FlyBase Allele Report: Dmel\tsr[RNAi.UAS]

General Information

Symbol

Dmel\tsr^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0325830

Feature type

allele

Associated gene

Dmel\tsr

Associated Insertion(s)

Carried in Construct

P{UAS-tsr.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Ko et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ko et al., 2016)

Mutagen

in vitro construct

(Ko et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-tsr.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of tsr inverted sequences (-147 to 388 bp of the second exon).

(Ko et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tsr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing basal cell 1 | adult stage, with Scer\GAL4^ptc.PU

(Ko et al., 2016)

wing disc | third instar larval stage, with Scer\GAL4^ptc.PU

(Ko et al., 2016)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^en.PU

(Ko et al., 2016)

Detailed Description

Statement

Reference

The expression of tsr^dsRNA.UAS under the control of Scer\GAL4^en.PU leads to a significant decrease in the proportion of cells in S-phase (BrdU-positive cells), but not in mitosis (phospho-H3 immunofluorescence), within the posterior compartment of the third instar wing disc cells, as compared to controls; this expression also leads to an obvious increase in apoptosis (both TUNEL assays and MMP1 immunofluorescence) within the compartment, as compared to controls.

The expression under the control of Scer\GAL4^ptc.PU leads to the dispersion of the expression cells, resulting in irregular edges along the ptc-expressing domain, as well as in many cells detached from the domain, and leads to frequent basal extrusion, as compared to controls; this expression also leads to an obvious increase in apoptosis (Casp3 immunofluorescence), but not in mitosis (phospho-H3 immunofluorescence), within the ptc-domain, as compared to controls. The resulting adult wing exhibits tissue reduction in the ptc domain between veins 3 and 4, associated with a disrupted local wing cell pattern, as compared to controls.

(Ko et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ptc.PU, tsr^RNAi.UAS has abnormal cell adhesion | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cUa, Scer\GAL4^ptc.PU

(Ko et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc.PU, tsr^RNAi.UAS has increased cell death | third instar larval stage phenotype, non-suppressible by msn^HMJ02084/Scer\GAL4^ptc.PU

(Ko et al., 2016)

Other

Statement

Reference

BacA\p35^UAS.cUa, Scer\GAL4^ptc.PU, tsr^RNAi.UAS has increased occurrence of cell division | third instar larval stage phenotype

(Ko et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ptc.PU, tsr^RNAi.UAS has wing disc | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cUa, Scer\GAL4^ptc.PU

(Ko et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc.PU, tsr^RNAi.UAS has wing disc | third instar larval stage phenotype, non-suppressible by msn^HMJ02084/Scer\GAL4^ptc.PU

(Ko et al., 2016)

Other

Statement

Reference

BacA\p35^UAS.cUa, Scer\GAL4^ptc.PU, tsr^RNAi.UAS has wing disc | third instar larval stage phenotype

(Ko et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased apoptosis and cell dispersion within the ptc-domain of the third instar larval wing disc induced by the expression of tsr^dsRNA.UAS under the control of Scer\GAL4^ptc.PU are not suppressed by the co-expression of msn^HMJ02084.

(Ko et al., 2016)

Xenogenetic Interactions

Statement

Reference

The dispersion of cells from the ptc-expressing domain of the third instar larval wing disc induced by the expression of tsr^dsRNA.UAS under the control of Scer\GAL4^ptc.PU is enhanced by the co-expression of BacA\p35^UAS.cUa, even leading to the enlargement of the ptc-domain stripe.

The co-expression of tsr^dsRNA.UAS and BacA\p35^UAS.cUa under the control of Scer\GAL4^ptc.PU leads to a significantly increased mitotic index (phospho-H3 immunofluorescence) in the ptc-expressing domain within the third instar larval wing disc, as compared to controls.

(Ko et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

tsr^RNAi.UAS

tsr^{dsRNA.Scer\UAS}

tsr^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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