FB2024_03 , released June 25, 2024
Allele: Dmel\CtpsC399G.UAS.Tag:FLAG
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General Information
Symbol
Dmel\CtpsC399G.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0319932
Feature type
allele
Associated gene
Dmel\Ctps
Associated Insertion(s)
Carried in Construct
P{UAS-Flag-Ctps.C399G}
Key Links
Transgenic product class
missense_variant
(Wang et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Wang et al., 2015)
Mutagen
in vitro construct
(Wang et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-Flag-Ctps.C399G}
Cytology
Description

UASt regulatory sequences drive expression of a CTPsyn C isoform cDNA that has been mutated to carry the amino acid replacement C399G. This is equivalent to the C404G mutation in the yeast Ura7p ortholog which results in a protein that cannot bind its glutamine substrate. The CTPsyn protein is tagged at the N-terminal end with Tag:FLAG.

(Wang et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ctps
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4hs.PU/CtpsC399G.UAS.Tag:FLAG is a non-suppressor of follicle cell | somatic clone | adult stage phenotype of CblF165

      (Wang et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The loss of cytoophidia observed in somatic clones of CblF165 homozygous mutant follicle cells is suppressed by expression of CTPsynC399G.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4hs.PU in the clones, although the CTPsynC399G.Scer\UAS.T:Zzzz\FLAG protein cannot be incorporated in the cytoophidia filament structure together with endogenous CTPsyn protein in wild-type cells.

      The endocycle defects (decrease in DNA replication measured by BrdU incorporation) seen in somatic clones of CblF165 homozygous mutant follicle cells is not suppressed by expression of CTPsynC399G.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4hs.PU in the clones.

      (Wang et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      CTPsynC399G.Scer\UAS.T:Zzzz\FLAG
      CTPsynC399G.UAS.Tag:FLAG
      CtpsC399G.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)