The expression of VincHead.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP under the control of either Scer\GAL4Act5C.PU or Scer\GAL4Mef2.PU leads to lethality; expression under the control of Scer\GAL4Mef2.PU also leads to a range of embryonic muscle defects in the majority of cases, including misshapen or misplaced muscles, gaps in the muscle pattern, and ectopic attachments, and to the formation of ectopic VincHead.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP-positive cytoplasmic protein aggregates in those muscles, as compared to controls; clonal expression under the control of Scer\GAL4Act5C.PU also leads to the formation of ectopic VincHead.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP-positive cytoplasmic protein aggregates in the follicular and pupal wing epithelia, as compared to controls.
Scer\GAL4Mef2.PU, VincHead.UASp.TagRFP has lethal phenotype, suppressible by rheaIBS2.UAS.mGFP6/Scer\GAL4Mef2.PU
Scer\GAL4Mef2.PU, VincHead.UASp.TagRFP has lethal phenotype, suppressible by rheaVBS1.UASp.GFP/Scer\GAL4Mef2.PU
Scer\GAL4Mef2.PU, VincHead.UASp.TagRFP has lethal phenotype, suppressible by Scer\GAL4Mef2.PU/rheaVBS2.UASp.GFP
Scer\GAL4Act5C.PU, VincHead.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible | partially by rheaB28
Scer\GAL4Mef2.PU, VincHead.UASp.TagRFP has embryonic somatic muscle cell phenotype, suppressible | partially by rheaIBS2.UAS.mGFP6/Scer\GAL4Mef2.PU
The lethality induced by the expression of VincHead.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP under the control of Scer\GAL4Mef2.PU is suppressed by co-expressing either rheaVBS1.Scer\UAS.P\T.T:Avic\GFP, rheaVBS2.Scer\UAS.P\T.T:Avic\GFP, or rheaIBS2.Scer\UAS.T:Avic\GFP-m6.
The number of ectopic VincHead.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP-positive cytoplasmic protein aggregates formed upon the expression of VincHead.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP in follicle cell clones (driven by Scer\GAL4Act5C.PU) and in embryonic muscles (driven by Scer\GAL4Mef2.PU) is strongly reduced by rheaB28 homozygosity or co-expression of rheaIBS2.Scer\UAS.T:Avic\GFP-m6, respectively.