FB2024_03 , released June 25, 2024
Allele: Dmel\Gr5aLEXA
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General Information
Symbol
Dmel\Gr5aLEXA
Species
D. melanogaster
Name
FlyBase ID
FBal0304286
Feature type
allele
Associated gene
Dmel\Gr5a
Associated Insertion(s)
TI{lexA::VP16}Gr5aLEXA
Carried in Construct
Also Known As
Gr5a-lexA
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Yavuz et al., 2014)
Mutagen
gene targeting by homologous recombination
(Fujii et al., 2015)
Progenitor genotype
Associated Insertion(s)
TI{lexA::VP16}Gr5aLEXA
Cytology
Description

The Gr5a coding sequence has been replaced with sequence encoding a lexA::VP16 driver.

(Fujii et al., 2015)

The Gr5a coding sequence has been replaced with sequence encoding a lexA::VP16 driver. A single loxP site is also present downstream of the inserted driver sequence.

(Yavuz et al., 2014)
Allele components
Component
Use(s)
Inserted element
TI{lexA::VP16}
Encoded product / tool
lexA::VP16
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous Gr5aLEXA mutant flies exhibit a reduced proboscis extension response to maltose and trehalose upon stimulation of either the labial or tarsal taste sensilla, and several other sugars upon stimulation of the tarsal sensilla.

      (Fujii et al., 2015)

      Gr61a1 Gr5aLEXA Df(3L)ΔGr64 mutants expressing P{t15.3.R1} and P{UAS-Rescue.R2} (i.e. lacking all 8 sweet gustatory receptor genes) do not exhibit any defects in fructose preference in two-choice preference assays.

      (Mishra et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, suppressible | partially by Scer\GAL4Gr61a.4.9/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, suppressible | partially by Scer\GAL4Gr61a.4.9/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, suppressible | partially by Scer\GAL4Gr61a.4.9/Gr61aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, suppressible | partially by Scer\GAL4Gr61a.4.9/Gr61aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, suppressible | partially by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa/Gr64eUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, suppressible | partially by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa/Gr64eUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, suppressible | partially by Scer\GAL4Gr61a.4.9/Gr64aUAS.cUa/Gr64eUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, suppressible | partially by Scer\GAL4Gr61a.4.9/Gr64aUAS.cUa/Gr64eUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, suppressible | partially by Gr5aUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, suppressible | partially by Gr5aUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)
      NOT suppressed by
      Statement
      Reference

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Gr64dUAS.cUa/Scer\GAL4Gr61a.4.9/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, non-suppressible by Gr64dUAS.cUa/Scer\GAL4Gr61a.4.9/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Gr64cUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, non-suppressible by Gr64cUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Gr64dUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, non-suppressible by Gr64dUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64eUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64eUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Gr64fUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has abnormal taste perception | adult stage phenotype, non-suppressible by Gr64fUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)
      Other
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5b phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5v phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5b phenotype, non-suppressible by Gr64cUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5v phenotype, non-suppressible by Gr64cUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5b phenotype, non-suppressible by Gr64dUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5v phenotype, non-suppressible by Gr64dUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5b phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64eUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5v phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64eUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5b phenotype, non-suppressible by Gr64fUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5v phenotype, non-suppressible by Gr64fUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5b phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5v phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5b phenotype, non-suppressible by Gr64dUAS.cUa/Scer\GAL4Gr61a.4.9/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5v phenotype, non-suppressible by Gr64dUAS.cUa/Scer\GAL4Gr61a.4.9/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5b phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5b phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr61aUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5v phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64bUAS.cUa/Gr64eUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5v phenotype, non-suppressible by Scer\GAL4Gr61a.4.9/Gr64aUAS.cUa/Gr64eUAS.cUa

      (Yavuz et al., 2014)

      Df(3L)ΔGr64, Gr5aLEXA, Gr61a1 has sugar-sensing neuron of prothoracic leg tarsal taste bristle 5b phenotype, non-suppressible by Gr5aUAS.cUa/Scer\GAL4Gr61a.4.9

      (Yavuz et al., 2014)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Adult females homozygous for Gr5aLEXA, Gr61a1 and Df(3L)ΔGr64, or with Gr5aLEXA/+, Gr61a1/+ and Gr64fLEXA/Df(3L)ΔGr64, have a significant increase in preference for softer 100 mM sucrose-containing, but not plain, agarose for egg laying, compared to controls; these mutants also have a significant reduction in preference for 100 mM sucrose-containing (rather than plain) agarose for egg laying, compared to controls.

      (Wu et al., 2019)

      Adults with homozygous Gr5aLEXA, Gr61a1 and Df(3L)ΔGr64 have proboscis extension rates that are significantly reduced in response to sucrose and significantly increased in response to some fatty acids, compared to controls.

      (Ahn et al., 2017)

      Gr5aLEXA, Gr61a1, Df(3L)ΔGr64 triple homozygotes that also bear P{t15.3.R1} (P{t15.3.R1} rescues the lethality of Df(3L)ΔGr64 homozygosity) have impaired responsiveness to sweet chemical: sweet taste 5b sensilla GRNs virtually lack the expected responsiveness to the chemicals Sucrose, Fructose, Maltose, Trehalose, Glucose, Melezitose, Arabinose and Glycerol, as compared to controls; sweet taste 5v sensilla GRNs show the expected responsive only to Sucrose and Fructose, but lack responses to Maltose, Trehalose and Glucose.

      Expressing Gr64aUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores the response of 5v sensilla GRNs only to Maltose but does not affect the responses of 5b sensilla GRNs.

      Expressing Gr61aUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores a moderate response of 5v sensilla GRNs only to Glucose but does not affect the responses of 5b sensilla GRNs.

      Expressing Gr5aUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores a small response of 5v sensilla GRNs only to Trehalose but does not affect the responses of 5b sensilla GRNs.

      Co-expressing Gr64aUAS.cUa and Gr64eUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores the response of 5b sensilla GRNs only to Maltose and Sucrose, but does not affect the responses by 5v sensilla GRNs.

      Co-expressing Gr64bUAS.cUa and Gr64eUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores the response of 5b sensilla GRNs only to Glycerol, but does not affect the responses by 5v sensilla GRNs.

      (Yavuz et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Gr5aLEXA
      (Kim et al., 2018, Fujii et al., 2015, Yavuz et al., 2014)
      Gr5aLexA
      (Chen et al., 2022, Wu et al., 2019, Ahn et al., 2017, Mishra et al., 2013)
      ΔGr5a
      (Devineni et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (9)