FB2024_03 , released June 25, 2024
Allele: Dmel\gusUAS.L.Tag:HA
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General Information
Symbol
Dmel\gusUAS.L.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0288297
Feature type
allele
Associated gene
Dmel\gus
Associated Insertion(s)
Carried in Construct
P{UAS-gus.L.HA}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Kugler et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-gus.L.HA}
Cytology
Description

UAS regulatory sequences drive expression of a 349-amino-acid long isoform of gus tagged with a Tag:HA epitope.

(Kugler et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
gus
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of gusScer\UAS.L.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PU fails to rescue the collapsed-egg phenotype of homozygous maternal gusf07073 mutants. In fact, embryos of homozygous gusf07073 mothers containing both the gusScer\UAS.L.T:Ivir\HA1 transgene and Scer\GAL4Act5C.PU are never recovered.

      Maternal overexpression of gusScer\UAS.L.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 causes a significant reduction in the number of primordial germ cells in embryos compared with wild-type.

      Some eggs produced from Scer\GAL4nos.UTR.T:Hsim\VP16>gusScer\UAS.L.T:Ivir\HA1-overexpressing females are unfertilized, and some embryos develop aberrantly.

      (Kugler et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4Act5C.PU/gusUAS.L.Tag:HA fails to rescue gusf07073

      (Kugler et al., 2010)
      Comments

      Expression of gusScer\UAS.L.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PU fails to rescue the collapsed-egg phenotype of homozygous maternal gusf07073 mutants.

      (Kugler et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      gusScer\UAS.L.T:Ivir\HA1
      gusUAS.L.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)