FlyBase Allele Report: Hsap\YARS1[WT.UAS]

General Information

Symbol

Hsap\YARS1^WT.UAS

Species

H. sapiens

Name

FlyBase ID

FBal0297459

Feature type

allele

Associated gene

Hsap\YARS1

Associated Insertion(s)

Carried in Construct

P{UAS-hYARS.S}

PBac{UAS-YARS.WT}

Also Known As

YARS_WT

Key Links

Transgenic product class

cDNA

(Storkebaum et al., 2009)

wild_type

(Storkebaum et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Storkebaum et al., 2009)

wild_type

(Storkebaum et al., 2009)

Mutagen

in vitro construct

(Niehues et al., 2015, Storkebaum et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-hYARS.S}

PBac{UAS-YARS.WT}

Cytology

Description

Scer\UAS regulates expression of full-length wild type Hsap\YARS cDNA.

(Niehues et al., 2015)

UASt regulatory sequences drive expression of full-length Hsap\YARS cDNA clone IMAGp998G1110070Q.

(Storkebaum et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\YARS1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Scer\GAL4^Act5C.PI

(Bervoets et al., 2019)

viable, with Scer\GAL4^Act5C.PU

(Storkebaum et al., 2009)

viable, with Scer\GAL4^Tub.PU

(Storkebaum et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Hsap\YARS1^WT.UAS under the control of Scer\GAL4^GMR.long does not have a major effect on retinal morphology, as compared to controls.

The expression under the control of Scer\GAL4^nSyb.PU also does not affect adult climbing ability, or third instar larval NMJ morphology (size and bouton number).

The expression under the control of Scer\GAL4^GMR91H05 also does not affect giant fiber morphology, or the giant fiber ability to follow repeated stimuli.

(Bervoets et al., 2019)

Expression of Hsap\YARS^Scer\UAS.cSa under the control of either Scer\GAL4^Act5C.PU or Scer\GAL4^tub.PU results in normal adult offspring.

Pan-neuronal expression of Hsap\YARS^Scer\UAS.cSa under the control of Scer\GAL4^elav.PU has no effect on motor performance in negative gravitaxis assays.

No jumping and flying defects are seen when Hsap\YARS^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Act5C.PU.

No obvious degeneration is observed when Hsap\YARS^Scer\UAS.cSa is expressed under the control of Scer\GAL4^nSyb.PS.

(Storkebaum et al., 2009)

Flies expressing one or two copies of Hsap\YARS^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PU show normal behaviour in a negative geotaxis climbing assay.

Flies expressing two copies of Hsap\YARS^Scer\UAS.cSa under the control of Scer\GAL4^nSyb.PS show normal behaviour in a negative geotaxis climbing assay.

Aged flies expressing Hsap\YARS^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PU are able to jump and fly.

Flies expressing two copies of Hsap\YARS^Scer\UAS.cSa under the control of Scer\GAL4^OK307 show normal electrophysiological responses in the giant fiber system.

(Storkebaum et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^GMR.long, Hsap\YARS1^WT.UAS is a non-enhancer of visible | adult stage phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.long

(Bervoets et al., 2019)

Hsap\YARS1^WT.UAS, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PU, corolla^d01774

(Ermanoska et al., 2014)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.long, Hsap\YARS1^WT.UAS is a non-suppressor of visible | adult stage phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.long

(Bervoets et al., 2019)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^GMR.long, Hsap\YARS1^WT.UAS is a non-enhancer of eye phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.long

(Bervoets et al., 2019)

Hsap\YARS1^WT.UAS, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PU, corolla^d01774

(Ermanoska et al., 2014)

Suppressor of

Statement

Reference

Hsap\YARS1^WT.UAS, Scer\GAL4^sca.PU is a suppressor of scutellar bristle phenotype of Scer\GAL4^sca.PU, TyrRS^NIG.4561R

(Storkebaum et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.long, Hsap\YARS1^WT.UAS is a non-suppressor of eye phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^GMR.long

(Bervoets et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\YARS^Scer\UAS.cSa does not enhance the mild rough eye phenotype seen when CG8316^d01774 is expressed under the control of Scer\GAL4^GMR.PU.

(Ermanoska et al., 2014)

The bristle defects caused by expression of Aats-tyr^NIG.4561R under the control of Scer\GAL4^sca.PU are completely rescued by co-expression of Hsap\YARS^Scer\UAS.cSa.

(Storkebaum et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Hsap\YARS1^WT.UAS

Hsap\YARS^Scer\UAS.cSa

Hsap\YARS^WT.Scer\UAS

Hsap\YARS^WT.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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