Over-expression of goe^Scer\UAS.cMa using the germline-specific driver Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in decreased number of germ cells initiating gametogenesis in third instar larvae. In contrast to controls, these ovaries contain fewer cystoblasts and developing germline cysts. Because the total number of germ cells is indistinguishable from controls, the decrease in the number of differentiating germ cells is likely the result of from a reduction in the fraction of germ cells that are allocated to gametogenesis. In support of this, a significant increase in the number of undifferentiated primordial germ cells (PGCs) is observed as a result of Scer\GAL4^{nos.UTR.T:Hsim\VP16}>goe^Scer\UAS.cMa over-expression. By contrast, the number of mitotic and apoptotic cells is essentially the same as those in control ovaries, suggesting that over-expression of goe^Scer\UAS.cMa does not affect the proliferation and survival of germ cells. These results suggest that goe^Scer\UAS.cMa can suppress PGC differentiation in L3 ovaries.

Overexpression of goe^Scer\UAS.cMa in the entire wing disc using Scer\GAL4^nub.PU causes a reduction in adult wing size compared with wild-type.