FlyBase Gene Report: Dmel\H15

General Information

Symbol

Dmel\H15

Species

D. melanogaster

Name

H15

Annotation Symbol

CG6604

Feature Type

protein_coding_gene

FlyBase ID

FBgn0016660

Gene Model Status

Current

Stock Availability

46 publicly available

Gene Summary

H15 (H15) encodes a T-box transcription factor that regulates the specification of cell fate in the heart and central nervous system. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

nmr, nmr1, neuromancer, neuromancer1, neuromancer 1

Key Links

Genomic Location

Cytogenetic map

25E1-25E1

Sequence location

2L:5,404,280..5,416,593 [+]

Recombination map

2-17

RefSeq locus

NT_033779 REGION:5404280..5416593

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN004251338

(GO Reference Genome Project, 2011-)

enables DNA-binding transcription factor activity

inferred from electronic annotation with InterPro:IPR001699, InterPro:IPR002070, InterPro:IPR008967, InterPro:IPR018186, InterPro:IPR036960, InterPro:IPR046360

(InterPro Project Members, 2004-)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN004251338

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001699

(InterPro Project Members, 2004-)

Biological Process (11 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in cardioblast cell fate commitment

inferred from mutant phenotype

(Qian et al., 2005)

involved_in chorion-containing eggshell pattern formation

inferred from mutant phenotype

(Fregoso Lomas et al., 2013)

involved_in embryonic heart tube development

inferred from mutant phenotype

(Qian et al., 2005)

involved_in heart development

inferred from mutant phenotype

(Miskolczi-McCallum et al., 2005)

involved_in heart process

inferred from direct assay

(Qian et al., 2008)

involved_in leg disc pattern formation

inferred from genetic interaction with FLYBASE:mid; FB:FBgn0261963

(Svendsen et al., 2009)

involved_in positive regulation of transcription by RNA polymerase II

inferred from direct assay

(Gaziova and Bhat, 2009)

involved_in regulation of cell fate specification

inferred from genetic interaction with FLYBASE:mid; FB:FBgn0261963

(Svendsen et al., 2009)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in cell fate specification

inferred from biological aspect of ancestor with PANTHER:PTN000137774

(GO Reference Genome Project, 2011-)

involved_in heart development

inferred from biological aspect of ancestor with PANTHER:PTN002601330

(GO Reference Genome Project, 2011-)

involved_in positive regulation of DNA-templated transcription

inferred from electronic annotation with InterPro:IPR046360

(InterPro Project Members, 2004-)

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN004251338

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of chromatin

inferred from biological aspect of ancestor with PANTHER:PTN004251338

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN004251338

(GO Reference Genome Project, 2011-)

located_in nucleus

inferred from electronic annotation with InterPro:IPR002070, InterPro:IPR018186, InterPro:IPR036960

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

T-BOX TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

H15 (H15) encodes a T-box transcription factor that regulates the specification of cell fate in the heart and central nervous system. [Date last reviewed: 2019-07-11]

Gene Group (FlyBase)

T-BOX TRANSCRIPTION FACTORS -: The T-box transcription factors are sequence-specific DNA binding proteins that regulate transcription. They are characterized by a conserved DNA-binding immunoglobulin-fold domain of 180-200 amino acids, known as T-box, named after its founding member, the mouse T protein. (Adapted from FBrf0218841).

Summary (Interactive Fly)

T-box transcription factors that participate in cardiac fate specificity - regulates wing development by repressing wingless and hedgehog

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\H15 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q94890)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

H15-RA

FBtr0079076

NM_135082

2617

660

H15-RB

FBtr0343225

NM_001298698

2926

659

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

H15-PA

FBpp0078710

72.4

660

8.94

FBpp0309914

72.2

659

8.94

X2J9F2

NP_001285627

AHN54142

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\H15 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

H15_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.52

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 15

dorsal vessel primordium

(Qian et al., 2005)

embryonic stage 13 -- 16

larval foregut atrium

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic/larval dorsal vessel

(Fisher et al., 2012)

embryonic stage 16 -- 17

embryonic dorsal vessel

(Qian et al., 2005)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult heart

(Qian et al., 2008)

Additional Descriptive Data

H15 transcript is expressed in the developing dorsal vessel from embryonic stage 13.

