FlyBase Allele Report: Dmel\p53[-ns]

General Information

Symbol

Dmel\p53^-ns

Species

D. melanogaster

Name

FlyBase ID

FBal0304383

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

TI{TI}p53^-ns

Carried in Construct

Also Known As

dp53^ns, p53^ns, dmp53^-ns

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

ends-in gene targeting

(Sogame et al., 2003)

FLPase

(Sogame et al., 2003)

I-SceI

(Sogame et al., 2003)

Progenitor genotype

Associated Insertion(s)

TI{TI}p53^-ns

Cytology

Description

Duplication at the p53 locus. The 5' copy consists of wild-type exons 1 to 6, a partial insertion of pTV2 vector sequence, another copy of exon 6 and part of exon 7. The 3' copy lacks exon 1 and part of exon 2. A w^+mW.hs marker and a single FRT site are located between the two copies.

(Sogame et al., 2003)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with p53^TM3, Hsap\TP53^R175H

(D'Brot et al., 2017)

CEA with p53^TM3, Hsap\TP53^G245S

(D'Brot et al., 2017)

CEA with p53^TM3, Hsap\TP53^R248Q

(D'Brot et al., 2017)

CEA with p53^TM3, Hsap\TP53^R273C

(D'Brot et al., 2017)

CEA with p53^TM3, Hsap\TP53^R273H

(D'Brot et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mitochondrion | somatic clone

(de la Cova et al., 2014)

oocyte | oogenesis stage S2 (with p53²)

(Lu et al., 2010)

oocyte | oogenesis stage S2 (with p53^11-1B-1)

(Lu et al., 2010)

primordial germ cell (with p53^5A-1-4)

(Yamada et al., 2008)

wing disc | embryonic stage | conditional (with p53^TM3)

(D'Brot et al., 2017)

wing disc | larval stage | conditional

(D'Brot et al., 2017)

wing disc | larval stage | conditional (with p53^TM3)

(D'Brot et al., 2017)

wing disc | somatic clone

(de la Cova et al., 2014)

Detailed Description

Statement

Reference

γ-irradiated p53^-ns/p53^TM3 embryos and larval wing discs (the Tm3, Sb balancer has an inversion break point at the p53 gene), as well as γ-irradiated p53^-ns homozygous wing discs, fail to show the expected increase in apoptosis.

(D'Brot et al., 2017)

Homozygous Dp(3;3)p53^-ns mutant flies (comprised of the p53^-ns.5' and p53^-ns.3' alleles) mutant flies exhibit a reduction in survival following acute starvation during early adulthood (1-2 days). Reduced survival rates are seen in both males and females and also in 5-7 day old flies subjected to starvation. Similar effect on survival rates are seen in young adult flies or larvae exposed to complete nutrient deprivation, as well as larvae exposed to diluted food. Triacylglycerides (TAGs) and glycogen are used at higher rates in p53^-ns.3' mutant flies exposed to nutrient deprivation compared to controls.

Homozygous p53^-ns flies show a reduction in survival following acute starvation during early adulthood (1-2 days). Reduced survival rates are seen in both males and females and also in 5-7 day old flies subjected to starvation. Similar effects on survival rates are seen in young adult flies or larvae exposed to complete nutrient deprivation, as well as larvae exposed to diluted food. Triacylglycerides (TAGs) and glycogen are used at higher rates in mutant flies exposed to nutrient deprivation compared to controls.

Homozygous Dp(3;3)p53^-ns mutant flies (comprised of the p53^-ns.5' and p53^-ns.3' alleles) mutant flies exhibit a reduction in survival following acute starvation during early adulthood (1-2 days). Reduced survival rates are seen in both males and females and also in 5-7 day old flies subjected to starvation. Similar effects on survival rate are seen in young adult flies or larvae exposed to complete nutrient deprivation, as well as larvae exposed to diluted food. Triacylglycerides (TAGs) and glycogen are used at higher rates in p53^-ns.3' mutant flies exposed to nutrient deprivation compared to controls.

