FlyBase Allele Report: Dmel\slbo[01310]

General Information

Symbol

Dmel\slbo⁰¹³¹⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0015687

Feature type

allele

Associated gene

Dmel\slbo

Associated Insertion(s)

P{PZ}slbo⁰¹³¹⁰

Carried in Construct

Also Known As

slbo-lacZ, slbo¹³¹⁰, PZ1310, slbo¹, slbo^PZ1310

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Murphy et al., 1995, Montell et al., 1992)

Mutagen

Delta2-3 transposase

(Montell et al., 1992)

P-element activity

(Gajewski and Schulz, 1995)

Progenitor genotype

Associated Insertion(s)

P{PZ}slbo⁰¹³¹⁰

Cytology

Description

P{PZ} insertion 223bp upstream from the start of transcription.

(Montell et al., 1992)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Gunawan et al., 2013)

female sterile | recessive

(Spradling et al., 1999, Rorth et al., 1998, Gajewski and Schulz, 1995, Montell et al., 1992, Rorth and Montell, 1992)

semi-sterile

(Liu and Montell, 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

Border cells do not migrate in slbo⁰¹³¹⁰/slbo^e7b females. Homozygous females show defects in border cell migration.

(Borghese et al., 2006)

Border cell migration is defective in slbo⁰¹³¹⁰ egg chambers.

(Schober et al., 2005)

Marker analysis shows that polarisation of the border cell cluster is disrupted in slbo⁰¹³¹⁰ flies.

(Pinheiro and Montell, 2004)

Mutant border cells migrate poorly.

(Duchek et al., 2001)

Weakly fertile allele. The border cells fail to migrate in mutant egg chambers; the border cells are present at the tip of the egg chamber at stage 10, in contrast to wild type.

(Liu and Montell, 2001)

Border cells remain at the tip of the egg chamber in homozygous stage 10 egg chambers.

(Bai et al., 2000)

Border cells fail to migrate in slbo⁰¹³¹⁰/slbo^ry8ex2d egg chambers.

(Rorth et al., 2000)

The border follicle cell cluster remains at the anterior tip in stage 9 slbo⁰¹³¹⁰ follicles. Border cell clusters occasionally penetrate between nurse cells in stage 10b or stage 11 follicles. Typically, only a few border cells slide between nurse cells while other cells of the cluster remain at the periphery of the follicle.

(Niewiadomska et al., 1999)

Border follicle cells show some migration in 3.4% of homozygous egg chambers, a level which is not sufficient for fertility.

(Rorth et al., 1998)

Defect in the initiation of border cell migration.

(Lee et al., 1996)

Homozygous females are sterile; they lay eggs but they are never fertilised. The border follicle cells fail to migrate in homozygous egg chambers. slbo⁰¹³¹⁰/+ ; Ets97D^tne-1/Df(3R)ro80b females do not lay any eggs. The ovaries of these flies contain fewer egg chambers that have developed to stage 9 and beyond compared to those of control +/+ ; slbo⁰¹³¹⁰/+ ; Ets97D^tne-1/Df(3R)ro80b flies.

(Gajewski and Schulz, 1995)

Egg chambers from slbo⁰¹³¹⁰ homozygous females show failure of border cell migration but this phenotype is not absolute because 5% of eggs laid do develop, as a result of migration of border cells that do migrate along the wild type path, albeit 5 hours later than in wild type.

(Montell et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

slbo⁰¹³¹⁰ has abnormal developmental rate phenotype, enhanceable by tj^NIG.10034R/Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

Suppressed by

Statement

Reference

slbo⁰¹³¹⁰ has female fertile phenotype, suppressible by Abl^EP3101/Scer\GAL4^slbo.2.6

(Rorth et al., 1998)

slbo⁰¹³¹⁰ has female fertile phenotype, suppressible by Hsp27^EP329/Scer\GAL4^slbo.2.6

(Rorth et al., 1998)

slbo⁰¹³¹⁰ has female fertile phenotype, suppressible by AP-2α^EP2519/Scer\GAL4^slbo.2.6

