FlyBase Allele Report: Dmel\N[nd-3]

General Information

Symbol

Dmel\N^nd-3

Species

D. melanogaster

Name

FlyBase ID

FBal0012892

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Also Known As

nd³, fa^nd, N^nd3, fa^no

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Hing et al., 1994, Lyman and Young, 1993)

Mutagen

X ray

(Lyman and Young, 1993)

Progenitor genotype

Cytology

Description

Insertion producing a frameshift mutation.

(Abu-Issa and Cavicchi, 1996)

Missense mutation in the intracellular domain.

(Hing et al., 1994)

Amino acid replacement: T1561S. Amino acid replacement: C105F. Mutation at position 1561 is conservative.

(Lyman and Young, 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:3,155,989..3,155,989 [+]

Nucleotide change:

G3155989T

Amino acid change:

C105F | N-PA; C105F | N-PB

Reported amino acid change:

C105F

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lyman and Young, 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Schimke immuno-osseous dysplasia

modeled by Marcal1^UAS.cBa

(Morimoto et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

N^nd-3 hemizygous adult males display nicks in the wing margin, vein deltas as well as vein thickening phenotype.

(Sachan et al., 2013)

N^nd-3 mutant flies exhibit significantly enhanced 24 hour long-term memory formation.

(Zhang et al., 2013)

N^nd-3 wings display big notches in the wing edges, enlarged veins, and prominent delta formation.

(Peralta et al., 2009)

Mutants have a wing nicking phenotype.

(Kankel et al., 2007)

Mutant flies have 98.66 +/- 1.86 thoracic microchaetae per heminotum (compared to the wild-type number of 130.35 +/- 1.54).

(Ramain et al., 2001)

N^nd-3 flies show excessive branching in the medulla. There is no significant difference in brain volume compared to wild-type flies.

(Hassan et al., 2000)

Wing vein L3 is thickened.

(Guo et al., 1999)

Hemizygotes have nicked wings distally and thickened wing veins.

(Ligoxygakis et al., 1999)

Wing veins are thickened.

(Roch et al., 1998)

Wing margin is scalloped. Wing veins are slightly thickened.

(Royet et al., 1998)

Flies show thickening of all longitudinal veins. Dorsal and ventral implants of N^nd-3 imaginal disc tissue into wild-type imaginal discs have enlarged veins which are all corrugated.

(Milan et al., 1997)

Mild wing nicking phenotype and affects the differentiation of marginal bristles.

(Hing et al., 1994)

Thickened wing veins with deltas and variably notched wing margins.

(Lyman and Young, 1993)

Individuals homozygous for Bpt¹ and N^nd-3 exhibit no Bpt¹ phenotype.

(Duus et al., 1992)

Thick wing veins with deltas, occasional apical wing nicks.

(Gorman and Girton, 1992)

The haplo-insufficient phenotype in the mesonotum is an increase in microchaetae density when N^nd-1 and N^nd-3 are in combination with N amorph alleles.

(de Celis et al., 1991)

Semilethal, adults exhibit wing notches. Wing phenotype can be enhanced in severity by vg and sca mutations.

(Rabinow and Birchler, 1990)

Pronounced eye phenotype. Does not interact with dx^ENU.

(Xu and Artavanis-Tsakonas, 1990)

Dp(2;Y)G44 males crossed to a stock carrying N^spl-1 produce sons that have straight wings or curly wings. Curly wings result from trisomy for the 44C--50B region: slightly suppress the mutant effect of N^nd-3.

(Birchler, 1988)

At 29^oC homozygotes have a weak thick wing vein phenotype with deltas at the wing vein tips. At 18^oC and 25^oC they have thick wing veins with deltas and apical notches in the wing.

(Shellenbarger and Mohler, 1975)

Lethal when heterozygous with dominant visible alleles of N, semi-lethal when heterozygous with N^Co or N^60g11.

(Welshons, 1974)

Hemizygotes and homozygotes have nicked wings and thickened wing veins. Lethal in combination with most N alleles. N^264-103/N^nd-3 females survive when raised at 22^oC but die at 29^oC. One or several temperature sensitive periods (TSPs) for lethality occur during the embryonic, larval and prepupal-pupal stages (either a single TSP for lethality begins in the embryos stage and lasts into the pupal stage or there are several discrete TSPs).

