The expression of VincD1.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP under the control of either Scer\GAL4Act5C.PU or Scer\GAL4Mef2.PU leads to lethality; expression under the control of Scer\GAL4Mef2.PU also leads to embryonic muscle defects in a small proportion of cases, and to the formation of ectopic VincD1.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP-positive cytoplasmic protein aggregates in embryonic muscles, as compared to controls.
Scer\GAL4Mef2.PU, VincD1.UASp.TagRFP has lethal phenotype, suppressible by rheaIBS2.UAS.mGFP6/Scer\GAL4Mef2.PU
Scer\GAL4Mef2.PU, VincD1.UASp.TagRFP has lethal phenotype, suppressible by rheaVBS1.UASp.GFP/Scer\GAL4Mef2.PU
Scer\GAL4Mef2.PU, VincD1.UASp.TagRFP has lethal phenotype, suppressible by Scer\GAL4Mef2.PU/rheaVBS2.UASp.GFP
Scer\GAL4Act5C.PU, VincD1.UASp.TagRFP has follicle cell | somatic clone phenotype, suppressible | partially by rheaB28
Scer\GAL4Mef2.PU, VincD1.UASp.TagRFP has embryonic somatic muscle cell phenotype, suppressible | partially by rheaIBS2.UAS.mGFP6/Scer\GAL4Mef2.PU
The lethality induced by the expression of VincD1.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP under the control of Scer\GAL4Mef2.PU is suppressed by the co-expression of either rheaVBS1.Scer\UAS.P\T.T:Avic\GFP, rheaVBS2.Scer\UAS.P\T.T:Avic\GFP, or rheaIBS2.Scer\UAS.T:Avic\GFP-m6.
The number of ectopic VincD1.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP-positive cytoplasmic protein aggregates formed upon the expression of VincD1.Scer\UAS.P\T.T:Equa\eqFP578-TagRFP in follicle cell clones (driven by Scer\GAL4Act5C.PU) and in embryonic muscles (driven by Scer\GAL4Mef2.PU) is strongly reduced by rheaB28 homozygosity and co-expression of rheaIBS2.Scer\UAS.T:Avic\GFP-m6, respectively.