FlyBase Allele Report: Dmel\bam[z3-2884]

General Information

Symbol

Dmel\bam^z3-2884

Species

D. melanogaster

Name

FlyBase ID

FBal0397758

Feature type

allele

Associated gene

Dmel\bam

Associated Insertion(s)

Carried in Construct

Also Known As

bam^bw, bam^Z

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T. Amino acid replacement: L255F.

(Ohlstein et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:25,244,150..25,244,150 [-]

Nucleotide change:

C25244150T

Reported nucleotide change:

C?T

Amino acid change:

L255F | bam-PA

Reported amino acid change:

L255F

(Ohlstein et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female semi-sterile (with bam^Δ59)

(Flores et al., 2015)

female semi-sterile (with bam^Δ86)

(Ohlstein et al., 2000)

female sterile (with bam^Δ86)

(Shen et al., 2009)

male sterile

(Ohlstein et al., 2000)

neoplasia | adult stage | female (with bam^Δ59)

(Flores et al., 2015)

Phenotype Manifest In

cystoblast | increased number (with bam^Δ86)

(Pan et al., 2014)

egg chamber (with bam^Δ86)

(Shen et al., 2009)

female germline stem cell | increased number (with bam^Δ86)

(Shen et al., 2009)

germarium (with bam^Δ86)

(Shen et al., 2009, Ohlstein et al., 2000)

germline cell

(Ohlstein et al., 2000)

ovariole (with bam^Δ86)

(Shen et al., 2009)

ovary (with bam^Δ59)

(Flores et al., 2015)

ovary (with bam^Δ86)

(Ohlstein et al., 2000)

Detailed Description

Statement

Reference

bam^BW/bam^Δ59 adult females are weakly fertile with a mix of tumorous and normal egg chambers, the ovarian phenotype is significantly less severe in flies infected with Wolbachia pipientis compared to those cured of the infection and their fertility is also highly increased.

(Flores et al., 2015)

In bam^z3-2884/bam^Δ86 mutant ovaries, the germaria and most of the egg chambers are filled with undifferentiated cystoblasts.

(Pan et al., 2014)

Each bam^z3-2884/bam^Δ86 transheterozygous ovariole has a tumorous germarium with 0 or 1 normal egg chambers, in contrast to wild type ovarioles that have 7-10 egg chambers after the germarium.

In bam^z3-2884/bam^Δ86 ovarioles, the tumorous germaria and egg chambers contain many single germline stem cells.

(Shen et al., 2009)

Mutants show a tumourous gonial cell phenotype. bam^z3-2884/bam^Δ86 females are weakly fertile with small ovaries containing mostly wild-type egg chambers. Many germaria lack morphologically distinct cysts. Egg chambers containing endoreduplicating nurse cells and having a monolayer of follicle cells surrounding each egg chamber are seen.

(Ohlstein et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

bam^z3-2884/bam^Δ59 has female semi-sterile phenotype, enhanceable by bgcn[+]/bgcn¹

(Flores et al., 2015)

bam^z3-2884/bam^Δ59 has neoplasia | adult stage | female phenotype, enhanceable by bgcn[+]/bgcn¹

(Flores et al., 2015)

bam^z3-2884/bam^Δ86 has female sterile phenotype, enhanceable by bgcn[+]/bgcn¹

(Ohlstein et al., 2000)

Suppressed by

Statement

Reference

bam^z3-2884/bam^Δ59, bgcn[+]/bgcn¹ has female sterile phenotype, suppressible by Dsim\bam^bam.Venus

(Flores et al., 2015)

bam^z3-2884/bam^Δ86 has female sterile phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰⁰⁶

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female sterile phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰¹³

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female sterile phenotype, suppressible by eIF-4a[+]/eIF4A^k01501

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female sterile phenotype, suppressible by eIF-4a[+]/eIF4A⁰²⁴³⁹

(Shen et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

bam^z3-2884/bam^Δ59 has ovary phenotype, enhanceable by bgcn[+]/bgcn¹

(Flores et al., 2015)

bam^z3-2884/bam^Δ86 has cystoblast | increased number phenotype, enhanceable by CSN5^N/CSN5[+]

(Pan et al., 2014)

bam^z3-2884/bam^Δ86 has cystoblast | increased number phenotype, enhanceable by Nedd8[+]/Nedd8^AN015