(Foster, 1973)

Wings of both sexes notched at ends of L3 and L4 veins; wing veins enlarged and δ-like at tips. Mild mutant expression often limited to wing-vein effect. Mutant expression diminished at high temperature (Shellenbarger and Mohler, 1975). Heterozygotes show extremely weak dominance. N^nd-3/N almost completely lethal; survivors are sterile and have an exaggerated Notch phenotype. N^nd-3/fa closely resembles wild type. Heterozygotes with N^fa-g and spl are complementary; with nd and N^nd-2, heterozygotes are noncomplementary with a mild mutant expression of N^nd-3-like wings. Up to 5% of N^nd-3 males from aged cultures show hyper- and hypodeveloped external genitalia (Kroeger, 1960).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^nd-3 has visible | adult stage phenotype, enhanceable by Kap-α3^D93

(Sachan et al., 2013)

N^nd-3 has visible | adult stage phenotype, enhanceable by Kap-α3^D165

(Sachan et al., 2013)

N^nd-3 has visible | adult stage phenotype, enhanceable by Kap-α3^S033513

(Sachan et al., 2013)

N^nd-3 has visible phenotype, enhanceable by klu^d00059/klu[+]

(Kankel et al., 2007)

N^nd-3 has visible phenotype, enhanceable by sca[+]/sca^d09400

(Kankel et al., 2007)

N^nd-3 has visible phenotype, enhanceable by Atg4b^P0997/Atg4b^P0997

(Thumm and Kadowaki, 2001)

Suppressed by

Statement

Reference

N^nd-3 has visible phenotype, suppressible by GPHR^c06331/CG8090[+]

(Kankel et al., 2007)

N^nd-3 has visible phenotype, suppressible by f06222^f06222/f06222[+]

(Kankel et al., 2007)

N^nd-3 has visible phenotype, suppressible by dsh^hs.PA

(Ramain et al., 2001)

N^nd-3 has visible phenotype, suppressible by pk^pk-sple-13

(Cooper and Bray, 1999)

NOT suppressed by

Statement

Reference

N^nd-3 has visible phenotype, non-suppressible by Klp64D[+]/Klp64D^k1

(Vuong et al., 2014)

N^nd-3 has visible phenotype, non-suppressible by arm¹/arm[+]

(Vuong et al., 2014)

Suppressor of

Statement

Reference

N^nd-3/N[+] is a suppressor of visible | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

Other

Statement

Reference

N^nd-3, Yif1^doc-1/Yif1[+] has lethal phenotype

(Kandasamy et al., 2021)

N^nd-3, Yif1^doc-99/Yif1[+] has lethal phenotype

(Kandasamy et al., 2021)

N^nd-3/N[+], Yif1^doc-16 has lethal phenotype

(Kandasamy et al., 2021)

N^nd-3, e(r)^p2 has lethal phenotype

(Tsubota et al., 2011)

N^nd-3, e(r)^27-1 has lethal phenotype

(Tsubota et al., 2011)

N^nd-3, e(r)^p2 has viable phenotype

(Tsubota et al., 2003)

N^nd-3, vg^s2 has visible | dominant phenotype

(Abu-Issa and Cavicchi, 1996)

N^nd-3, vg^s2 has partially lethal phenotype

(Abu-Issa and Cavicchi, 1996)

N^nd-3, vg^s2 has visible | recessive phenotype

(Abu-Issa and Cavicchi, 1996)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^nd-3 has wing margin phenotype, enhanceable by Kap-α3^D93

(Sachan et al., 2013)

N^nd-3 has wing margin phenotype, enhanceable by Kap-α3^D165

(Sachan et al., 2013)

N^nd-3 has wing margin phenotype, enhanceable by Kap-α3^S033513

(Sachan et al., 2013)

N^nd-3 has wing margin phenotype, enhanceable by Chc⁴

(Peralta et al., 2009)

N^nd-3 has wing vein phenotype, enhanceable by Chc⁴

(Peralta et al., 2009)

N^nd-3 has wing cell phenotype, enhanceable by Chc⁴

(Peralta et al., 2009)

N^nd-3 has wing phenotype, enhanceable by klu^d00059/klu[+]

(Kankel et al., 2007)

N^nd-3 has wing phenotype, enhanceable by sca[+]/sca^d09400

(Kankel et al., 2007)

N^nd-3 has wing margin phenotype, enhanceable by Atg4b^P0997/Atg4b^P0997

(Thumm and Kadowaki, 2001)

