FB2024_03 , released June 25, 2024
Allele: Dmel\Arf1Q71L.UAS.cWa
Open Close
General Information
Symbol
Dmel\Arf1Q71L.UAS.cWa
Species
D. melanogaster
Name
FlyBase ID
FBal0326763
Feature type
allele
Associated gene
Dmel\Arf1
Associated Insertion(s)
Carried in Construct
P{UAS-Arf1.Q71L.W}
Also Known As
UASt-Arf1Q71L
Key Links
Transgenic product class
missense_variant
(Wang et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Wang et al., 2017)
Mutagen
in vitro construct
(Wang et al., 2017)
Progenitor genotype
Carried in construct
P{UAS-Arf1.Q71L.W}
Cytology
Description

UAS regulatory sequences drive expression of a GTP-locked form of Arf79F (carries the amino acid replacement Q71L).

(Wang et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Arf1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Arf79FQ71L.Scer\UAS.cWa under the combined control of Scer\GAL4GSG2295 and RU486 treatment from mid-third instar larval stage onwards leads to dorsal multidendritic ddaC neurons exhibiting a fully penetrant and severe defect in dendrite pruning, as shown by the persistence of some dendritic branches at 16h-18h after puparium formation, but no obvious defects in the dendritic tree at the white pupa stage, as compared to controls.

      (Wang et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of Arf79FQ71L.Scer\UAS.cWa does not suppress the pruning defects at 16h-18h after puparium formation observed in dorsal multidendritic ddaC neurons expressing Sec71HMS00357 under the combined control of Scer\GAL4ppk.PU and Dicer-2 transgene.

      (Wang et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Arf1Q71L.UAS.cWa
      Arf79FQ71L.Scer\UAS.cWa
      Arf79FQ71L.UAS.cWa
      Name Synonyms
      Secondary FlyBase IDs
        References (3)