(Barrio et al., 2014)

p53^-ns mutant wing disc clones grow to a much larger size than control cell clones.

p53^-ns mutant wing disc cells or fat body cells exhibit disrupted mitochondrial morphology, with a variety of shapes including globules and donut shapes as well as long filaments, as seen in wild-type.

p53^-ns.5' mutant wing disc clones grow to a much larger size than control cell clones.

p53^-ns.5' mutant wing disc cells or fat body cells exhibit disrupted mitochondrial morphology, with a variety of shapes including globules and donut shapes as well as long filaments, as seen in wild-type.

(de la Cova et al., 2014)

p53^-ns heterozygous mutants show apparently normal thorax structure.

p53^-ns.5' heterozygous mutants show apparently normal thorax structure.

(Tue et al., 2011)

Meiotic recombination frequency is reduced in p53^11-1B-1/p53^-ns and p53²/p53^-ns mutants compared with wild type.

(Lu et al., 2010)

p53^-ns mutants are viable, but sickly with a shortened life span.

p53^-ns.3',p53^-ns.5' mutants are viable, but sickly with a shortened life span.

(Bauer et al., 2005)

p53^-ns mutants do not exhibit the normal increase in apoptosis following irradiation. However cell cycle arrest occurs as normal. Low doses of ionising radiation cause an increase in genomic instability and an increase in lethality when compared to controls.

p53^-ns.3',p53^-ns.5' mutants do not exhibit the normal increase in apoptosis following irradiation. However cell cycle arrest occurs as normal. Low doses of ionising radiation cause an increase in genomic instability and an increase in lethality when compared to controls.

(Sogame et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

p53^TM3/p53^-ns has decreased cell death | larval stage | conditional phenotype, suppressible by Hsap\TP53^WT.1

(D'Brot et al., 2017)

p53^TM3/p53^-ns has decreased cell death | embryonic stage | conditional phenotype, suppressible by Hsap\TP53^WT.1

(D'Brot et al., 2017)

p53^TM3/p53^-ns has decreased cell death | larval stage | conditional phenotype, suppressible by Hsap\TP53^WT.2

(D'Brot et al., 2017)

p53^TM3/p53^-ns has decreased cell death | embryonic stage | conditional phenotype, suppressible by Hsap\TP53^WT.2

(D'Brot et al., 2017)

p53^-ns has decreased cell death | larval stage | conditional phenotype, suppressible by Hsap\TP53^WT.2

(D'Brot et al., 2017)

NOT suppressed by

Statement

Reference

p53^-ns has decreased cell death | embryonic stage | conditional phenotype, non-suppressible by Hsap\TP53^R175H

(D'Brot et al., 2017)

p53^-ns has decreased cell death | embryonic stage | conditional phenotype, non-suppressible by Hsap\TP53^G245S

(D'Brot et al., 2017)

p53^-ns has decreased cell death | embryonic stage | conditional phenotype, non-suppressible by Hsap\TP53^R248Q

(D'Brot et al., 2017)

p53^-ns has decreased cell death | embryonic stage | conditional phenotype, non-suppressible by Hsap\TP53^R273C

(D'Brot et al., 2017)

p53^-ns has decreased cell death | embryonic stage | conditional phenotype, non-suppressible by Hsap\TP53^R273H

(D'Brot et al., 2017)

Enhancer of

Statement

Reference

p53[+]/p53^-ns is an enhancer of visible | adult stage | dominant phenotype of N^55e11

(Simón et al., 2014)

p53[+]/p53^-ns is an enhancer of visible | adult stage | dominant phenotype of wg¹

(Simón et al., 2014)

NOT Enhancer of

Statement

Reference

p53^-ns is a non-enhancer of abnormal cell number | oogenesis phenotype of Scer\GAL4^bab1-Gal4, piwi^KK105350

(Lin et al., 2020)

Suppressor of

Statement

Reference

p53[+]/p53^-ns is a suppressor of abnormal starvation stress response phenotype of Dcr-1^Q1147X

(Barrio et al., 2014)

p53[+]/p53^-ns is a suppressor of increased cell death phenotype of Dref^UAS.cSa

(Trong-Tue et al., 2010)

p53^-ns/p53^-ns is a suppressor of increased cell death phenotype of Dref^UAS.cSa

