FB2024_03 , released June 25, 2024
Allele: Dmel\TFAMUAS.cCa
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General Information
Symbol
Dmel\TFAMUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Cagin
FlyBase ID
FBal0324154
Feature type
allele
Associated gene
Dmel\TFAM
Associated Insertion(s)
Carried in Construct
P{UAS-TFAM.C}
Also Known As
UAS-TFAM
Key Links
Transgenic product class
cDNA
(Cagin et al., 2015)
wild_type
(Cagin et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Cagin et al., 2015)
wild_type
(Cagin et al., 2015)
Mutagen
in vitro construct
(Cagin et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-TFAM.C}
Cytology
Description

UASt regulatory sequences drive expression of TFAM (sequence derived from the LD40493 cDNA clone).

(Cagin et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
TFAM
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal circadian behavior | adult stage, with Scer\GAL4tim.PE

      (Chen et al., 2019)

      abnormal circadian behavior | adult stage, with Scer\GAL4tim.PE, Scer\GAL80Pdf.PS

      (Chen et al., 2019)

      abnormal locomotor behavior | adult stage, with Scer\GAL4Toll-6-D42

      (Cagin et al., 2015)

      abnormal locomotor behavior | adult stage, with Scer\GAL4VGlut-OK371

      (Cagin et al., 2015)

      abnormal locomotor behavior | adult stage | male, with Scer\GAL4nSyb.PS, TFAMc01716

      (Hunt et al., 2019)

      abnormal locomotor behavior | adult stage | male, with Scer\GAL4Toll-6-D42

      (Hunt et al., 2019)

      abnormal locomotor rhythm | adult stage, with Scer\GAL4tim.PE

      (Chen et al., 2019)

      abnormal locomotor rhythm | adult stage, with Scer\GAL4tim.PE, Scer\GAL80Pdf.PS

      (Chen et al., 2019)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4109(2)80

      (Tsuyama et al., 2017)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4VGlut-OK371

      (Cagin et al., 2015)

      lethal - all die before end of P-stage, with Scer\GAL4nSyb.PU

      (Cagin et al., 2015)

      lethal - all die before end of P-stage, with Scer\GAL4VGlut-OK371

      (Cagin et al., 2015)

      lethal - all die during first instar larval stage, with Scer\GAL4da.PU

      (Cagin et al., 2015)

      majority die during pupal stage, with Scer\GAL4nSyb.PS

      (Hunt et al., 2019)

      short lived, with Scer\GAL4Toll-6-D42

      (Cagin et al., 2015)

      short lived, with Scer\GAL4VGlut-OK371

      (Cagin et al., 2015)

      some die during P-stage, with Scer\GAL4VGlut-OK371

      (Cagin et al., 2015)

      visible | adult stage, with Scer\GAL4nSyb.PS, TFAMc01716

      (Hunt et al., 2019)

      visible | adult stage, with Scer\GAL4Toll-6-D42

      (Hunt et al., 2019, Cagin et al., 2015)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of TFAMUAS.cCa under the control of Scer\GAL4tim.PE (alone or in combination with either Scer\GAL80Pdf.PS or tub-Gal80[ts], to restrict expression to the adult stage) but not Scer\GAL4Pdf.PU, leads to a significant reduction in the amplitude of locomotor activity rhythm (circadian rhythm) under constant darkness when compared to control adults.

      (Chen et al., 2019)

      Expression of TFAMUAS.cCa under the control of Scer\GAL4Toll-6-D42 leads to climbing and wing inflation defects when compared to controls.

      Expression of TFAMUAS.cCa under the control of Scer\GAL4nSyb.PS leads to almost complete late pupal lethality.

      Expression of TFAMUAS.cCa under the control of Scer\GAL4nSyb.PS in the TFAMc01716/+ heterozygous mutant background leads to climbing and wing inflation defects when compared to controls.

      (Hunt et al., 2019)

      Expression of TFAMScer\UAS.cCa under the control of Scer\GAL4109(2)80 results in slight shortening of the dendritic arbor in the class IV ddaC neurons in third instar larvae.

      (Tsuyama et al., 2017)

      Expression of TFAMScer\UAS.cCa under the control of Scer\GAL4da.PU is first instar larval lethal; expression under the Scer\GAL4VGlut-OK371 driver at 25[o]C is partially pupal lethal, the surviving adults do not live beyond a week and display severe climbing ability defects, at 29[o]C the expression is fully pupal lethal. Neither the number of motoneuron cell bodies nor the level of cell death in the ventral nerve cord is significantly affected in third instar larvae. However, the number of active zones in the neuromuscular junctions (NMJs) is significantly decreased (while the number of boutons is not changed) and the number and volume of presynaptic mitochondria at the NMJ is also strongly reduced relative to controls, although their ultrastructure appear normal. TFAMScer\UAS.cCa expression affects mitochondria in other parts of the motor neuron as well: the cell body mitochondria are less reticular with more punctate morphology and both the number and volume of mitochondria in the distal axon is reduced, no significant effect is observed in the proximal axon.

      Expression under the Scer\GAL4Toll-6-D42 also reduces adult climbing ability and lifespan and leads to failed wing inflation in about 50% of the adult flies.

