic tree | pupal stage phenotype, non-suppressible by Arf1^Q71L.UAS.cWa, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, non-suppressible by Arf6^UAS.cCa, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Scer\GAL4^ppk.PU, Sec71^HMS00357 has dendritic tree | pupal stage phenotype, non-suppressible by Arf6^UAS.cCa, Scer\GAL4^ppk.PU

(Wang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Both the simplified dendritic arborization at the white pupa stage and the pruning defects at 16h-18h after puparium formation observed in dorsal multidendritic ddaC neurons expressing Sec71^HMS00357 under the combined control of Scer\GAL4^ppk.PU and Dicer-2 transgene are suppressed by the co-expression of Arf79F^{Scer\UAS.RNAi-resistant}; the pruning defects at 16h after puparium formation are also partially suppressed by the co-expression of either Nrg^GL00656 or Nrg^HMS01638, but not Arf79F^{Q71L.Scer\UAS.cWa} or Arf51F^Scer\UAS.cCa.

(Wang et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sec71^HMS00357 is rescued by Scer\GAL4^ppk.PU/Sec71^UAS.RR

(Wang et al., 2017)

Sec71^HMS00357 is rescued by Sec71^ΔHDS2-4.UAS/Scer\GAL4^ppk.PU

(Wang et al., 2017)

Sec71^HMS00357 is rescued by Scer\GAL4^ppk.PU/Sec71^ΔHDS3-4.UAS

(Wang et al., 2017)

Partially rescued by

Sec71^HMS00357 is partially rescued by Sec71^ΔDCB.UAS/Scer\GAL4^ppk.PU

(Wang et al., 2017)

Comments

Both the dendritic arborization defects at the white pupa stage and the dendrite pruning defects at 16h after puparium formation observed in dorsal multidendritic ddaC neurons upon the expression of Sec71^HMS00357 under the combined control of Scer\GAL4^ppk.PU and Dicer-2 transgene are fully rescued by the co-expression of Sec71^{Scer\UAS.RNAi-resistant}, Sec71^{ΔHDS2-4.Scer\UAS} or Sec71^{ΔHDS3-4.Scer\UAS}; the co-expression of Sec71^{ΔDCB.Scer\UAS}, however, only partially rescues the dendritic arborization defects at the white pupa stage, but not the pruning defects at 16h after puparium formation.

(Wang et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

32366

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00357}attP2/TM3, Sb¹

NIG-Fly

HMS00357

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00357}attP2/TM3, Sb¹

Notes on Origin