FB2024_03 , released June 25, 2024
Allele: Dmel\eagDN.EKI.UAS
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General Information
Symbol
Dmel\eagDN.EKI.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0288214
Feature type
allele
Associated gene
Dmel\eag
Associated Insertion(s)
Carried in Construct
P{UAS-eag.DN.EKI}
Also Known As
UAS-eagΔ932, UAS-eag(DN), EKI
Key Links
Transgenic product class
dominant_negative_variant
(Duch et al., 2008)
inframe_deletion
(Broughton et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Duch et al., 2008)
inframe_deletion
(Broughton et al., 2004)
Mutagen
in vitro construct
(Duch et al., 2008, Broughton et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-eag.DN.EKI}
Cytology
Description

UAS regulatory sequences drive expression of a dominant-negative form of eag.

(Duch et al., 2008)

Deletion of all but the first 195 amino acids, followed by 14 novel residues DPGLVPRPQHRHRP. The resulting deleted protein is expressed from UASt regulatory sequences.

(Broughton et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
eag
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  brain cancer
      modeled by dpnUAS.cWa
      (Huang et al., 2015)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of eagDN.EKI.Scer\UAS under the control of Scer\GAL4futsch-C380 in the presence of Scer\GAL80Cha.PK (to suppress expression in all cholinergic neurons) slightly increases the excitability of the MN5 neuron. The firing response is still phasic, but more spikes occur in response to to a current injection of defined amplitude (although the amplitudes of the additional spikes are smaller).

      (Duch et al., 2008)

      When expression is driven by Scer\GAL4hs.PB with heat shock or Scer\GAL4D42 flies shows ether-induced leg shaking similar to that caused by eag1. When expression is driven by Scer\GAL4MJ286 or Scer\GAL4MJ146 the mean courstship latency is decreased, when driven by Scer\GAL4MJ63 the mean courstship latency is increased. Scer\GAL4MJ286 causes increase in mean duration of courtship. When expression is driven by Scer\GAL47B, Scer\GAL46J3, Scer\GAL453B, Scer\GAL440B, Scer\GAL428A, Scer\GAL4c309, Scer\GAL4c747, Scer\GAL4OK348, Scer\GAL429B, Scer\GAL4MJ63 or Scer\GAL4MJ146 wing extension and vibration duration during courtship are increased.

      (Broughton et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Suppressor of
      Other
      Phenotype Manifest In
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of eagDN.EKI.Scer\UAS markedly reduces dpnScer\UAS.cWa-induced tumor growth.

      (Huang et al., 2015)

      Co-expression of eagDN.EKI.Scer\UAS and ShDN.EKI.Scer\UAS.T:Avic\GFP-GL in the RP2 motor neurons (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU) results in a significant increase in the number of dendritic branches and increased dendritic complexity near the neuronal soma.

      (Timmerman et al., 2013)

      Co-expression of ShDN.EKI.Scer\UAS and eagDN.EKI.Scer\UAS under the control of Scer\GAL4D42 in the presence of Scer\GAL80Cha.PK (to suppress expression in cholinergic neurons) results in a significant increase in total dendritic length and in the number of dendritic branches in MN5 motorneurons.

      The reduction in total dendritic length and in the number of dendritic branches which is seen in MN5 motorneurons in animals expressing JraJbz.Scer\UAS under the control of Scer\GAL4D42 in the presence of Scer\GAL80Cha.PK (to suppress expression in cholinergic neurons) is not altered if the flies are also simultaneously co-expressing both ShDN.EKI.Scer\UAS and eagDN.EKI.Scer\UAS.

      (Vonhoff et al., 2013)

      Co-expression of both ShDN.EKI.Scer\UAS and eagDN.EKI.Scer\UAS under the control of Scer\GAL4futsch-C380 in the presence of Scer\GAL80Cha.PK (to suppress expression in all cholinergic neurons) results in the MN5 neuron showing a tonic firing response to a current injection into the soma. Resting membrane potential and input resistance of the MN5 neuron are unaffected.

      The dendritic structure of the MN5 neuron is altered compared to wild type in animals expressing ShDN.EKI.Scer\UAS and eagDN.EKI.Scer\UAS under the control of Scer\GAL4futsch-C380. Total dendritic length, the number of branch points and the the mean length of individual dendritic branches is increased. Maximum branch order is unaffected. The mean distance of all dendritic segments to the origin of the tree and the total dendritic surface are increased.

      Flies expressing both ShDN.EKI.Scer\UAS and eagDN.EKI.Scer\UAS under the control of Scer\GAL4futsch-C380 in the presence of Scer\GAL80Cha.PK show increased flight motor performance in a restrained flight assay. The initial and total flight time are significantly increased compared to wild type.

      (Duch et al., 2008)

      Motor neurons co-expressing eagDN.EKI.Scer\UAS and ShDN.EKI.Scer\UAS.T:Avic\GFP-GL under the control of Scer\GAL4futsch-C380 (in the presence of Scer\GAL80Cha.PK) have similar action potential thresholds as control neurons in response to a depolarising current, but have a significantly higher action potential frequency in response to a positive current. The postspike hyperpolarisation is smaller in the mutant larvae, often resulting in spike doublets.

      Larval RP2 motor neurons co-expressing eagDN.EKI.Scer\UAS and ShDN.EKI.Scer\UAS.T:Avic\GFP-GL under the control of Scer\GAL4unspecified show a significant increase in dendrite volume compared to controls.

      Cultured motor neurons co-expressing eagDN.EKI.Scer\UAS and ShDN.EKI.Scer\UAS.T:Avic\GFP-GL under the control of Scer\GAL4futsch-C380 (in the presence of Scer\GAL80Cha.PK to suppress expression in cholinergic neurons) show a significant increase in both neurite length and branch number compared to controls.

      (Hartwig et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference

      Simultaneous co-expression of both eagDN.EKI.Scer\UAS and ShDN.EKI.Scer\UAS.T:Avic\GFP-GL enhances the progressive reduction in electroretinogram (ERG) amplitude which is seen in flies expressing Hsap\LRRK2G2019S.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ple.PG, such that the triple mutant flies show a significant reduction in ERG amplitude at 10 days of age.

      (Hindle et al., 2013)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      eagDN.EKI.Scer\UAS
      eagDN.EKI.UAS
      eagDN
      (Huang et al., 2015)
      eagΔ932.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0282704
      • FBal0155360
      References (10)