FB2024_03 , released June 25, 2024
Allele: Dmel\α-CatH1-ΔβH.UASp.R.Tag:HA
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General Information
Symbol
Dmel\α-CatH1-ΔβH.UASp.R.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0359819
Feature type
allele
Associated gene
Dmel\α-Cat
Associated Insertion(s)
Carried in Construct
P{UASp-α-Cat.R.H1-ΔβH.HA}
Key Links
Transgenic product class
inframe_deletion
(Sarpal et al., 2019)
missense_variant
(Sarpal et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Sarpal et al., 2019)
missense_variant
(Sarpal et al., 2019)
Mutagen
in vitro construct
(Sarpal et al., 2019)
Progenitor genotype
Carried in construct
P{UASp-α-Cat.R.H1-ΔβH.HA}
Cytology
Description

UASp regulatory sequences drive expression of α-Cat that has two mutations within the actin-binding domain; the α1-helix has a REAM to GSGS change starting at residue 683 and the β-sheet hairpin loop is deleted (amino acid residues 812-823 are absent). The coding sequence is tagged at the C-terminal end with Tag:HA. The α-Cat sequence has been modified to render it resistant to RNAi by either P{UAS-α-Cat.RNAi.1} (5'-ATGTTAAAA-3' changed to 5'-ATGCTGAAG-3' at the start of the coding region) or P{TRiP.HMS00317} (5'-GCAGCATCGATATTGACTGTT-3' changed to 5'-GCCGCCTCCATCCTGACCGTG-3' in the M2 domain).

(Sarpal et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
α-Cat
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      α-CatH1-ΔβH.UASp.R.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)