(Qian et al., 2005)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

neuroblast NB2-3

(Lacin and Truman, 2016)

neuroblast NB6-1

(Lacin and Truman, 2016)

larval stage

neuroblast NB2-3

(Lacin and Truman, 2016)

neuroblast NB6-1

(Lacin and Truman, 2016)

wandering third instar larval stage -- prepupal stage P2

photoreceptor neuron

(Das et al., 2013)

adult stage

adult ostium

(Tang et al., 2013)

cardioblast

(Tang et al., 2013)

oogenesis stage S8 -- S10B & adult stage | female

main body follicle cell | posterior | subset

• nucleus

(Fregoso Lomas et al., 2013)

Additional Descriptive Data

H15 is expressed in oocyte-associated follicle cells covering roughly the posterior end of the egg chamber at stage 10B. Length-wise, the domain of H15 expression goes from the posterior tip of the egg chamber halfway towards where the dorsal appendage forming follicle cells begin.

(Fregoso Lomas et al., 2013)

Anti-H15 staining identifies small ostial

(Tang et al., 2013)

cell nuclei and larger myocardial cell (cardioblast) nuclei.

(Tang et al., 2013)

Marker for

adult ostium

(Tang et al., 2013)

cardioblast

(Tang et al., 2013)

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{PZ}H-15

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12 -- 15

dorsal vessel primordium

(Griffin et al., 2000)

embryonic head

(Griffin et al., 2000)

embryonic/larval central nervous system | segmentally repeated | striped | ventral

(Griffin et al., 2000)

embryonic/larval peripheral nervous system

(Griffin et al., 2000)

epidermis | anterior | segmentally repeated | ventral

(Griffin et al., 2000)

embryonic stage 12 -- 17

embryonic peripheral glial cell 3

(von Hilchen et al., 2008)

embryonic peripheral glial cell 4

(von Hilchen et al., 2008)

embryonic peripheral glial cell 7

(von Hilchen et al., 2008)

embryonic peripheral glial cell 11

(von Hilchen et al., 2008)

third instar larval stage

imaginal disc | restricted

(Wilder and Perrimon, 1995)

imaginal disc | ventral

(Wilder and Perrimon, 1995)

adult stage

cardial cell

(Qian et al., 2008)

adult ostium

(Qian et al., 2008)

adult heart

(Qian et al., 2008)

oogenesis stage S8 -- S10B & adult stage | female

main body follicle cell | posterior | subset

• nucleus

(Fregoso Lomas et al., 2013)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\H15 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\H15

View exonic expression by tissue for Dmel\H15

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016660

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33769/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016660

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016660

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016660

Flygut - An atlas of the Drosophila adult midgut

FBgn0016660

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of H15

Transgenic constructs containing regulatory region of H15

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

Df(2L)Exel6012

(Ryder, 2004.4.26)

Df(2L)Gpdh1-A

(Miskolczi-McCallum et al., 2005, Buescher et al., 2004)

Df(2L)H15-x4

(Miskolczi-McCallum et al., 2005)

Df(2L)sc19-4

(Leal et al., 2009)

Df(2L)x528

(Leal et al., 2009, Miskolczi-McCallum et al., 2005, Buescher et al., 2004)

Gene partially disrupted in

Df(2L)ΔG04

(Stevens et al., 2022)