(Trong-Tue et al., 2010)

p53^-ns is a suppressor of decreased occurrence of cell division | larval stage phenotype of BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4, hid^UAS.cYa

(Wells et al., 2006)

NOT Suppressor of

Statement

Reference

p53^-ns is a non-suppressor of abnormal cell number | oogenesis phenotype of Scer\GAL4^bab1-Gal4, piwi^KK105350

(Lin et al., 2020)

p53^-ns/p53^-ns is a non-suppressor of visible phenotype of AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH

(Joza et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

p53^TM3/p53^-ns has wing disc | larval stage | conditional phenotype, suppressible by Hsap\TP53^WT.1

(D'Brot et al., 2017)

p53^TM3/p53^-ns has wing disc | embryonic stage | conditional phenotype, suppressible by Hsap\TP53^WT.1

(D'Brot et al., 2017)

p53^TM3/p53^-ns has wing disc | larval stage | conditional phenotype, suppressible by Hsap\TP53^WT.2

(D'Brot et al., 2017)

p53^TM3/p53^-ns has wing disc | embryonic stage | conditional phenotype, suppressible by Hsap\TP53^WT.2

(D'Brot et al., 2017)

p53^-ns has wing disc | embryonic stage | conditional phenotype, suppressible by Hsap\TP53^WT.2

(D'Brot et al., 2017)

NOT suppressed by

Statement

Reference

p53^-ns has wing disc | embryonic stage | conditional phenotype, non-suppressible by Hsap\TP53^R175H

(D'Brot et al., 2017)

p53^-ns has wing disc | embryonic stage | conditional phenotype, non-suppressible by Hsap\TP53^G245S

(D'Brot et al., 2017)

p53^-ns has wing disc | embryonic stage | conditional phenotype, non-suppressible by Hsap\TP53^R248Q

(D'Brot et al., 2017)

p53^-ns has wing disc | embryonic stage | conditional phenotype, non-suppressible by Hsap\TP53^R273C

(D'Brot et al., 2017)

p53^-ns has wing disc | embryonic stage | conditional phenotype, non-suppressible by Hsap\TP53^R273H

(D'Brot et al., 2017)

Enhancer of

Statement

Reference

p53[+]/p53^-ns is an enhancer of wing phenotype of N^55e11

(Simón et al., 2014)

p53[+]/p53^-ns is an enhancer of wing phenotype of wg¹

(Simón et al., 2014)

p53[+]/p53^-ns is an enhancer of adult thorax phenotype of Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^pnr-MD237

(Tue et al., 2011)

NOT Enhancer of

Statement

Reference

p53^-ns is a non-enhancer of female germline stem cell phenotype of Scer\GAL4^bab1-Gal4, piwi^KK105350

(Lin et al., 2020)

p53^-ns is a non-enhancer of germarium phenotype of Scer\GAL4^bab1-Gal4, piwi^KK105350

(Lin et al., 2020)

Suppressor of

Statement

Reference

p53[+]/p53^-ns is a suppressor of eye phenotype of Dref^UAS.cSa

(Trong-Tue et al., 2010)

p53^-ns/p53^-ns is a suppressor of eye phenotype of Dref^UAS.cSa

(Trong-Tue et al., 2010)

NOT Suppressor of

Statement

Reference

p53^-ns is a non-suppressor of female germline stem cell phenotype of Scer\GAL4^bab1-Gal4, piwi^KK105350

(Lin et al., 2020)

p53^-ns is a non-suppressor of germarium phenotype of Scer\GAL4^bab1-Gal4, piwi^KK105350

(Lin et al., 2020)

p53^-ns/p53^-ns is a non-suppressor of eye phenotype of AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH

(Joza et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of p53^-ns suppresses the increase in survival rate in response to starvation seen in adult Dcr-1^Q1147X/+ mutant flies.

One copy of Dp(3;3)p53^-ns (comprised of the p53^-ns.5' and p53^-ns.3' alleles) suppresses the increase in survival rate in response to starvation seen in adult Dcr-1^Q1147X/+ mutant flies.

