UASt regulatory sequences drive expression of a CTPsyn C isoform cDNA that has been mutated to carry the amino acid replacement C399G. This is equivalent to the C404G mutation in the yeast Ura7p ortholog which results in a protein that cannot bind its glutamine substrate. The CTPsyn protein is tagged at the N-terminal end with Tag:FLAG.
Scer\GAL4hs.PU/CtpsC399G.UAS.Tag:FLAG is a non-suppressor of abnormal endomitotic cell cycle | somatic clone | adult stage phenotype of CblF165
Scer\GAL4hs.PU/CtpsC399G.UAS.Tag:FLAG is a suppressor of cytoophidium | female | somatic clone | adult stage phenotype of CblF165
Scer\GAL4hs.PU/CtpsC399G.UAS.Tag:FLAG is a suppressor of follicle cell | somatic clone | adult stage phenotype of CblF165
Scer\GAL4hs.PU/CtpsC399G.UAS.Tag:FLAG is a non-suppressor of follicle cell | somatic clone | adult stage phenotype of CblF165
The loss of cytoophidia observed in somatic clones of CblF165 homozygous mutant follicle cells is suppressed by expression of CTPsynC399G.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4hs.PU in the clones, although the CTPsynC399G.Scer\UAS.T:Zzzz\FLAG protein cannot be incorporated in the cytoophidia filament structure together with endogenous CTPsyn protein in wild-type cells.
The endocycle defects (decrease in DNA replication measured by BrdU incorporation) seen in somatic clones of CblF165 homozygous mutant follicle cells is not suppressed by expression of CTPsynC399G.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4hs.PU in the clones.