FlyBase Allele Report: Dsim\bam[bam.Venus]

General Information

Symbol

Dsim\bam^bam.Venus

Species

D. simulans

Name

FlyBase ID

FBal0323843

Feature type

allele

Associated gene

Dsim\bam

Associated Insertion(s)

Carried in Construct

P{sim-bam-Venus}

Transgenic product class

genomic_DNA

(Flores et al., 2015)

wild_type

(Flores et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Flores et al., 2015)

wild_type

(Flores et al., 2015)

Mutagen

in vitro construct

(Flores et al., 2015)

Progenitor genotype

Carried in construct

P{sim-bam-Venus}

Cytology

Description

D.melanogaster bam regulatory sequences drive expression of D.simulans Dsim\bam tagged at the C-terminal end with Venus. The construct consists of approximately 1.7kb of sequence from upstream of the bam start codon, a genomic fragment encoding the Dsim\bam open reading frame with the inserted Venus tag, and approximately 1kb of sequence from downstream of the bam stop codon.

(Flores et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

bam

Encoded product / tool

Dsim\bam

Tagged with

Venus

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Dsim\bam^bam.Venus is a suppressor of male sterile phenotype of bam^Δ86/bam^BG

(Flores et al., 2015)

Dsim\bam^bam.Venus is a suppressor | partially of female sterile phenotype of bam^Δ86/bam^Δ59

(Flores et al., 2015)

Dsim\bam^bam.Venus is a suppressor of female sterile phenotype of bam^z3-2884/bam^Δ59, bgcn[+]/bgcn¹

(Flores et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Dsim\bam^bam.Venus, bam^Δ86/bam^Δ59 has ovary phenotype, non-enhanceable by bgcn[+]/bgcn¹

(Flores et al., 2015)

Other

Statement

Reference

Dsim\bam^bam.Venus, bam^Δ86/bam^Δ59 has ovary phenotype

(Flores et al., 2015)

Dsim\bam^bam.Venus, bam^Δ86/bam^Δ59 has female germline stem cell phenotype

(Flores et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} restores fertility of the otherwise sterile bam^BG/bam^Δ86 males (even under sperm exhaustion conditions).

Expression of Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} partially restores fertility of the otherwise sterile bam^Δ59/bam^Δ86 females. The ovaries of the rescue females show multiple morphological defects that accumulate with age (loss of germline stem cells, improper number of cyst divisions, mitotic synchrony defects). However, when two copies of the Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} transgene are used, the fertility of the rescue females is reduced (compared to one copy rescue) and they become virtually sterile by day 15. They also show accelerated loss of the germline stem cells from the ovaries and often lack them or any germline cells altogether. These effects are alleviated even by a single copy of endogenous wild-type bam. The partial fertility of bam^Δ59/bam^Δ86;Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} xenogenetic rescue flies is also improved by Wolbachia pipientis infection.

The ovarian defects observed in bam^Δ59/bam^Δ86;Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} xenogenetic rescue females cannot be exacerbated by combination with a single copy of bgcn¹. Additionally, combination with Dsim\bam^{bam.T:Avic\GFP-YFP.Venus} suppresses the tumorous ovary phenotype characteristic for bam^BW/bam^Δ59;bgcn¹/+ double mutant flies.

(Flores et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Dsim\bam^{bam.T:Avic\GFP-YFP.Venus}

Dsim\bam^bam.Venus

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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