endopeptidase involved in the extra-cellular metabolism of signalling peptides - metallopeptidase - regulates insulin-like peptide (ILP) expression and food intake - required in the mushroom body for middle-term and long-term memory
Please see the JBrowse view of Dmel\Nep4 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.47
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nep4 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Comment: founder cell
Comment: founder cell
Comment: lateral posterior
Comment: levels decline with age
Nep4 is first detected in embryonic stage 12 in the dorsal mesoderm in a striped pattern. From stage 13-17 it is detected in the cardiogenic mesoderm in two one-cell wide rows along the anterior-posterior axis of the embryo. Expression is observed in glial cells but not neurons in the CNS from stage 14 on. Comparisons to eve, Kr, and run reveal that Nep4 is expressed in some but not all muscle founder cells in stages 12-14. Specifically, it is expressed in the progenitor of the eve pericardial cells, muscle founder DA1, muscle founder DO2 and a third muscle founder of unknown identity. Founder cell expression is transient but expression in pericardial cells persists. In addition, Nep4 is expressed in the gonads of 50% of individuals in late embryonic stages suggesting sex specificity. It is detected in adult testes from early spermatocytes to late stage spermatids and is also detected in mature sperm. It is not found in ovaries.
The level of Nep4 declines with age in adults. It was reduced by ~20% by 25 days as compared to the level at 2 days.
Comment: founder cell
Comment: founder cell
Nep4 is first detected in embryonic stage 12 in the dorsal mesoderm in a striped pattern. From stage 13-17 it is detected in the cardiogenic mesoderm in two one-cell wide rows along the anterior-posterior axis of the embryo. Expression is observed in three major types of glial cells in the CNS from stage 14 on but not in neurons. The glial cells were identified as cell body glia, lateral glia, and the medial intersegmental nerve root glia. Comparisons to eve, Kr, and run reveal that Nep4 is expressed in some but not all muscle founder cells in stages 12-14. Specifically, it is expressed in the progenitor of the eve pericardial cells, muscle founder DA1, muscle founder DO2 and a third muscle founder of unknown identity. Founder cell expression is transient but expression in pericardial cells persists. In addition, Nep4 is expressed in the gonads of 50% of individuals in late embryonic stages suggesting sex specificity. It is detected in adult testes from early spermatocytes to late stage spermatids and is also detected in mature sperm. It is not found in ovaries.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Nep4 in JBrowse3-69
3-72.8
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Source for identity of: Nep4 CG4058