FB2024_03 , released June 25, 2024
Allele: Dmel\goe5-11
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General Information
Symbol
Dmel\goe5-11
Species
D. melanogaster
Name
FlyBase ID
FBal0301370
Feature type
allele
Associated gene
Dmel\goe
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Matsuoka et al., 2014)
Mutagen
P-element activity
(Matsuoka et al., 2014)
Progenitor genotype
P{EPgy2}goeEY01697
(Matsuoka et al., 2014)
Cytology
Description

Imprecise excision of P{EPgy2}goeEY01697 resulting in the deletion of a 92 bp segment encompassing a 63 bp upstream genomic sequence and part of exon 1 including the transcriptional start site of goe.

(Matsuoka et al., 2014)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
X:16,751,882..16,751,973 [-]
Comment:

Inferred boundaries of a 92 bp deletion resulting from the imprecise excision of P{EPgy2}goeEY01697, which removes 63 bp upstream of goe transcription start site and part of the first exon.

(Matsuoka et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ovaries in third instar goe5-11/goe331 larvae display an approximately 1.5-fold increase in the number of differentiating germ cells compared with wild-type. Compared with wild-type, he number of undifferentiated primordial germ cells (PGCs) remains unchanged in goe5-11/goe331 ovaries, resulting in a net increase in the total number of germ cells. Neither the mitotic index of PGCs nor the apoptotic profile show any detectable changes in goe5-11/goe331 ovaries compared to wild-type. The mutant ovaries show an increase in the number of germline cysts undergoing dedifferentiation.

      (Matsuoka et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      goe5-11/goe331 has female germline cell | third instar larval stage phenotype, enhanceable by aos[+]/aosΔ7

      (Matsuoka et al., 2014)
      Other
      Statement
      Reference

      aos[+]/aosΔ7, goe5-11/goe331 has female germline stem cell phenotype

      (Matsuoka et al., 2014)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      aosΔ7 dominantly enhances the increased differentiating germ cell phenotype in goe5-11/goe331 mutant larval ovaries.

      While neither goe5-11/goe331 nor aosΔ7/+ ovaries exhibit a remarkable reduction in the number of germline stem cells (GSCs) at white pupal stage, establishment is severely impaired in goe5-11/goe331; aosΔ7/+ ovaries. This reduction in GSC number is likely the result of an insufficient primordial germ cell pool, because both ovariole formation and niche development occur normally in goe5-11/goe331; aosΔ7/+ ovaries.

      (Matsuoka et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      goe5-11
      (Matsuoka et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)