FB2024_03 , released June 25, 2024
Recombinant Construct: P{UASp-α-Cat.R.H1-ΔβH.HA}
Open Close
General Information
Symbol
P{UASp-α-Cat.R.H1-ΔβH.HA}
FlyBase ID
FBtp0141967
Feature type
transgenic_transposable_element
Component Allele(s)
α-CatH1-ΔβH.UASp.R.Tag:HA
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele α-CatH1-ΔβH.UASp.R.Tag:HA
Transgenic product class
inframe_deletion
(Sarpal et al., 2019)
missense_variant
(Sarpal et al., 2019)
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
α-Cat
Tagged with
Tag:HA
Description

UASp regulatory sequences drive expression of α-Cat that has two mutations within the actin-binding domain; the α1-helix has a REAM to GSGS change starting at residue 683 and the β-sheet hairpin loop is deleted (amino acid residues 812-823 are absent). The coding sequence is tagged at the C-terminal end with Tag:HA. The α-Cat sequence has been modified to render it resistant to RNAi by either P{UAS-α-Cat.RNAi.1} (5'-ATGTTAAAA-3' changed to 5'-ATGCTGAAG-3' at the start of the coding region) or P{TRiP.HMS00317} (5'-GCAGCATCGATATTGACTGTT-3' changed to 5'-GCCGCCTCCATCCTGACCGTG-3' in the M2 domain).

(Sarpal et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UASp-α-Cat.R.H1-ΔβH.HA}
(FlyBase, 1992-)
UAS-α-CatR-H1-ΔβH
(Sarpal et al., 2019)
Secondary FlyBase IDs
    References (2)