FB2024_03 , released June 25, 2024
Allele: Dmel\Arf1UAS.RR
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General Information
Symbol
Dmel\Arf1UAS.RR
Species
D. melanogaster
Name
FlyBase ID
FBal0326764
Feature type
allele
Associated gene
Dmel\Arf1
Associated Insertion(s)
Carried in Construct
P{UAS-Arf1.RR}
Key Links
Transgenic product class
wild_type
(Wang et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Wang et al., 2017)
Mutagen
in vitro construct
(Wang et al., 2017)
Progenitor genotype
Carried in construct
P{UAS-Arf1.RR}
Cytology
Description

UAS regulatory sequences drive expression of the wild-type Arf79F protein. The construct has been engineered to be RNA-resistant by introducing a silent mutation on the third base of each codon.

(Wang et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Arf1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Arf79FScer\UAS.RNAi-resistant, either under the control of Scer\GAL4ppk.PU alone or under the combined control of Scer\GAL4GSG2295 and RU486 treatment from mid-third instar larval stage onwards, leads to dorsal multidendritic ddaC neurons exhibiting no obvious defects in the dendritic arborization at the white pupa stage or in dendrite pruning at 16h-18h after puparium formation, as compared to controls.

      (Wang et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of Arf79FScer\UAS.RNAi-resistant suppresses both the simplified dendritic arborization at the white pupa stage and the pruning defects at 16h-18h after puparium formation observed in dorsal multidendritic ddaC neurons expressing Sec71HMS00357 under the combined control of Scer\GAL4ppk.PU and Dicer-2 transgene.

      (Wang et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4ppk.PU/Arf1UAS.RR rescues Arf1GD12522

      (Wang et al., 2017)
      Comments

      The co-expression of Arf79FScer\UAS.RNAi-resistant fully rescues the dendritic arborization and dendrite pruning defects of dorsal multidendritic ddaC neurons resulting from the expression of Arf79FGD12522 under the control of Scer\GAL4ppk.PU in combination with Dicer-2 transgene.

      (Wang et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Arf1UAS.RNAi-resistant
      Arf1UAS.RR
      Arf79FScer\UAS.RNAi-resistant
      Arf79FUAS.RNAi-resistant
      Name Synonyms
      Secondary FlyBase IDs
        References (4)