FlyBase Allele Report: Dmel\bgcn[1]

General Information

Symbol

Dmel\bgcn¹

Species

D. melanogaster

Name

FlyBase ID

FBal0001114

Feature type

allele

Associated gene

Dmel\bgcn

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Amino acid replacement: W1067term.

(Ohlstein et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,854,062..23,854,062 [-]

Nucleotide change:

G23854062A

Amino acid change:

W1067term | bgcn-PA; W1062term | bgcn-PB

Reported amino acid change:

W1067term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ohlstein et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

female sterile (with bgcn^z2-1748)

(Zinshteyn and Barbash, 2022)

female sterile | recessive

(Ohlstein et al., 2000, Lavoie et al., 1999)

male sterile | recessive

(Ohlstein et al., 2000)

viable

(Ohlstein et al., 2000)

Phenotype Manifest In

cyst cell of ovary

(Lavoie et al., 1999)

fusome

(Lavoie et al., 1999)

germarium

(Lavoie et al., 1999)

germline cell

(Ohlstein et al., 2000)

germline stem cell

gonad

(Ohlstein et al., 2000)

Detailed Description

Statement

Reference

The reduced fertility of bam^Δ59/bam^BW females is enhanced by combination with a single copy of bgcn¹ : the females are sterile with completely tumorous ovaries.

(Flores et al., 2015)

Homozygotes have germline tumours in their gonads.

(Ohlstein et al., 2000)

Germaria are undifferentiated and hyperplastic. Fusomes remain uniformly spherical rather than adopting the branched morphology characteristic of cystocytes.

(Lavoie et al., 1999)

Gametocyte differentiation defective; gonial proliferation unchecked; gonads of both males and females become engorged with gonial cells. Ovarioles appear sausage-like; testes smaller than normal.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

bam^z3-2884/bam^Δ59, bgcn[+]/bgcn¹ has female sterile phenotype, suppressible by Dsim\bam^bam.Venus

(Flores et al., 2015)

NOT suppressed by

Statement

Reference

bgcn^z2-1748/bgcn¹ has female sterile phenotype, non-suppressible by stwl^j6C3/Df(3L)Exel6122

(Zinshteyn and Barbash, 2022)

Enhancer of

Statement

Reference

bgcn[+]/bgcn¹ is an enhancer of female semi-sterile phenotype of bam^z3-2884/bam^Δ59

(Flores et al., 2015)

bgcn[+]/bgcn¹ is an enhancer of neoplasia | adult stage | female phenotype of bam^z3-2884/bam^Δ59

(Flores et al., 2015)

bgcn[+]/bgcn¹ is an enhancer of female sterile phenotype of bam^z3-2884/bam^Δ86

(Ohlstein et al., 2000)

NOT Suppressor of

Statement

Reference

bgcn^z2-1748/bgcn¹ is a non-suppressor of female sterile phenotype of stwl^j6C3/Df(3L)Exel6122

(Zinshteyn and Barbash, 2022)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

bgcn¹ has germline cell phenotype, non-suppressible by bam^hs.PO

(Lavoie et al., 1999)

Enhancer of

Statement

Reference

bgcn[+]/bgcn¹ is an enhancer of ovary phenotype of bam^z3-2884/bam^Δ59

(Flores et al., 2015)

bgcn[+]/bgcn¹ is an enhancer of phenotype of bam^z3-2884/bam^Δ86

(Ohlstein et al., 2000)

NOT Enhancer of

Statement

Reference

bgcn[+]/bgcn¹ is a non-enhancer of ovary phenotype of Dsim\bam^bam.Venus, bam^Δ86/bam^Δ59

(Flores et al., 2015)

Other

Statement

Reference

bam^z3-2884/bam^Δ86, bgcn¹ has germline cell phenotype

(Ohlstein et al., 2000)

bam^z3-2884/bam^Δ86, bgcn[+]/bgcn¹ has germline cell phenotype

(Ohlstein et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

bgcn¹/+ ; bam^z3-2884/bam^Δ86 females show germ cell hyperplasia. None of the germ cells contain the polyploid DNA complement that is diagnostic of nurse cell formation. These females are sterile.

(Ohlstein et al., 2000)

Overexpression of bam with P{hs-bam.O} in a homozygous bgcn¹ mutant produces a tumorous germ cell phenotype. Germ line stem cells are not lost in the adults of these heat shocked P{hs-bam.O} homozygous bgcn¹ mutants, though they are eliminated in their bgcn¹/+ siblings.

(Lavoie et al., 1999)

Xenogenetic Interactions

Statement

Reference

The ovarian defects observed in bam^Δ59/bam^Δ86;Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} xenogenetic rescue females cannot be exacerbated by combination with a single copy of bgcn¹. Additionally, combination with Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} suppresses the tumorous ovary phenotype characteristic for bam^BW/bam^Δ59;bgcn¹/+ double mutant flies.

(Flores et al., 2015)

Complementation and Rescue Data

Partially rescued by

bgcn¹ is partially rescued by bgcn^hs.PO

(Ohlstein et al., 2000)

Comments

Homozygous bgcn¹ females expressing bgcn^hs.PO using heat shock have maturing egg chambers and germaria organised into morphologically distinct regions 1,2 and 3 that are formed as cysts are assembled. Ovaries in older animals also have egg chambers with 15 nurse cells and one yolk-accumulating oocyte. The females are not fertile. Late stage egg chambers (stage 12 and later) contain small oocytes, misshapen follicle cell layers and are not laid. Spermatogenesis has not been rescued in homozygous bgcn¹ males expressing bgcn^hs.PO using heat shock.

(Ohlstein et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

6054

bgcn¹/CyO, P{sevRas1.V12}FK1

108568

101302

101843

102021

w¹ ; b¹ bgcn¹ / SM6a ; TM6B

Notes on Origin

Discoverer

Comments

Autonomous in transplants of young adult ovaries and testes into body cavity of wild-type females.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

bgcn¹

(Zhang et al., 2023, Berry et al., 2022, Szarka-Kovács et al., 2022, Zinshteyn and Barbash, 2022, Flores et al., 2015, Insco et al., 2012, Insco et al., 2009, Christensen et al., 2008.9.3, Christensen et al., 2008.9.3, Christensen et al., 2008.12.28, Christensen et al., 2008.12.28, Parma et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (17)

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