Df(2L)ΔG04-F11

(Stevens et al., 2022)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

H15^GD12818

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

H15^GD12818

viable

viable, with Scer\GAL4^Mef2.PR

H15^GD12818

Sterility

Allele

fertile

H15^nmr-210

Other Phenotypes

Allele

abnormal adult locomotory behavior, with Scer\GAL4^Mef2.PR

H15^GD12818

abnormal flight, with Scer\GAL4^Mef2.PR

H15^KK110915

abnormal jumping, with Scer\GAL4^Mef2.PR

H15^GD12818

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

H15^GD12818

decreased size | adult stage, with Scer\GAL4^Mef2.PR

H15^GD12818

flightless, with Scer\GAL4^Mef2.PR

H15^GD12818

some die during pupal stage, with Scer\GAL4^pnr-MD237

Phenotype manifest in

Allele

dorsal appendage | somatic clone, with Scer\GAL4^αTub84B.PL

H15^UAS.cQa

flight muscle | adult stage, with Scer\GAL4^Mef2.PR

H15^GD12818

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

H15^GD12818

indirect flight muscle | adult stage, with Scer\GAL4^Mef2.PR

H15^GD12818

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

H15^GD12818

mitochondrion | adult stage, with Scer\GAL4^Mef2.PR

H15^GD12818

myotube | adult stage, with Scer\GAL4^Mef2.PR

H15^GD12818

sarcomere | adult stage, with Scer\GAL4^Mef2.PR

H15^GD12818

skeletal muscle myofibril | adult stage, with Scer\GAL4^Mef2.PR

H15^GD12818

somatic muscle of leg | adult stage, with Scer\GAL4^Mef2.PR

H15^GD12818

tergotrochanter muscle | adult stage, with Scer\GAL4^Mef2.PR

H15^GD12818

tergotrochanter muscle cell, with Scer\GAL4^Mef2.PR

H15^GD12818

Z disc | adult stage, with Scer\GAL4^Mef2.PR

H15^GD12818

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (17)

Hsap\TBX20

9 of 14

Yes

Hsap\MGA

4 of 14

Yes

Hsap\TBX19

4 of 14

Hsap\TBXT

4 of 14

Hsap\EOMES

3 of 14

Yes

Hsap\TBR1

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Hsap\TBX18

3 of 14

Hsap\TBX21

3 of 14

Hsap\TBX22

3 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (17)

Rnor\Tbx20

10 of 14

Yes

Rnor\Mga

4 of 14

Yes

Rnor\Tbx15

4 of 14

Yes

Rnor\Tbx22

4 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Rnor\Tbx5

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (17)

Mmus\Tbx20

9 of 14

Yes

Mmus\Mga

4 of 14

Yes

Mmus\Tbx15

4 of 14

Yes

Mmus\Tbx22

4 of 14

Yes

Mmus\T

4 of 14

Mmus\Eomes

3 of 14

Yes

Mmus\Tbr1

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Mmus\Tbx5

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Xenopus tropicalis (Western clawed frog) (23)

Xtro\tbx20

6 of 13

Yes

Xtro\mga

2 of 13

Xtro\tbx15

2 of 13

Yes

Xtro\tbx18

2 of 13

Yes

Xtro\tbx22

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\plch1

1 of 13

Xtro\prnp

1 of 13

Yes

Xtro\retsat

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (25)

Drer\tbx20

8 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

Yes

Drer\mgaa

3 of 14

Yes

Drer\tbr1a

3 of 14

Yes

Drer\tbr1b

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Drer\tbx5a

3 of 14

Yes

Drer\tbx6

3 of 14

Drer\tbx15

3 of 14

Yes

Drer\tbx16

3 of 14

Drer\tbx21

3 of 14

Yes

Drer\tbxta

3 of 14

Drer\eomesb

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (22)

Cele\mab-9

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Cele\tbx-42

2 of 14

Cele\tbx-43

2 of 14

Anopheles gambiae (African malaria mosquito) (12)

Agam\AgaP_AGAP008569

7 of 12

Yes

Agam\AgaP_AGAP008563

5 of 12

Agam\AgaP_AGAP008567

5 of 12

Yes

4 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

1 of 12

Yes

Agam\AgaP_AGAP010686

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:H15. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

mid

10 of 13

org-1

5 of 13

4 of 13

byn

4 of 13

Doc1

4 of 13

Doc2

4 of 13

Doc3

4 of 13

ocm

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

atrial septal defect 4

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

H15^nmr-614

model of cardiomyopathy

CEA

(Qian et al., 2008)

model of congenital heart disease

CEA

(Qian et al., 2008)

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

TBX20; T-box transcription factor 20

model of atrial heart septal defect 4

IEA

(FlyBase, 2019-)

