FlyBase Allele Report: Dmel\Nf-YA[RNAi.231-399.UAS.WIZ]

General Information

Symbol

Dmel\Nf-YA^{RNAi.231-399.UAS.WIZ}

Species

D. melanogaster

Name

FlyBase ID

FBal0221541

Feature type

allele

Associated gene

Dmel\Nf-YA

Associated Insertion(s)

Carried in Construct

P{UAS-Nf-YA.RNAi.231-399}

Key Links

Transgenic product class

RNAi_reagent

(Yoshioka et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Yoshioka et al., 2007)

Mutagen

in vitro construct

(Yoshioka et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Nf-YA.RNAi.231-399}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of Nf-YA sequences corresponding to amino acid residues 231-399.

(Yoshioka et al., 2008)

UAS regulatory sequences drive expression of an inverted repeat of a 510bp fragment of the C-terminal region of Nf-YA.

(Yoshioka et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nf-YA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^69B

(Yoshioka et al., 2007)

lethal, with Scer\GAL4^GMR.PS

(Yoshioka et al., 2012)

lethal | pharate adult stage, with Scer\GAL4^pnr-MD237

(Yoshioka et al., 2008)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

(Yoshioka et al., 2008)

lethal - all die before end of larval stage, with Scer\GAL4^Act5C.PU

(Yoshioka et al., 2007)

lethal - all die before end of larval stage, with Scer\GAL4^en-e16E

(Yoshioka et al., 2007)

lethal - all die before end of P-stage, with Scer\GAL4^GMR.PS

(Yoshioka et al., 2007)

some die during larval stage, with Scer\GAL4^Act5C.PU

(Yoshioka et al., 2007)

some die during larval stage, with Scer\GAL4^en-e16E

(Yoshioka et al., 2007)

some die during pharate adult stage, with Scer\GAL4^GMR.PS

(Yoshioka et al., 2007)

visible, with Scer\GAL4^ey.PH

(Yoshioka et al., 2007)

visible, with Scer\GAL4^GMR.PS

(Yoshioka et al., 2012)

Phenotype Manifest In

adult thorax, with Scer\GAL4^pnr-MD237

(Tue et al., 2011)

eye, with Scer\GAL4^ey.PH

(Yoshioka et al., 2007)

eye, with Scer\GAL4^GMR.PS

(Yoshioka et al., 2012)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Yoshioka et al., 2008)

Detailed Description

Statement

Reference

Expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^GMR.PS can result in lethality, or in a rough eye phenotype, depending on the insertion line used.

(Yoshioka et al., 2012)

Expression of Nf-YA^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^pnr-MD237 results in a thorax disclosed phenotype.

(Tue et al., 2011)

Animals expressing Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^pnr-MD237 die as pupae or pharate adults and have a cleft in the dorsal notum.

Co-expression of Nf-YA^{Scer\UAS.T:Ivir\HA1} suppresses the cleft notum phenotype caused by expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^pnr-MD237.

(Yoshioka et al., 2008)

Expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^ey.PH results in a small eye phenotype in 53% of cases.

Co-expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} in animals expressing Nf-YA^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ey.PH results in more than 90% viability and the flies have a normal head phenotype.

(Yoshioka et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^GMR.PS has visible phenotype, suppressible | partially by Raf^hs.PU

(Yoshioka et al., 2012)

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^GMR.PS has visible phenotype, suppressible | partially by sev^hs.PU

(Yoshioka et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^pnr-MD237 has adult thorax phenotype, enhanceable by p53[+]/p53^-ns

(Tue et al., 2011)

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^pnr-MD237 has mesothoracic tergum phenotype, enhanceable by bsk¹/bsk[+]

(Yoshioka et al., 2008)

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^pnr-MD237 has mesothoracic tergum phenotype, enhanceable by bsk²/bsk[+]

(Yoshioka et al., 2008)

NOT Enhanced by

Statement

Reference

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^pnr-MD237 has mesothoracic tergum phenotype, non-enhanceable by hep[+]/hep¹

(Yoshioka et al., 2008)

Suppressed by

Statement

Reference

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^GMR.PS has eye phenotype, suppressible | partially by Raf^hs.PU

(Yoshioka et al., 2012)

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^GMR.PS has eye phenotype, suppressible | partially by sev^hs.PU

(Yoshioka et al., 2012)

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^pnr-MD237 has mesothoracic tergum phenotype, suppressible by bsk^UAS.cAYa, Scer\GAL4^pnr-MD237

(Yoshioka et al., 2008)

NOT suppressed by

Statement

Reference

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^pnr-MD237 has mesothoracic tergum phenotype, non-suppressible by hep[+]/hep¹

(Yoshioka et al., 2008)

Nf-YA^{RNAi.231-399.UAS.WIZ}, Scer\GAL4^pnr-MD237 has mesothoracic tergum phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^pnr-MD237

(Yoshioka et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of either phl^hs.PU or sev^hs.PU suppresses the rough eye phenotype caused by expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^GMR.PS.

(Yoshioka et al., 2012)

A p53^-ns heterozygous background strongly enhances the thorax disclosed phenotype induced by Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} expression (under the control of Scer\GAL4^pnr-MD237).

(Tue et al., 2011)

The cleft notum phenotype caused by expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^pnr-MD237 is enhanced by bsk¹/+ or bsk²/+, is only marginally enhanced by hep^r75/+ and is not affected by hep¹/+.

The cleft notum phenotype caused by expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^pnr-MD237 is suppressed by co-expression of bsk^{Scer\UAS.cAYa}.

(Yoshioka et al., 2008)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa has no effect on the cleft notum phenotype caused by expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^pnr-MD237.

(Yoshioka et al., 2008)

Complementation and Rescue Data

Rescued by

Nf-YA^{RNAi.231-399.UAS.WIZ} is rescued by Scer\GAL4^GMR.PS/Nf-YA^UAS.Tag:HA

(Yoshioka et al., 2012)

Comments

Co-expression of Nf-YA^{Scer\UAS.T:Ivir\HA1} rescues the rough eye phenotype caused by expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^GMR.PS.

(Yoshioka et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nf-YA^{RNAi.231-399.UAS.WIZ}

Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ}

Nf-YA^{dsRNA.231-399.UAS.WIZ}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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