FlyBase Allele Report: Dmel\vari[03953b]

General Information

Symbol

Dmel\vari^03953b

Species

D. melanogaster

Name

FlyBase ID

FBal0008046

Feature type

allele

Associated gene

Dmel\vari

Associated Insertion(s)

Carried in Construct

Also Known As

vari^3953b

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Beitel and Krasnow, 2000)

Mutagen

P-element activity

(Timmons et al., 1993)

Progenitor genotype

Cytology

Description

17bp deletion in the third common intron.

(Wu et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:20,791,101..20,791,117 [-]

Comment:

17bp deletion in the third common intron; flanking sequences are CTTGC_AGTTG.

(Wu et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2L)TW2)

(Wu et al., 2007)

lethal (with vari³²⁷)

(Wu et al., 2007)

lethal (with vari^f00033)

(Wu et al., 2007)

lethal (with vari^HM2043)

(Wu et al., 2007)

lethal (with vari^R3)

(Wu et al., 2007)

lethal (with vari^R979)

(Wu et al., 2007)

lethal (with vari^R2376)

(Wu et al., 2007)

lethal | recessive

(Wu et al., 2007, Timmons et al., 1993)

lethal - all die during embryonic stage (with vari^MD109)

(Bachmann et al., 2008)

partially lethal - majority live (with vari^38EFa2)

(Wu et al., 2007)

some die during embryonic stage (with vari^MD109)

(Bachmann et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Avic\GFP[Atpα-ZCL1792] and Avic\GFP[Nrx-IV-CA06597] exhibit fast recovery rates in FRAP experiments in lateral epidermal cells of vari^03953b embryos.

(Königsmann et al., 2020)

The tracheal dorsal trunk is long and tortuous in mutant embryos compared to wild type.

(Swanson et al., 2009)

vari^MD109/vari^03953b embryos die at the end of embryogenesis with convoluted tracheal tubes. Pleated septate junctions are missing between epidermal and between tracheal cells in stage 16 homozygous embryos, while adherens junctions are well developed.

(Bachmann et al., 2008)

vari^48EP/vari^03953b embryos have large dilations along the dorsal trunk.

(Moyer and Jacobs, 2008)

Trachea are longer than normal in mutant embryos.

Mutants show defects in septate junction barrier function (as tested using a dye exclusion assay). The defects show variable penetrance and expressivity depending on the genetic background.

(Wu et al., 2007)

Unlike wild-type, post stage 15 trachea in vari^03953b homozygotes are unable to exclude from their lumens, a fluorescently labelled 10kDa dextran injected into the body cavity. This is consistent with these trachea lacking a functioning septate junction barrier.

(Paul et al., 2003)

Mutation has no obvious effect on the morphology of the salivary gland or hindgut. Early tracheal development, including primary branch budding and outgrowth and lumen formation and morphology occur normally during stages 11-14. Stage 16 embryos show a number of tracheal defects. These include an increased length of the dorsal trunk compared to wild type (with no accompanying increase in cell number), mild defects in the diameter of the dorsal trunk and moderate defects in the diameter of other primary branches. Irregular and variable tracheal tube morphologies, with local constrictions and/or dilations are seen in all major branches. The defects are first seen at stage 15.

(Beitel and Krasnow, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

vari^03953b has presumptive embryonic/larval tracheal system phenotype, enhanceable by pio^2R-20

(Swanson et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The tracheal tube length defects caused by vari^03953b are enhanced in the embryos are also mutant for pio^2R-20.

(Swanson et al., 2009)

The tracheal defects of sinu^nwu7 mutant embryos are more severe in double mutant combination with vari^03953b (severe diameter defects are seen in all multicellular branches and dorsal trunk length appears increased).

(Wu et al., 2004)

The tracheal phenotypes of nrv2^k04223 homozygotes are exacerbated by vari^03953b/vari^03953b.

(Paul et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

vari^MD109

(Bachmann et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11359

P{PZ}aop^03953a, vari^03953b cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Separable from: P{PZ}aop^03953a.

(Gelbart, 1999.7.20)

Separable from: P{PZ}aop^03953a. The vari^03953b mutation is separable from the aop^03953a mutation caused by the P{PZ}aop^03953a insertion.

(Beitel and Krasnow, 2000)

Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

Lethality not caused by P{PZ}aop^03953a.

(Gelbart, 1999.7.20)

Complements: dia². Complements: dia⁵.

(Beitel and Krasnow, 2000)

Complements: l(2)00231⁰⁰²³¹. Complements: l(2)09639⁰⁹⁶³⁹. Complements: l(2)k09903^k09903. Complements: l(2)k11038^k11038. Complements: l(2)s4989^s4989. Complements: l(2)s5211^s5211. Complements: l(2)s5379^s5379.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

3953b

(Wu et al., 2007)

l(2)03953⁰³⁹⁵³

(Gelbart, 1999.7.20, BDGP Project Members, 1994-1999, Timmons et al., 1993)

l(2)03953b

(Beitel and Krasnow, 2000)

vari⁰³⁹⁵³

vari^03953b

(Königsmann et al., 2020, Hildebrandt et al., 2015, Bachmann et al., 2008, Moyer and Jacobs, 2008, Tonning et al., 2005)

vari^3953b

(Swanson et al., 2009, Paul et al., 2003)

vari^l(2)03953

(Wu et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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