      (Cagin et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4nSyb.PS, TFAMUAS.cCa, TFAMc01716 has visible | adult stage phenotype, suppressible by L2HGDHUAS.cLa, Scer\GAL4nSyb.PS

      (Hunt et al., 2019)

      Scer\GAL4Toll-6-D42, TFAMUAS.cCa has abnormal locomotor behavior | male | adult stage phenotype, suppressible by Scer\GAL4Toll-6-D42/crcJF02007

      (Hunt et al., 2019)

      Scer\GAL4Toll-6-D42, TFAMUAS.cCa has visible | adult stage phenotype, suppressible by Scer\GAL4Toll-6-D42/crcJF02007

      (Hunt et al., 2019)

      Scer\GAL4nSyb.PS, TFAMUAS.cCa has majority die during pupal stage phenotype, suppressible | partially by Scer\GAL4nSyb.PS/crcJF02007

      (Hunt et al., 2019)

      Scer\GAL4nSyb.PS, TFAMUAS.cCa, TFAMc01716 has abnormal locomotor behavior | male | adult stage phenotype, suppressible by L2HGDHUAS.cLa, Scer\GAL4nSyb.PS

      (Hunt et al., 2019)

      Scer\GAL4109(2)80, TFAMUAS.cCa has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by PPP1R15UAS.cMa, Scer\GAL4109(2)80

      (Tsuyama et al., 2017)

      Scer\GAL4Toll-6-D42, TFAMUAS.cCa has abnormal locomotor behavior | adult stage phenotype, suppressible by simaHMS00833/Scer\GAL4Toll-6-D42

      (Cagin et al., 2015)

      Scer\GAL4Toll-6-D42, TFAMUAS.cCa has abnormal locomotor behavior | adult stage phenotype, suppressible by Scer\GAL4Toll-6-D42/simaHMS00832

      (Cagin et al., 2015)

      Scer\GAL4Toll-6-D42, TFAMUAS.cCa has visible | adult stage phenotype, suppressible by simaHMS00833/Scer\GAL4Toll-6-D42

      (Cagin et al., 2015)

      Scer\GAL4Toll-6-D42, TFAMUAS.cCa has visible | adult stage phenotype, suppressible by Scer\GAL4Toll-6-D42/simaHMS00832

      (Cagin et al., 2015)

      Scer\GAL4Toll-6-D42, TFAMUAS.cCa has short lived phenotype, suppressible by simaHMS00833/Scer\GAL4Toll-6-D42

      (Cagin et al., 2015)

      Scer\GAL4Toll-6-D42, TFAMUAS.cCa has short lived phenotype, suppressible by Scer\GAL4Toll-6-D42/simaHMS00832

      (Cagin et al., 2015)

      Scer\GAL4VGlut-OK371, TFAMUAS.cCa has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4VGlut-OK371/simaHMS00833

      (Cagin et al., 2015)
      NOT suppressed by
      NOT Enhancer of
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of crcJF02007 under the control of Scer\GAL4Toll-6-D42 alleviates climbing and wing inflation defects exhibited as a result of expression of TFAMUAS.cCa under the control of Scer\GAL4Toll-6-D42.

      Expression of crcJF02007 under the control of Scer\GAL4nSyb.PS alleviates almost complete late pupal lethality exhibited as a result of expression of TFAMUAS.cCa under the control of Scer\GAL4nSyb.PS.

      Expression of L2HGDHHMC03444 under the control of Scer\GAL4nSyb.PS exacerbates climbing and wing inflation defects exhibited as a result of expression of TFAMUAS.cCa under the control of Scer\GAL4nSyb.PS in the TFAMc01716/+ heterozygous mutant background.

      Expression of L2HGDHUAS.cLa under the control of Scer\GAL4nSyb.PS suppresses climbing and wing inflation defects exhibited as a result of expression of TFAMUAS.cCa under the control of Scer\GAL4nSyb.PS in the TFAMc01716/+ heterozygous mutant background.

      (Hunt et al., 2019)

      The shortening of the dendritic arbor in class IV ddaC neurons observed in third instar larvae expressing TFAMScer\UAS.cCa under the control of Scer\GAL4109(2)80 can be rescued by co-expression of PPP1R15Scer\UAS.cMa, although occasionally the neurons display a severe dendrite loss instead.

      (Tsuyama et al., 2017)

      The reduced climbing ability characteristic for flies expressing either TFAMScer\UAS.cCa or simaScer\UAS.cBa alone under the control of Scer\GAL4Toll-6-D42 is not decreased further when the two transgenes are co-expressed. However, the adult locomotion and wing inflation defects of TFAMScer\UAS.cCa expressing flies as well as their reduced lifespan are significantly improved by co-expression of either simaHMS00833 or simaHMS00832. The loss of mitochondria at the larval neuromuscular junction induced by Scer\GAL4VGlut-OK371-driven expression of TFAMScer\UAS.cCa is not affected by co-expression of simaHMS00833, while the reduction in the number of active zones at the NMJ is fully rescued.

      (Cagin et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TFAMScer\UAS.cCa
      TFAMUAS.cCa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Cagin
      Secondary FlyBase IDs
        References (4)