FlyBase Allele Report: Dmel\rhea[B28]

General Information

Symbol

Dmel\rhea^B28

Species

D. melanogaster

Name

FlyBase ID

FBal0304153

Feature type

allele

Associated gene

Dmel\rhea

Associated Insertion(s)

Carried in Construct

Also Known As

talin⁶²

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Klapholz et al., 2015)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A.

(Klapholz et al., 2015)

Amino acid replacement: W63term.

(Klapholz et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:8,561,739..8,561,739 [+]

Nucleotide change:

G8561739A

Reported nucleotide change:

G?A

Amino acid change:

Reported amino acid change:

W63term

Comment:

Nonsense mutation (TGG to TGA) at Trp codon.

(Klapholz et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion | recessive

(Klapholz et al., 2015)

abnormal cell adhesion | recessive | somatic clone

(Klapholz et al., 2015)

visible | recessive | somatic clone

(Klapholz et al., 2015)

Phenotype Manifest In

muscle attachment site

(Klapholz et al., 2015)

wing | somatic clone

(Klapholz et al., 2015)

Detailed Description

Statement

Reference

Homozygous clones in the wing result in wing blisters in more than 95% of wings.

Homozygous embryos show complete muscle detachment (quantified as greater than 40% shortening of the dorsal muscles).

More than 25% of homozygotes fail to undergo germband retraction (this phenotype is indistinguishable from that of a null mutation).

(Klapholz et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

rhea^B28 is a suppressor of follicle cell | somatic clone phenotype of Scer\GAL4^Act5C.PU, Vinc^{CO.UASp.TagRFP}

(Maartens et al., 2016)

rhea^B28 is a suppressor | partially of follicle cell | somatic clone phenotype of Scer\GAL4^Act5C.PU, Vinc^{Head.UASp.TagRFP}

(Maartens et al., 2016)

rhea^B28 is a suppressor | partially of follicle cell | somatic clone phenotype of Scer\GAL4^Act5C.PU, Vinc^{D1.UASp.TagRFP}

(Maartens et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

rhea^B28 homozygosity nearly abrogates the ectopic Vinc^{CO.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP}-positive cytoplasmic protein aggregates formed in follicle cell clones and embryonic muscles, upon expression under the control of Scer\GAL4^Act5C.PU and Scer\GAL4^Mef2.PU, respectively.

(Maartens et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600183

w^*; P{GFP-talin.wt}J1/CyO; rhea^B28 Fit2⁸³ P{FRT(w^hs)}2A/TM6B, P{Dfd-EYFP}3, Sb¹ Tb¹ ca¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

rhea⁶²

(Green et al., 2018)

rhea^B28

(Chen et al., 2018)

talin⁶²

(Maartens et al., 2016, Klapholz et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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