N^nd-3 has wing vein L3 phenotype, enhanceable by Psn^W11/Psn[+]

(Guo et al., 1999)

N^nd-3 has wing vein L3 phenotype, enhanceable by Psn[+]/Psn^W6

(Guo et al., 1999)

N^nd-3 has phenotype, enhanceable by bs⁰³²⁶⁷

(Roch et al., 1998)

N^nd-3 has phenotype, enhanceable by dx^S1M

(Hing et al., 1994)

N^nd-3 has phenotype, enhanceable by wg^en11

(Hing et al., 1994)

Suppressed by

Statement

Reference

N^nd-3 has wing phenotype, suppressible by GPHR^c06331/CG8090[+]

(Kankel et al., 2007)

N^nd-3 has wing phenotype, suppressible by f06222^f06222/f06222[+]

(Kankel et al., 2007)

N^nd-3 has microchaeta phenotype, suppressible by dsh^hs.PA

(Ramain et al., 2001)

N^nd-3 has wing vein phenotype, suppressible by pk^pk-sple-13

(Cooper and Bray, 1999)

N^nd-3 has wing phenotype, suppressible by pk^pk-sple-13

(Cooper and Bray, 1999)

N^nd-3 has wing phenotype, suppressible by Scer\GAL4^32B/E(spl)mβ-HLH^UAS.cdCa

(Ligoxygakis et al., 1999)

N^nd-3 has wing phenotype, suppressible by Nle[+]/Nle^k13714

(Royet et al., 1998)

N^nd-3 has wing vein phenotype, suppressible by Nle[+]/Nle^k13714

(Royet et al., 1998)

N^nd-3 has wing phenotype, suppressible by Nle[+]/Nle^Δ8

(Royet et al., 1998)

N^nd-3 has wing vein phenotype, suppressible by Nle[+]/Nle^Δ8

(Royet et al., 1998)

NOT suppressed by

Statement

Reference

N^nd-3 has wing phenotype, non-suppressible by Klp64D[+]/Klp64D^k1

(Vuong et al., 2014)

N^nd-3 has wing phenotype, non-suppressible by arm¹/arm[+]

(Vuong et al., 2014)

Enhancer of

Statement

Reference

N^nd-3 is an enhancer of wing vein phenotype of tkv¹/tkv⁸

(de Celis, 1997)

Suppressor of

Statement

Reference

N^nd-3/N[+] is a suppressor of wing vein | ectopic | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

N^nd-3/N[+] is a suppressor of wing | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

Other

Statement

Reference

N^nd-3/N[+], Psn^W11 has wing phenotype

(Guo et al., 1999)

N^nd-3/N[+], Psn^W11 has wing margin bristle phenotype

(Guo et al., 1999)

N^nd-3, Psn^W11/Psn[+] has wing phenotype

(Guo et al., 1999)

N^nd-3, Psn^W11/Psn[+] has wing margin bristle phenotype

(Guo et al., 1999)

N^nd-3, Psn[+]/Psn^W6 has wing phenotype

(Guo et al., 1999)

N^nd-3, Psn[+]/Psn^W6 has wing margin bristle phenotype

(Guo et al., 1999)

N^nd-3, vg^s2 has wing phenotype

(Abu-Issa and Cavicchi, 1996)

N^nd-3, vg^s2 has wing vein phenotype

(Abu-Issa and Cavicchi, 1996)

N^nd-3, vg^s2 has wing | ventral phenotype

(Abu-Issa and Cavicchi, 1996)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Klp64D^k1 is unable to enhance the wing notching seen in N^nd-3 heterozygotes.

One copy of arm¹ is unable to enhance the wing notching seen in N^nd-3 heterozygotes.

(Vuong et al., 2014)

The wing margin notching phenotype observed in N^nd-3 hemizygous males is exacerbated by combination with a single copy of any of the following alleles: Kap-α3^D93, Kap-α3^D165 or Kap-α3^S033513.

(Sachan et al., 2013)

Chc⁴, N^nd-3 double hemizygous mutants display an enhanced wing phenotype compared to N^nd-3 single mutant wings. Loss of a great part of the wing surface and margin to extensive notches is observed, and loss of intervein tissue to vein overgrowth.

(Peralta et al., 2009)

The number and severity of wing notching seen in N^nd-3 animals is dominantly enhanced by sca^d09400 or klu^d00059.

The N^nd-3 wing nicking phenotype is suppressed nearly to wild type by CG8090^c06331/+.