(Rorth et al., 1998)

slbo⁰¹³¹⁰ has female sterile phenotype, suppressible by btl^hs.PM

(Murphy et al., 1995)

Suppressor of

Statement

Reference

slbo⁰¹³¹⁰/slbo[+] is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Other

Statement

Reference

Ets97D^tne-1/Df(3R)ro80b, slbo⁰¹³¹⁰/slbo[+] has decreased fecundity | female phenotype

(Gajewski and Schulz, 1995)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^slbo.2.6, slbo⁰¹³¹⁰ has border follicle cell phenotype, enhanceable by tj^NIG.10034R/Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

Scer\GAL4^slbo.2.6, slbo⁰¹³¹⁰ has border follicle cell phenotype, enhanceable by tj^UAS.cGa/Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

slbo⁰¹³¹⁰ has border follicle cell phenotype, enhanceable by aop^ACT.UAS/Scer\GAL4^slbo.2.6

(Schober et al., 2005)

slbo⁰¹³¹⁰ has border follicle cell phenotype, enhanceable by aop^UAS.cRa/Scer\GAL4^slbo.2.6

(Schober et al., 2005)

Suppressed by

Statement

Reference

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by trbl^EP1119/trbl^EP1119

(Masoner et al., 2013)

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by Stat92E^EPg31485/Scer\GAL4^slbo.2.6

(Beccari et al., 2002)

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by mbc^EPg36390/Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by jing^hs.PL

(Liu and Montell, 2001)

slbo^ry7/slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by jing^hs.PL

(Liu and Montell, 2001)

slbo^ry8/slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by jing^hs.PL

(Liu and Montell, 2001)

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by Usp47^UAS.cRa/Scer\GAL4^slbo.2.6

(Rorth et al., 2000)

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by Abl^EP3101/Scer\GAL4^slbo.2.6

(Rorth et al., 1998)

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by Hsp27^EP329/Scer\GAL4^slbo.2.6

(Rorth et al., 1998)

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by AP-2α^EP2519/Scer\GAL4^slbo.2.6

(Rorth et al., 1998)

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by EP2585^EP2585/Scer\GAL4^slbo.2.6

(Rorth et al., 1998)

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by EP2585^EP2585

(Rorth et al., 1998)

slbo⁰¹³¹⁰ has border follicle cell phenotype, suppressible by btl^hs.PM

(Murphy et al., 1995)

NOT suppressed by

Statement

Reference

slbo⁰¹³¹⁰ has border follicle cell phenotype, non-suppressible by stai^UAS.cBa/Scer\GAL4^slbo.2.6

(Borghese et al., 2006)

slbo⁰¹³¹⁰/slbo^e7b has border follicle cell phenotype, non-suppressible by jing^hs.PL

(Liu and Montell, 2001)

slbo⁰¹³¹⁰ has border follicle cell phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/bnl^UASp.cRa

(Rorth, 1998)

Suppressor of

Statement

Reference

slbo⁰¹³¹⁰/slbo[+] is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Other

Statement

Reference

Scer\GAL4^slbo.2.6, Six4^UAS.Tag:HA, slbo⁰¹³¹⁰ has border follicle cell phenotype

(Borghese et al., 2006)

Ets97D^tne-1, slbo⁰¹³¹⁰ has ovary phenotype

(Gajewski and Schulz, 1995)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^slbo.2.6>tj^NIG.10034R enhances the slbo⁰¹³¹⁰ border cell cluster migration defects in the ovary.

Overexpression of Scer\GAL4^slbo.2.6>tj^Scer\UAS.cGa in slbo⁰¹³¹⁰ mutants causes a complete blockage of border cell cluster migration.

(Gunawan et al., 2013)

trbl^EP1119/trbl^EP1119 partially suppresses the border cell migration defects seen in slbo⁰¹³¹⁰/slbo⁰¹³¹⁰ females.

