UASt regulatory sequences drive expression of α-Cat that lacks amino acid residues 265-397. The protein is tagged with Tag:V5. Synonymous mutations have been introduced into the portion of the α-Cat coding region that corresponds to the target sequence of the P{TRiP.HMS00317} RNAi line, rendering this transgene insensitive to RNAi mediated by P{TRiP.HMS00317}.
α-CatΔM1a.UAS.Tag:V5/Scer\GAL4NP7397 fails to rescue α-CatHMS00317