UAS regulates expression of wild type eya with the Tag:Myr(v-Src) myristoylation sequence inserted at the N-terminus.
Scer\GAL4dpp.blk1-mediated expression of eyaWT.Scer\UAS.T:Uuuu\Myr4 does not induce ectopic eyes.
Co-expression of eyaWT.Scer\UAS.T:SV40\nls2 and eyaWT.Scer\UAS.T:Uuuu\Myr4 via Scer\GAL4dpp.blk1 results in approximately two-fold more ectopic eye induction relative to expression of eyaWT.Scer\UAS.T:SV40\nls2 alone.
Scer\GAL4GMR.PU eyaWT.Scer\UAS.T:Uuuu\Myr4 eye/brain complexes show an average of 19.0 overshooting axon bundles per brain, compared to 8.4 in controls.
Scer\GAL4GMR.PU, eyaWT.UAS.Tag:Myr(v-Src) has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by AblGD1344, Scer\GAL4GMR.PU
Scer\GAL4GMR.PU, eyaWT.UAS.Tag:Myr(v-Src) has embryonic/larval brain | third instar larval stage phenotype, suppressible by AblGD1344, Scer\GAL4GMR.PU
Coexpression of AblGD1344 decreases the number of axon targeting defects in Scer\GAL4GMR.PU eyaWT.Scer\UAS.T:Uuuu\Myr4 eye/brain complexes from 19.0 to 8.7 axon bundles per brain.
eyaWT.UAS.Tag:NLS(SV40-largeT), Scer\GAL4dpp.blk1, eyaWT.UAS.Tag:Myr(v-Src) partially rescues eya2
Scer\GAL4dpp.blk1/eyaWT.UAS.Tag:Myr(v-Src) fails to rescue eya2
Scer\GAL4dpp.blk1-mediated expression of eyaWT.Scer\UAS.T:Uuuu\Myr4 does not rescue the eyeless phenotype of eya2 animals.
Co-expression of eyaWT.Scer\UAS.T:SV40\nls2 and eyaWT.Scer\UAS.T:Uuuu\Myr4 via Scer\GAL4dpp.blk1 results in a more marked rescue of the eyeless phenotype of eya2 animals relative to expression of eyaWT.Scer\UAS.T:SV40\nls2 alone.