FlyBase Allele Report: Dmel\Nf-YA[UAS.Tag:HA]

General Information

Symbol

Dmel\Nf-YA^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0221540

Feature type

allele

Associated gene

Dmel\Nf-YA

Associated Insertion(s)

Carried in Construct

P{UAS-Nf-YA.HA}

Also Known As

UAS-HA-dNF-YA

Key Links

Transgenic product class

wild_type

(Yoshioka et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Yoshioka et al., 2007)

Mutagen

in vitro construct

(Yoshioka et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Nf-YA.HA}

Cytology

Description

UASt regulatory sequences drive expression of Nf-YA which is tagged with Tag:HA.

(Yoshioka et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Nf-YA

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antenna | ectopic, with Scer\GAL4^ey.PH

(Yoshioka et al., 2007)

eye, with Scer\GAL4^ey.PH

(Yoshioka et al., 2007)

head | pharate adult stage, with Scer\GAL4^ey.PH

(Yoshioka et al., 2007)

ommatidium, with Scer\GAL4^ey.PH

(Yoshioka et al., 2007)

Detailed Description

Statement

Reference

Animals expressing Nf-YA^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^pnr-MD237 have a normal notum phenotype.

Co-expression of Nf-YA^{Scer\UAS.T:Ivir\HA1} suppresses the cleft notum phenotype caused by expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^pnr-MD237.

(Yoshioka et al., 2008)

Pharate adults expressing Nf-YA^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ey.PH have a headless or small head phenotype. Adult escapers expressing Nf-YA^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ey.PH sometimes have duplicated antennas and small eyes, or have an increased number of ommatidia.

Co-expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} in animals expressing Nf-YA^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ey.PH results in more than 90% viability and the flies have a normal head phenotype.

(Yoshioka et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nf-YA^UAS.Tag:HA, Scer\GAL4^ey.PH has lethal phenotype, enhanceable by ey²

(Yoshioka et al., 2007)

NOT Enhanced by

Statement

Reference

Nf-YA^UAS.Tag:HA, Scer\GAL4^ey.PH has lethal phenotype, non-enhanceable by N^264-39

(Yoshioka et al., 2007)

Suppressed by

Statement

Reference

Nf-YA^UAS.Tag:HA, Scer\GAL4^ey.PH has lethal phenotype, suppressible | partially by Dll⁹

(Yoshioka et al., 2007)

NOT suppressed by

Statement

Reference

Nf-YA^UAS.Tag:HA, Scer\GAL4^ey.PH has lethal phenotype, non-suppressible by N^264-39

(Yoshioka et al., 2007)

Suppressor of

Statement

Reference

Nf-YA^UAS.Tag:HA, Scer\GAL4^Bx-MS1096 is a suppressor | partially of visible phenotype of Scer\GAL4^Bx-MS1096, ey^UAS.cHa

(Yoshioka et al., 2007)

Phenotype Manifest In

Suppressor of

Statement

Reference

Nf-YA^UAS.Tag:HA, Scer\GAL4^Bx-MS1096 is a suppressor | partially of wing phenotype of Scer\GAL4^Bx-MS1096, ey^UAS.cHa

(Yoshioka et al., 2007)

Nf-YA^UAS.Tag:HA, Scer\GAL4^Bx-MS1096 is a suppressor | partially of eye | ectopic phenotype of Scer\GAL4^Bx-MS1096, ey^UAS.cHa

(Yoshioka et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Dll⁹ flies expressing Nf-YA^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ey.PH show 86% viability.

Co-expression of Nf-YA^{Scer\UAS.T:Ivir\HA1} partially suppresses the induction of ectopic eye structures in the wing which is caused by expression of ey^Scer\UAS.cHa under the control of Scer\GAL4^Bx-MS1096.

(Yoshioka et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^GMR.PS/Nf-YA^UAS.Tag:HA rescues Nf-YA^{RNAi.231-399.UAS.WIZ}

(Yoshioka et al., 2012)

Comments

Co-expression of Nf-YA^{Scer\UAS.T:Ivir\HA1} rescues the rough eye phenotype caused by expression of Nf-YA^{dsRNA.231-399.Scer\UAS.WIZ} under the control of Scer\GAL4^GMR.PS.

(Yoshioka et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nf-YA^{Scer\UAS.T:Ivir\HA1}

Nf-YA^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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