FlyBase Allele Report: Dmel\α-Cat[UASp.ΔVH2N.Tag:HA]

General Information

Symbol

Dmel\α-Cat^{UASp.ΔVH2N.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0294100

Feature type

allele

Associated gene

Dmel\α-Cat

Associated Insertion(s)

Carried in Construct

P{UASp-α-Cat.ΔVH2N.HA}

Key Links

Transgenic product class

inframe_deletion

(Desai et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Desai et al., 2013)

Mutagen

in vitro construct

(Desai et al., 2013)

Progenitor genotype

Carried in construct

P{UASp-α-Cat.ΔVH2N.HA}

Cytology

Description

UASp regulatory sequences drive expression of a mutant form of α-Cat lacking amino acids 398-509. The construct is tagged at the C-terminal end with Tag:HA.

(Desai et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

α-Cat

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/first instar larval cuticle | embryonic stage, with α-Cat^HMS00317, Scer\GAL4^{VP16.mat.αTub67C}

(Sheppard et al., 2023)

ventral denticle belt | embryonic stage, with α-Cat^HMS00317, Scer\GAL4^{VP16.mat.αTub67C}

(Sheppard et al., 2023)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

α-Cat^HMS00317, α-Cat^{UASp.ΔVH2N.Tag:HA}, Scer\GAL4^{VP16.mat.αTub67C} has embryonic/first instar larval cuticle | embryonic stage phenotype, enhanceable by cno^TRiP.cUa, Scer\GAL4^{VP16.mat.αTub67C}

(Sheppard et al., 2023)

Suppressed by

Statement

Reference

α-Cat^HMS00317, α-Cat^{UASp.ΔVH2N.Tag:HA}, Scer\GAL4^{VP16.mat.αTub67C} has ventral denticle belt | embryonic stage phenotype, suppressible | partially by cno^UASp.GFP, Scer\GAL4^{VP16.mat.αTub67C}

(Sheppard et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

α-Cat^{UASp.ΔVH2N.Tag:HA}/Scer\GAL4^{VP16.mat.αTub67C} partially rescues α-Cat^HMS00317

(Sheppard et al., 2023)

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, α-Cat^{UASp.ΔVH2N.Tag:HA} partially rescues α-Cat^Ubi.PS

(Desai et al., 2013)

Comments

Expression of α-Cat^{Scer\UAS.P\T.ΔVH2N.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are partially rescued.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.ΔVH2N.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. No adults are seen but the animals are able to develop beyond the embryonic stages, with some animals reaching the pupal stage.

(Desai et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

65597

w¹¹¹⁸; P{UASp-α-Cat.ΔVH2N.HA}attP2

65585

w¹¹¹⁸; P{tubP-GAL80}LL10, P{Ubi-α-Cat.S}4A P{neoFRT}40A; P{Act-GAL4.U}3, P{UASp-α-Cat.ΔVH2N.HA}attP2 α-Cat¹/TM6B, Tb¹

Notes on Origin

Discoverer