(Pan et al., 2014)

bam^z3-2884/bam^Δ86 has phenotype, enhanceable by bgcn[+]/bgcn¹

(Ohlstein et al., 2000)

Suppressed by

Statement

Reference

bam^z3-2884/bam^Δ86 has cystoblast | increased number phenotype, suppressible by CSN4[+]/CSN4^N

(Pan et al., 2014)

bam^z3-2884/bam^Δ86 has cystoblast | increased number phenotype, suppressible by CSN4^k08018/CSN4[+]

(Pan et al., 2014)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰⁰⁶

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰¹³

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female germline stem cell | increased number phenotype, suppressible by eIF-4a[+]/eIF4A^k01501

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female germline stem cell | increased number phenotype, suppressible by eIF-4a[+]/eIF4A⁰²⁴³⁹

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by vas[+]/vas^RJ36

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by eIF4E1^S058911/eIF-4E[+]

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by eIF-4a[+]/eIF4A^k01501

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has ovariole phenotype, suppressible by eIF-4a[+]/eIF4A⁰²⁴³⁹

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has germarium phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰⁰⁶

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has germarium phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰¹³

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has germarium phenotype, suppressible by eIF-4a[+]/eIF4A^k01501

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has germarium phenotype, suppressible by eIF-4a[+]/eIF4A⁰²⁴³⁹

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female germline stem cell | increased number phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰⁰⁶

(Shen et al., 2009)

bam^z3-2884/bam^Δ86 has female germline stem cell | increased number phenotype, suppressible by eIF-4a[+]/eIF4A¹⁰¹³

(Shen et al., 2009)

Other

Statement

Reference

bam^z3-2884/bam^Δ86, bgcn¹ has germline cell phenotype

(Ohlstein et al., 2000)

bam^z3-2884/bam^Δ86, bgcn[+]/bgcn¹ has germline cell phenotype

(Ohlstein et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced fertility of bam^Δ59/bam^BW females is enhanced by combination with a single copy of bgcn¹ : the females are sterile with completely tumorous ovaries.

(Flores et al., 2015)

One copy of CSN4^N dramatically suppresses the increase in the number of undifferentiated cystoblasts seen in bam^z3-2884/bam^Δ86 mutants, dramatically increasing the number of differentiated cysts containing a branched fusome and the number of normal egg chambers per ovariole.

One copy of CSN4^k08018 dramatically suppresses the increase in the number of undifferentiated cystoblasts seen in bam^z3-2884/bam^Δ86 mutants, dramatically increasing the number of differentiated cysts containing a branched fusome and the number of normal egg chambers per ovariole.

One copy of CSN5^N drastically enhances the differentiation defects seen in bam^z3-2884/bam^Δ86 mutant cystoblasts.

One copy of Nedd8^AN015 drastically enhances the differentiation defects seen in bam^z3-2884/bam^Δ86 mutant cystoblasts.

(Pan et al., 2014)

Most bam^z3-2884/bam^Δ86; eIF-4a/+ ovarioles contain 4-6 normal egg chambers, while the germaria contain fewer single germ cells compared to bam^z3-2884/bam^Δ86 ovarioles.

The vas^RJ36 or eIF-4E^S058911 allele dominantly suppresses the bam^z3-2884/bam^Δ86 ovariole phenotype.

(Shen et al., 2009)

bgcn¹/+ ; bam^z3-2884/bam^Δ86 females show germ cell hyperplasia. None of the germ cells contain the polyploid DNA complement that is diagnostic of nurse cell formation. These females are sterile.

(Ohlstein et al., 2000)

Xenogenetic Interactions

Statement

Reference

Combination with Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} suppresses the tumorous ovary phenotype characteristic for bam^BW/bam^Δ59;bgcn¹/+ double mutant flies.

(Flores et al., 2015)

Complementation and Rescue Data

Fails to complement

bam^Δ86

(Ohlstein et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

bam^BW

(Bubnell et al., 2021, Ji et al., 2019, Flores et al., 2015, Chen et al., 2014)

bam^Z3-2884

(Shen et al., 2009)

bam^Z

(Pan et al., 2014, Shen et al., 2009)

bam^bw

(Wenzel and Aquadro, 2023)

bam^z3-2884

(Li et al., 2009)

Name Synonyms

Secondary FlyBase IDs

FBal0302051
FBal0118315

References (9)

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