TBX22; T-box transcription factor 22

model of X-linked cleft palate with or without ankyloglossia

IEA

(FlyBase, 2019-)

model of Abruzzo-Erickson syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TBX20; T-box transcription factor 20

9 of 14

606061

ATRIAL SEPTAL DEFECT 4; ASD4

atrial heart septal defect 4

MGA; MAX dimerization protein MGA

4 of 14

616061

TBX19; T-box transcription factor 19

4 of 14

604614

ACTH DEFICIENCY, ISOLATED; IAD

adrenocorticotropic hormone deficiency

TBXT; T-box transcription factor T

4 of 14

601397

EOMES; eomesodermin

3 of 14

604615

TBR1; T-box brain transcription factor 1

3 of 14

604616

INTELLECTUAL DEVELOPMENTAL DISORDER WITH AUTISM AND SPEECH DELAY; IDDAS

TBX1; T-box transcription factor 1

3 of 14

602054

TBX2; T-box transcription factor 2

3 of 14

600747

VERTEBRAL ANOMALIES AND VARIABLE ENDOCRINE AND T-CELL DYSFUNCTION; VETD

vertebral anomalies and variable endocrine and T-cell dysfunction

TBX3; T-box transcription factor 3

3 of 14

601621

ULNAR-MAMMARY SYNDROME; UMS

ulnar-mammary syndrome

TBX4; T-box transcription factor 4

3 of 14

601719

ischiocoxopodopatellar syndrome

TBX5; T-box transcription factor 5

3 of 14

601620

HOLT-ORAM SYNDROME; HOS

Holt-Oram syndrome

TBX6; T-box transcription factor 6

3 of 14

602427

SPONDYLOCOSTAL DYSOSTOSIS 5; SCDO5

spondylocostal dysostosis 5

TBX10; T-box transcription factor 10

3 of 14

604648

TBX15; T-box transcription factor 15

3 of 14

604127

COUSIN SYNDROME

TBX18; T-box transcription factor 18

3 of 14

604613

CONGENITAL ANOMALIES OF KIDNEY AND URINARY TRACT 2; CAKUT2

CAKUT2

TBX21; T-box transcription factor 21

3 of 14

604895

asthma, nasal polyps, and aspirin intolerance

TBX22; T-box transcription factor 22

3 of 14

300307

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

H15 - Hr78

two hybrid array

(Shokri et al., 2019)

H15 - Set1

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

H15 - atms

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

H15 - mid

two hybrid array

(Shokri et al., 2019)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

mid

enhanceable

H15

(Qian et al., 2005)

pnr

suppressible

H15

(Qian and Bodmer, 2009)

salm

suppressible

H15

(Katti et al., 2022)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0016660

MIST (genetic) - An integrated Molecular Interaction Database

33769

MIST (protein-protein) - An integrated Molecular Interaction Database

33769

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-17

Cytogenetic map

25E1-25E1

Sequence location

2L:5,404,280..5,416,593 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

25E1-25E1

Limits computationally determined from genome sequence between P{lacW}vri^k05901&P{PZ}cype⁰³⁷⁷¹ and P{lacW}Hel25E^k11511

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-17

(FlyBase, 2016)

2-18.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (46)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

40410

w¹¹¹⁸; P{GMR85A01-GAL4}attP2

40413

w¹¹¹⁸; P{GMR85A07-GAL4}attP2

40415

w¹¹¹⁸; P{GMR85A11-GAL4}attP2

47109

w¹¹¹⁸; P{GMR81H11-GAL4}attP2

47967

w¹¹¹⁸; P{GMR85A12-GAL4}attP2

48627

w¹¹¹⁸; P{GMR85A02-GAL4}attP2

600663

w¹¹¹⁸; P{R85A12-p65.AD}JK22C

54379

w¹¹¹⁸; P{GMR85A02-lexA}attP40

Korea

15545

P{GMR85B01-GAL4}attP2

15565

P{GMR85A03-GAL4}attP2

VDRC

v106875

P{KK110915}VIE-260B

v28415

w¹¹¹⁸; P{GD12818}v28415

More stocks available...

Genomic Clones (32)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (3)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP01538

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0016660

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0016660

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Leal et al., 2009)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

mid and H15 are negative regulators of wg expression and act to break the symmetry of hh signaling during segmentation of the embryo.