The N^nd-3 wing nicking phenotype is suppressed to wild type by f06222^f06222/+.

(Kankel et al., 2007)

The number of microchaetae in N^nd-3 flies is increased to 119.33 +/- 1.54 by expression of dsh^hs.PA using a heat pulse.

(Ramain et al., 2001)

CG6194^P0997/CG6194^P0997 enhances the loss of wing margin seen in N^nd-3/Y flies.

(Thumm and Kadowaki, 2001)

The wing phenotypes (thickened veins and wing-edge loss) of N^nd-3 flies are completely suppressed by pk^pk-sple-13.

(Cooper and Bray, 1999)

In double heterozygotes with Psn^W6 or Psn^W11, L3 is further thickened and wings are notched compared to N^nd-3 single heterozygotes.

(Guo et al., 1999)

The N^nd-3 wing phenotype is suppressed by HLHmβ^{Scer\UAS.cdCa} expressed under the control of Scer\GAL4^32B.

(Ligoxygakis et al., 1999)

The dpp loss of vein phenotype is epistatic in dpp^s4/dpp^s8 N^nd-3 double mutants, and the vein thickening characteristic of N^nd-3 is eliminated. The thickening of veins seen in tkv¹/tkv⁸ flies is strongly enhanced in combination with N^nd-3.

(de Celis, 1997)

N^nd-3/Y; vg^s2/+ flies lack the distal portion of the ventral wing blade. The wing is greatly reduced in N^nd-3/Y ; vg^s2/vg^s2 flies, and marginal structures and veins are missing. These double homozygotes have low viability.

(Abu-Issa and Cavicchi, 1996)

Synergistic interactions with dx alleles.

(Gorman and Girton, 1992)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

N^l1N-1

(Shellenbarger and Mohler, 1975)

N^fa-g

(Shellenbarger and Mohler, 1975)

N^fa-1

(Shellenbarger and Mohler, 1975)

N^Ax-tsl

(Shellenbarger and Mohler, 1975)

N^spl-1

(Shellenbarger and Mohler, 1975)

N^l1N-ts2

(Shellenbarger and Mohler, 1975)

N^l1N-ts1

(Shellenbarger and Mohler, 1975)

Fails to complement

N^nd-p

(Tsubota et al., 2003)

N^Ax-tsl

(Shellenbarger and Mohler, 1975)

N^l1N-ts2

(Shellenbarger and Mohler, 1975)

N^l1N-ts1

(Shellenbarger and Mohler, 1975)

Rescued by

N^nd-3 is rescued by N^+tCos479

(Ramos et al., 1989)

Comments

N^+tCos479 rescues the mutant phenotype. Presence of the construct confers a Confluens phenotype indicative of supernumerary copies of N.

(Ramos et al., 1989)

Complementation is at 29^oC.

(Shellenbarger and Mohler, 1975)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

2210

N^nd-3 rb¹/C(1)DX, y¹ f¹

Kyoto

106742

N^nd-3 rb¹/C(1)DX, y¹ f¹

Notes on Origin

Discoverer

Bauer.

Comments

Mapped very close to N^264-40 by genetic recombination.

(Lyman and Young, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

N^nd-3

(Kandasamy et al., 2021, Morimoto et al., 2016, Sachan et al., 2015, Sachan et al., 2013, Tsubota et al., 2011)

N^nd3

(Vuong et al., 2014, Tremmel et al., 2013, Zhang et al., 2013, Peralta et al., 2009, Hassan et al., 2000)

fa^nd

(Baonza et al., 2000, de Celis, 1998, Roch et al., 1998, de Celis, 1997, de Celis et al., 1997, de Celis and Garcia-Bellido, 1994, de Celis et al., 1993, de Celis et al., 1991)

fa^no

(Lloyd, 2000, Shellenbarger and Mohler, 1975, Foster, 1973)

nd³

(Kankel et al., 2007, Ramain et al., 2001, Wesley and Saez, 2000, Ligoxygakis et al., 1999, Wesley, 1999, Abu-Issa and Cavicchi, 1996, de Celis et al., 1996, Lyman and Young, 1993, Gorman and Girton, 1992)

nd^fa

(Royet et al., 1998)

Name Synonyms

facet-notchoid

(Eisses, 1991)

notchoid

(Hing et al., 1994)

Secondary FlyBase IDs

References (53)

Report Sections

Open Close