10x UAS regulates expression of the artificial transcriptional activator dCas9::VPR, which is composed of a nuclease-dead form of Spyo\Cas9 fused to a highly active transcription activation sequence. The driver is tagged at the N-terminus with 3x Tag:FLAG and nuclear localization signals are present either side of the mutated Spyo\Cas9 sequence. This driver is designed to be used to overexpress a target gene of interest from its endogenous genomic locus, by combining it with sgRNAs that target sequences upstream of the transcription start site of the gene of interest.
asRNA:CR43467sgRNA.UAS.RA1/Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG, Scer\GAL4GMR.PU is a suppressor of visible | adult stage phenotype of FIG4KK102943, Scer\GAL4GMR.PU
asRNA:CR43467sgRNA.UAS.RB1/Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG, Scer\GAL4GMR.PU is a suppressor of visible | adult stage phenotype of FIG4KK102943, Scer\GAL4GMR.PU
asRNA:CR43467sgRNA.UAS.RA2/Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG, Scer\GAL4GMR.PU is a suppressor of visible | adult stage phenotype of FIG4KK102943, Scer\GAL4GMR.PU
fruTOE.GS02668/Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG, Scer\GAL4Or47b.7.467 is a suppressor of abnormal neurophysiology | adult stage | conditional phenotype of MetKK104562, Scer\GAL4Or47b.7.467
fruTOE.GS02668, Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG, Scer\GAL4Or47b.7.467 is a suppressor of abnormal neurophysiology | adult stage | conditional phenotype of Scer\GAL4Or47b.7.467, nejRNAi.UAS.cSa
asRNA:CR43467sgRNA.UAS.RB1/Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG, Scer\GAL4GMR.PU is a suppressor of eye phenotype of FIG4KK102943, Scer\GAL4GMR.PU
asRNA:CR43467sgRNA.UAS.RA2/Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG, Scer\GAL4GMR.PU is a suppressor of eye phenotype of FIG4KK102943, Scer\GAL4GMR.PU
asRNA:CR43467sgRNA.UAS.RA1/Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG, Scer\GAL4GMR.PU is a suppressor of eye phenotype of FIG4KK102943, Scer\GAL4GMR.PU
fruTOE.GS02668/Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG, Scer\GAL4Or47b.7.467 is a suppressor of adult olfactory receptor neuron Or47b | adult stage | conditional phenotype of MetKK104562, Scer\GAL4Or47b.7.467
fruTOE.GS02668, Spyo\Cas9dCas9::VPR.UAS.Tag:FLAG, Scer\GAL4Or47b.7.467 is a suppressor of adult olfactory receptor neuron Or47b | adult stage | conditional phenotype of Scer\GAL4Or47b.7.467, nejRNAi.UAS.cSa