(Buescher et al., 2004)

Loss of both mid and H15 produces defects that mimic a wg gain-of-function phenotype.

(Buescher et al., 2004)

H15 may have a role in heart formation.

(Griffin et al., 2000)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: H15 nmr

(Qian, 2003.2.10)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

CG6604

(National Institute of Genetics Fly Stocks, 2022-, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Klebes et al., 2005)

Dm-H15

(Prpic et al., 2003)

H15

(Huang et al., 2023, Brook, 2022.9.10, Katti et al., 2022, Mallart et al., 2022, Stevens et al., 2022, Bilder et al., 2021, McLaughlin et al., 2021, Ruiz-Losada et al., 2021, Slaidina et al., 2021, Shokri et al., 2019, Svendsen et al., 2019, Wittes and Schüpbach, 2019, Schwarz et al., 2018, Liu et al., 2017, Osterfield et al., 2017, Revaitis et al., 2017, Transgenic RNAi Project members, 2017-, van Tienen et al., 2017, Fregoso Lomas et al., 2016, Moulton and Letsou, 2016, Peng et al., 2016, Gill et al., 2015, Grotewiel and Bettinger, 2015, Kumar et al., 2015, Lovato et al., 2015, Svendsen et al., 2015, Ocorr et al., 2014, Das et al., 2013, Fregoso Lomas et al., 2013, Jin et al., 2013, McKay and Lieb, 2013, Formaz-Preston et al., 2012, Johnson et al., 2011, Neumüller et al., 2011, Ryu et al., 2011, Neely et al., 2010, Gaziova and Bhat, 2009, Leal et al., 2009, Svendsen et al., 2009, Estella and Mann, 2008, Engström et al., 2007, Estella and Mann, 2007, Monier et al., 2007, Schwartz and Pirrotta, 2007, Buescher et al., 2006, Choksi et al., 2006, Joshi et al., 2006, Nguyen and Frasch, 2006, Zaffran et al., 2006, Copley, 2005, Hoskins et al., 2005, Ringrose et al., 2003)

Nmr

(Mann et al., 2007)

Nmr-1

(Lacin et al., 2014, Babaoglan et al., 2009)

Nmr1

(Ocorr et al., 2014, Tang et al., 2013)

Tbx20

(Qian et al., 2008)

dm-H15

(Papaioannou and Silver, 1998)

nmr

(Kumar et al., 2015, Qian et al., 2008, Qian, 2003.2.10, Qian et al., 2003, Cripps and Olson, 2002, Griffin et al., 2000, McGhee, 2000, Porsch et al., 1998)

nmr1

(Leal et al., 2009, Qian and Bodmer, 2009, Qian et al., 2008, Qian et al., 2005, Qian et al., 2005, Qian, 2004.2.25)

nmr^H15

(Taghli-Lamallem et al., 2008)

Name Synonyms

H15

(Osterfield et al., 2017)

Neuromancer

(Zhu et al., 2017, Mann et al., 2007)

neuromancer

(Ugur et al., 2016, Qian et al., 2008, Qian et al., 2003)

neuromancer 1

(Svendsen et al., 2009, Qian et al., 2008, Qian et al., 2005)

neuromancer 2

(Ryu et al., 2011)

neuromancer-1

(Neely et al., 2010)

neuromancer1

(Leal et al., 2009, Qian, 2004.2.25, Qian et al., 2004)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33769

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q94890

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q94890

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2J9F2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q94890

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016660

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33769/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016660

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016660

FlyMine - An integrated database for Drosophila genomics

FBgn0016660

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6604

MARRVEL_MODEL - MARRVEL (model organism gene)

33769

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0016660

DroID - A comprehensive database of gene and protein interactions.

FBgn0016660

DRSC - Results frm RNAi screens

FBgn0016660

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

H15_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016660

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0016660

Flygut - An atlas of the Drosophila adult midgut

FBgn0016660

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

33769

MIST (protein-protein) - An integrated Molecular Interaction Database

33769

References (114)

The following external sites may use different assemblies or annotations than FlyBase.

-0.52

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