(Masoner et al., 2013)

Expression of stai^Scer\UAS.cBa under the control of Scer\GAL4^slbo.2.6 does not rescue the border cell migration defects seen in homozygous slbo⁰¹³¹⁰ females.

Females expressing Six4^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^slbo.2.6 in a slbo⁰¹³¹⁰/+ background show defects in border cell migration.

(Borghese et al., 2006)

Expression of aop^Scer\UAS.cRa, driven by Scer\GAL4^slbo.2.6, weakly enhances border cell migration defects in slbo⁰¹³¹⁰ egg chambers, while expression of aop^ACT.Scer\UAS (also driven by Scer\GAL4^slbo.2.6) strongly enhances this phenotype.

(Schober et al., 2005)

When Stat92E^EPg31485 (driven by Scer\GAL4^slbo.2.6) is added to slbo⁰¹³¹⁰ mutants a suppression of the border cell phenotype is seen.

(Beccari et al., 2002)

The border cells fail to migrate in the slbo⁰¹³¹⁰ jing^22F3 double mutant background, although the border cells are still present. Border cell migration is rescued in slbo^ry8/slbo⁰¹³¹⁰, slbo⁰¹³¹⁰/slbo⁰¹³¹⁰ or slbo^ry7/slbo⁰¹³¹⁰ egg chambers when jing^hs.PL is expressed using a 1 hour heat shock (and then the flies are kept at 18^oC for 20 hours to allow migration to occur). jing^hs.PL does not rescue the border cell migration defects of slbo⁰¹³¹⁰/slbo^e7b.

(Liu and Montell, 2001)

Expression of Ubp64E^Scer\UAS.cRa under the control of Scer\GAL4^slbo.2.6 significantly suppresses the slbo⁰¹³¹⁰ border cell migration phenotype.

(Rorth et al., 2000)

The sterility and abnormal border follicle cell migration phenotype of slbo⁰¹³¹⁰ females is suppressed when Abl^EP3101, Hsp27^EP329 or α-Adaptin^EP2519 is expressed under the control of Scer\GAL4^slbo.2.6. The border follicle cell migration phenotype of slbo⁰¹³¹⁰ females is suppressed by EP2585^EP2585 both in the absence of a Scer\GAL4 driver, and more strongly in the presence of Scer\GAL4^slbo.2.6.

(Rorth et al., 1998)

bnl^{Scer\UAS.P\T.cRa} does not suppress the stalled border cell phenotype of slbo⁰¹³¹⁰ females when expressed under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Rorth, 1998)

Border cell migration and fertility defects of slbo⁰¹³¹⁰ can be rescued by btl^hs.PM. Egg chambers appear healthy and no abnormalities result from the over-expression of btl.

(Murphy et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

slbo^e7b

(Montell et al., 1992)

Rescued by

slbo⁰¹³¹⁰ is rescued by slbo^hs.PM

(Liu and Montell, 2001)

Not rescued by

slbo⁰¹³¹⁰ is not rescued by slbo^LZmut.UAS/Scer\GAL4^slbo.2.6

(Rorth et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12227

P{PZ}slbo⁰¹³¹⁰ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

Comments

16 of 20 excision lines generated with P{Δ2-3}99B from slbo⁰¹³¹⁰ had restored fertility.

(Montell et al., 1992)

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

L00632

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAA28415

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Slbo^PZ1310

(Wang et al., 2010)

fs(2)01310

(Spradling et al., 1999)

slbo⁰¹³¹⁰

(Masoner et al., 2013, Christensen et al., 2009.5.6)

slbo¹³¹⁰

(Kókai et al., 2012, Borghese et al., 2006, Schober et al., 2005, Bai et al., 2000)

slbo¹

(Ogienko et al., 2020, Gunawan et al., 2013, Duchek et al., 2001, Liu and Montell, 2001)

slbo^PZ01310

(Dai et al., 2020)

slbo^PZ1310

(Miao et al., 2020)

wf(2)slbo¹

(Montell et al., 1992)

Name Synonyms

Secondary FlyBase IDs

References (30)

Report Sections

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