FB2024_03 , released June 25, 2024
Allele: Dmel\tjUAS.cGa
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General Information
Symbol
Dmel\tjUAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Gunawan
FlyBase ID
FBal0291749
Feature type
allele
Associated gene
Dmel\tj
Associated Insertion(s)
Carried in Construct
P{UAS-tj.G}
Key Links
Transgenic product class
cDNA
(Gunawan et al., 2013)
wild_type
(Gunawan et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Gunawan et al., 2013)
wild_type
(Gunawan et al., 2013)
Mutagen
in vitro construct
(Gunawan et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-tj.G}
Cytology
Description

UASt regulatory sequences drive expression of a cDNA containing the complete open reading frame and 3' untranslated region of tj.

(Gunawan et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
tj
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Clonal expression of tjScer\UAS.cGa under the control of Scer\GAL4Act5C.PU in larval terminal filament cells leads to TF cells appearing rounder than their control neighbors, with significantly increased height and decreased width as compared to control TF cells, although they do correctly intercalate and remain in the terminal filament.

      Expression of tjScer\UAS.cGa under the control of Scer\GAL4tj-NP1624 does not cause obvious defects in the female germline stem cell niche.

      (Panchal et al., 2017)

      Overexpression of tjScer\UAS.cGa under the control of Scer\GAL4slbo.2.6 impairs border cell (BCC) cluster migration in the ovary. At mid or late stage 9, when most control BCCs are undergoing migration, almost all tjScer\UAS.cGa-overexpressing BCCs fail to migrate. Several BCCs appear to move away from the anterior tip of the follicle but are unable to detach from the epithelium. Even at stage 10b, very few BCCs reach the oocyte.

      In mosaic BCCs, tjScer\UAS.cGa-overexpressing rosette cells, unlike controls, are usually found at the trailing end of the BCC.

      (Gunawan et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Statement
      Reference

      tjUAS.cGa, Scer\GAL4NP1624 is a non-suppressor of decreased cell number | adult stage phenotype of NJF02959, Scer\GAL4NP1624

      (Panchal et al., 2017)
      Phenotype Manifest In
      Enhancer of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of tjScer\UAS.cGa fails to suppress the decrease in cap cell and germline stem cell numbers in ovaries from flies expressing NJF02959 under the control of Scer\GAL4tj-NP1624.

      (Panchal et al., 2017)

      Overexpression of Scer\GAL4slbo.2.6>tjScer\UAS.cGa in slbo01310 mutants causes a complete blockage of border cell cluster migration.

      (Gunawan et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      tjScer\UAS.cGa
      tjUAS.cGa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Gunawan
      Secondary FlyBase IDs
        References (2)