FB2024_03 , released June 25, 2024
Allele: Dmel\SLC22AUAS.cGa
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General Information
Symbol
Dmel\SLC22AUAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Gai
FlyBase ID
FBal0319343
Feature type
allele
Associated gene
Dmel\SLC22A
Associated Insertion(s)
Carried in Construct
P{UAS-SLC22A.G}
Key Links
Transgenic product class
wild_type
(Gai et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Gai et al., 2016)
Mutagen
in vitro construct
(Gai et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-SLC22A.G}
Cytology
Description

UAS regulatory sequences drive expression of the SLC22A open reading frame.

(Gai et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
SLC22A
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ectopic expression of SLC22AScer\UAS.cGa in all mushroom body neurons using either the Scer\GAL4238Y or the Scer\GAL4GMR13F02 driver results in significant 3hr memory loss in an olfactory classical conditioning assay in adult flies.

      Odor (Benzaldehyde and Octanol) and shock (electrical) avoidance was normal (tested with Scer\GAL4238Y and Scer\GAL4GMR13F02).

      A conditional ectopic expression of SLC22AScer\UAS.cGa after eclosion in all mushroom body neurons using the Scer\GAL4238Y driver (with GAL80ts to limit the stage of expression) results in loss of 3hr memory in an olfactory classical conditioning assay whereas expression during development (before eclosion) has no effect on memory test performance in adults.

      Supplementing food with dopamine or histidine impairs 3hr memory of both control adult flies and of the already memory-defective flies expressing SLC22AScer\UAS.cGa under the control of the Scer\GAL4238Y driver in an olfactory classical conditioning assay.

      Supplementing food with choline impairs 3hr memory of control adult flies but improves that of the already memory-defective flies expressing SLC22AScer\UAS.cGa under the control of the Scer\GAL4238Y driver in an olfactory classical conditioning assay.

      (Gai et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4238Y/SLC22AUAS.cGa rescues SLC22AKK112539

      (Gai et al., 2016)

      Scer\GAL4GMR13F02/SLC22AUAS.cGa rescues SLC22AKK112539

      (Gai et al., 2016)
      Comments

      Co-expression of SLC22AScer\UAS.cGa with SLC22AKK112539 RNAi using either Scer\GAL4238Y or Scer\GAL4GMR13F02 driver fully rescues the 3hr enhanced memory phenotype in adult flies in an olfactory classical conditioning assay produced by the SLC22 knock-down.

      (Gai et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      SLC22AScer\UAS.cGa
      SLC22AUAS.cGa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Gai
      Secondary FlyBase IDs
        References (1)