FlyBase Allele Report: Dmel\tap[Gal4]

General Information

Symbol

Dmel\tap^Gal4

Species

D. melanogaster

Name

FlyBase ID

FBal0321568

Feature type

allele

Associated gene

Dmel\tap

Associated Insertion(s)

TI{GAL4}tap^Gal4

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Yuan et al., 2016)

Mutagen

FLPase

(Yuan et al., 2016)

ends-in gene targeting

(Yuan et al., 2016)

I-CreI

(Yuan et al., 2016)

I-SceI

(Yuan et al., 2016)

Progenitor genotype

Associated Insertion(s)

TI{GAL4}tap^Gal4

Cytology

Description

The tap open reading frame has been replaced by sequence encoding a GAL4 driver, such that GAL4 is expressed under the control of endogenous tap regulatory sequences.

(Yuan et al., 2016)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | dominant

(Yuan et al., 2016)

abnormal neuroanatomy | adult stage | somatic clone

(Yuan et al., 2016)

abnormal neuroanatomy | P-stage | dominant

(Yuan et al., 2016)

lethal - all die before end of larval stage

(Yuan et al., 2016)

majority die during embryonic stage

(Yuan et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

tap^Gal4/tap^Gal4 embryos show no obvious morphological defects, with the number and fate of neurons and glia not significantly altered in the CNS or PNS, compared to controls.

tap^Gal4/+ heterozygotic adults show a significant increase in the frequency of mild beta lobe axon midline crossing defects (seen from early pupal stages and in the adult), missing alpha lobes (in some of these cases, the beta lobe appears to branch into two bundles) and loss of dorsal projection of alpha/beta neurons in the mushroom bodies compared to wild type. In around one third of tap^Gal4/tap^Gal4 mushroom body clones, beta axons project beyond the mushroom body beta lobe domains (in severe cases, axons cross the midline and project to the contralateral lobe).

(Yuan et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

tap^Gal4 has abnormal neuroanatomy | adult stage | dominant phenotype, enhanceable by dsh¹/dsh[+]

(Yuan et al., 2016)

tap^Gal4 has abnormal neuroanatomy | adult stage | dominant phenotype, enhanceable by dsh[+]/dsh⁶

(Yuan et al., 2016)

NOT Enhanced by

Statement

Reference

tap^Gal4 has abnormal neuroanatomy | adult stage | dominant phenotype, non-enhanceable by Dscam1²¹/Dscam1[+]

(Yuan et al., 2016)

tap^Gal4 has abnormal neuroanatomy | adult stage | dominant phenotype, non-enhanceable by Abl⁴/Abl[+]

(Yuan et al., 2016)

tap^Gal4 has abnormal neuroanatomy | adult stage | dominant phenotype, non-enhanceable by Appl^d/Appl[+]

(Yuan et al., 2016)

tap^Gal4 has abnormal neuroanatomy | adult stage | dominant phenotype, non-enhanceable by stan[+]/stan^E59

(Yuan et al., 2016)

tap^Gal4 has abnormal neuroanatomy | adult stage | dominant phenotype, non-enhanceable by Vang^stbm-6/Vang[+]

(Yuan et al., 2016)

tap^Gal4 has abnormal neuroanatomy | adult stage | dominant phenotype, non-enhanceable by Wnt5⁴⁰⁰/Wnt5[+]

(Yuan et al., 2016)

tap^Gal4 has abnormal neuroanatomy | adult stage | dominant phenotype, non-enhanceable by drl[+]/drl^Red2

(Yuan et al., 2016)

tap^Gal4 has abnormal neuroanatomy | adult stage | dominant phenotype, non-enhanceable by sli²/sli[+]

(Yuan et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

tap^Gal4 has adult mushroom body alpha-lobe | adult stage phenotype, enhanceable by dsh[+]/dsh⁶

(Yuan et al., 2016)

tap^Gal4 has adult mushroom body beta-lobe | adult stage phenotype, enhanceable by dsh¹/dsh[+]

(Yuan et al., 2016)

tap^Gal4 has adult mushroom body beta-lobe | adult stage phenotype, enhanceable by dsh[+]/dsh⁶

(Yuan et al., 2016)

NOT Enhanced by

Statement

Reference

tap^Gal4 has adult mushroom body beta-lobe | adult stage phenotype, non-enhanceable by Dscam1²¹/Dscam1[+]

(Yuan et al., 2016)

tap^Gal4 has adult mushroom body alpha-lobe | adult stage phenotype, non-enhanceable by Abl⁴/Abl[+]

(Yuan et al., 2016)

tap^Gal4 has adult mushroom body alpha-lobe | adult stage phenotype, non-enhanceable by Appl^d/Appl[+]

(Yuan et al., 2016)

tap^Gal4 has adult mushroom body alpha-lobe | adult stage phenotype, non-enhanceable by stan[+]/stan^E59

(Yuan et al., 2016)

tap^Gal4 has adult mushroom body alpha-lobe | adult stage phenotype, non-enhanceable by Vang^stbm-6/Vang[+]

(Yuan et al., 2016)

tap^Gal4 has adult mushroom body alpha-lobe | adult stage phenotype, non-enhanceable by Wnt5⁴⁰⁰/Wnt5[+]

(Yuan et al., 2016)

tap^Gal4 has adult mushroom body beta-lobe | adult stage phenotype, non-enhanceable by drl[+]/drl^Red2

(Yuan et al., 2016)

tap^Gal4 has adult mushroom body beta-lobe | adult stage phenotype, non-enhanceable by sli²/sli[+]

(Yuan et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Presence of Dscam1²¹/+, drl^Red2/+, Abl⁴/+, Appl^d/+, stan^E59/+, Vang^stbm-6/+, Wnt5⁴⁰⁰/+ or sli²/+ does not significantly enhance frequency of mushroom body beta lobe axon overgrowth defects in tap^Gal4/+ brains. Presence of dsh¹/+ or dsh⁶/+ significantly enhances frequency of mushroom body beta lobe axon overgrowth defects (midline crossing) in tap^Gal4/+ brains; dsh⁶/+ also significantly enhances frequency of missing alpha lobes.

(Yuan et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

tap^Gal4 is rescued by tap^UAS.GFP/Scer\GAL4^tap-Gal4

(Yuan et al., 2016)

Comments

Expression of tap^{Scer\UAS.T:Avic\GFP} (driven by Scer\GAL4^tap-Gal4) rescues lethality and mushroom body defects in tap^Gal4/tap^Gal4 flies (or beta axon overgrowth in mutant clones).

(Yuan et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

tap^Gal4

(Yuan et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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