FlyBase Allele Report: Dmel\N[JF02959]

General Information

Symbol

Dmel\N^JF02959

Species

D. melanogaster

Name

FlyBase ID

FBal0239461

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02959}

Also Known As

UAS-N RNAi, UAS-Notch-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02959}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02959

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates rhabdoid cancer

modeled by Snr1^HMS00363, BacA\p35^UAS.cHa

(Xie et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with Scer\GAL4^NP1624

(Panchal et al., 2017)

Phenotype Manifest In

egg chamber | adult stage, with Scer\GAL4^NP1624

(Panchal et al., 2017)

female germline stem cell | adult stage, with Scer\GAL4^NP1624

(Panchal et al., 2017)

germarium | adult stage, with Scer\GAL4^NP1624

(Panchal et al., 2017)

germarium cap cell | adult stage, with Scer\GAL4^NP1624

(Panchal et al., 2017)

ovary | adult stage, with Scer\GAL4^NP1624

(Panchal et al., 2017)

Detailed Description

Statement

Reference

Expression of N^JF02959 under the control of Scer\GAL4^tj-NP1624 leads to fusion of follicles in the ovary, a significant reduction in the average number of cap cells per germarium, and a significant decrease in the average number of germline stem cells per germarium, but no significant change in the number of terminal filament cells. In some cases, all cap cells and germline stem cells are missing, and escort cells are misplaced to the tip of the germarium, where they make contact with the terminal filament and a differentiating germline cyst.

(Panchal et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

N^JF02959, Scer\GAL4^NP1624 has decreased cell number | adult stage phenotype, non-suppressible by tj^UAS.cGa, Scer\GAL4^NP1624

(Panchal et al., 2017)

Suppressor of

Statement

Reference

N^JF02959, Scer\GAL4^Act.PU is a suppressor of neoplasia | somatic clone | third instar larval stage phenotype of BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363

(Xie et al., 2017)

Other

Statement

Reference

N^JF02959, Scer\GAL4^NP1624, tj^NIG.10034R has abnormal size | adult stage phenotype

(Panchal et al., 2017)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

N^JF02959, Scer\GAL4^NP1624 has female germline stem cell | adult stage phenotype, non-suppressible by tj^UAS.cGa, Scer\GAL4^NP1624

(Panchal et al., 2017)

N^JF02959, Scer\GAL4^NP1624 has germarium cap cell | adult stage phenotype, non-suppressible by tj^UAS.cGa, Scer\GAL4^NP1624

(Panchal et al., 2017)

Suppressor of

Statement

Reference

N^JF02959, Scer\GAL4^Act.PU is a suppressor of wing disc | somatic clone | third instar larval stage phenotype of BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363

(Xie et al., 2017)

Other

Statement

Reference

N^JF02959, Scer\GAL4^NP1624, tj^NIG.10034R has ovary | adult stage phenotype

(Panchal et al., 2017)

N^JF02959, Scer\GAL4^NP1624, tj^NIG.10034R has terminal filament | adult stage phenotype

(Panchal et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of tj^Scer\UAS.cGa fails to suppress the decrease in cap cell and germline stem cell numbers in ovaries from flies expressing N^JF02959 under the control of Scer\GAL4^tj-NP1624.

Terminal filaments in ovaries from flies expressing tj^NIG.10034R and N^JF02959 under the control of Scer\GAL4^tj-NP1624 are considerably shorter than those from flies expressing only tj^NIG.10034R, although on average are still significantly longer than those in controls. Ovaries from flies expressing tj^NIG.10034R and N^JF02959 have a significant decrease in the number of terminal filaments, and some terminal filaments are unusually short with fewer than 7 cells.

(Panchal et al., 2017)

Xenogenetic Interactions

Statement

Reference

The aggressive tissue overgrowth observed in third instar larval wing discs carrying somatic (flip-out) clones simultaneously expressing Snr1^HMS00363 and BacA\p35^Scer\UAS.cHa driven by Scer\GAL4^Act.PU is significantly suppressed by co-expression of N^JF02959.

(Xie et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27988

y¹ v¹; P{TRiP.JF02959}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

N^JF02959

N^RNAi2

(Blanco-Obregon et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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