(Barrio et al., 2014)

A p53^-ns heterozygous background strongly enhances the thorax disclosed phenotype induced by Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} expression (under the control of Scer\GAL4^pnr-MD237).

(Tue et al., 2011)

A half reduction in the levels of p53 through crossing Scer\GAL4^GMR.PS/+; Dref^Scer\UAS.cSa/+ flies with p53^-ns.3' p53^-ns.5' flies suppresses the Dref^Scer\UAS.cSa-induced rough eye phenotype. This suppression is more evident in a p53^-ns.3' p53^-ns.5' homozygous background.

The rough eye phenotype caused by expression of Dref^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PS is suppressed by p53^-ns/+ and by p53^-ns/p53^-ns (with stronger suppression in the homozygous background).

(Trong-Tue et al., 2010)

The mutant eye phenotype caused by expression of AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH is not ameliorated if they are also homozygous for Dp(3;3)p53^-ns (which carries p53^-ns.5' and p53^-ns.3').

The mutant eye phenotype caused by expression of AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH is not ameliorated if they are also homozygous for p53^-ns.

(Joza et al., 2008)

Xenogenetic Interactions

Statement

Reference

The increase in the number of TUNEL-positive cells and the non-autonomous increase in BrdU incorporation seen in the wing discs in larvae expressing Rcom\RA^cs2.Scer\UAS under the control of Scer\GAL4^en-e16E at 30[o]C is largely suppressed by p53^-ns.

(Mesquita et al., 2010)

The cell cycle arrest and undergrowth phenotype of 96 hour wing discs with posterior 'undead' Scer\GAL4^hh-Gal4>hid^Scer\UAS.cYa, BacA\p35^Scer\UAS.cHa cells is completely alleviated in both p53^5A-1-4 and p53^-ns backgrounds.

The cell cycle arrest and undergrowth phenotype of 96 hour wing discs with posterior 'undead' Scer\GAL4^hh-Gal4>W^Scer\UAS.cYa, BacA\p35^Scer\UAS.cHa cells is completely alleviated in both p53^5A-1-4 and p53^-ns.3', p53^-ns.5' backgrounds.

(Wells et al., 2006)

Complementation and Rescue Data

Rescued by

p53^-ns is rescued by p53^{+tCH322-115D03}

(D'Brot et al., 2017)

p53^-ns is rescued by p53^GUS.PB

(Barrio et al., 2014)

Comments

Expression of p53^GUS.PB under the control of Scer\GAL4^yolk rescues the reduced survival seen in homozygous p53^-ns flies in response to starvation.

Expression of p53^GUS.PB under the control of Scer\GAL4^yolk rescues the reduced survival seen in homozygous Dp(3;3)p53^-ns mutant flies (comprised of the p53^-ns.5' and p53^-ns.3' alleles) in response to starvation.

(Barrio et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

23283

w¹¹¹⁸/Dp(1;Y)y⁺; TI{TI}p53^-ns

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

Dmp53^-ns

(Palomera-Sanchez et al., 2010, Sogame et al., 2003)

Dmp53^NS

(Akdemir et al., 2007, Bauer et al., 2005)

Dmp53^null

(Rebollar et al., 2006)

Dp(3;3)p53^-ns

Dp53^-ns

(Simón et al., 2014)

dmp53^-ns

(Chen et al., 2013)

dp53^NS

(D'Brot et al., 2017)

dp53^ns

(Barrio et al., 2014, Mesquita et al., 2010, Wells et al., 2006)

p53^-ns.3'

p53^-ns.5'

p53^-ns

(Lin et al., 2020, Miller et al., 2020, Trong-Tue et al., 2010, Yamada et al., 2008)

p53^NS

(Wylie et al., 2022)

p53^ns

(de la Cova et al., 2014, Wylie et al., 2014, Joza et al., 2008)

p53^null

(Rebollar et al., 2006)

Name Synonyms

Secondary FlyBase IDs

FBal0146964
FBal0146963
FBab0037620

References (21)

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