FB2024_03 , released June 25, 2024
Allele: Dmel\Abd-BUAS.cCa
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General Information
Symbol
Dmel\Abd-BUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Castelli-Gair
FlyBase ID
FBal0038086
Feature type
allele
Associated gene
Dmel\Abd-B
Associated Insertion(s)
Carried in Construct
P{UAS-Abd-B.m.C}
Also Known As
UAS-Abd-B, UAS-Abd-Bm, UAS-AbdBm, UAS-AbdB
Key Links
Transgenic product class
cDNA
(Castelli-Gair et al., 1994)
wild_type
(Castelli-Gair et al., 1994)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Castelli-Gair et al., 1994)
wild_type
(Castelli-Gair et al., 1994)
Mutagen
in vitro construct
(Castelli-Gair et al., 1994)
Progenitor genotype
Carried in construct
P{UAS-Abd-B.m.C}
Cytology
Description

UAS regulatory sequences drive expression of a cDNA that encodes the Abd-B m variant coding region plus 475bp of 5'UTR sequence and 225bp of 3'UTR sequence.

(Castelli-Gair et al., 1994)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Abd-B
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal segment 1, with Scer\GAL4c739

      (Casares et al., 1996)

      abdominal segment 1, with Scer\GAL4C-765

      (Casares et al., 1996)

      antenna, with Scer\GAL4upd1-E132

      (Casares et al., 1996)

      arista, with Scer\GAL4dpp.blk1

      (Percival-Smith et al., 2005)

      cyst cell of testis, with Scer\GAL4C587

      (Zhang et al., 2017)

      cyst progenitor cell | spermatogenesis, with Scer\GAL4C587

      (Zhang et al., 2017)

      dMP2 neuron | embryonic stage 17, with Abd-BM5/Abd-BM2, Scer\GAL4elav-C155

      (Miguel-Aliaga and Thor, 2004)

      dMP2 neuron | embryonic stage 17, with Scer\GAL4elav-C155

      (Miguel-Aliaga and Thor, 2004)

      dMP2 neuron | embryonic stage 17, with Scer\GAL4Vap.P0201

      (Miguel-Aliaga and Thor, 2004)

      embryonic/larval posterior spiracle, with Scer\GAL469B

      (Castelli-Gair et al., 1994)

      embryonic/larval posterior spiracle, with Scer\GAL4h-1J3

      (Castelli-Gair et al., 1994)

      embryonic/larval posterior spiracle, with Scer\GAL4Kr.PM

      (Castelli-Gair et al., 1994)

      embryonic/larval spiracle | ectopic, with Scer\GAL469B

      (Lovegrove et al., 2006)

      embryonic dorsal vessel, with Scer\GAL4unspecified

      (Lovato et al., 2002)

      embryonic somatic muscle cell, with Scer\GAL4unspecified

      (Lovato et al., 2002)

      embryonic tritocerebrum, with Scer\GAL4sca-537.4

      (Sprecher et al., 2004)

      eye, with Scer\GAL4dpp.blk1

      (Plaza et al., 2001)

      fat body/gonad primordium, with Scer\GAL4twi.PG

      (Greig and Akam, 1995)

      filzkorper | ectopic, with Scer\GAL469B

      (Castelli-Gair et al., 1994)

      filzkorper | ectopic, with Scer\GAL4h-1J3

      (Castelli-Gair et al., 1994)

      filzkorper | ectopic, with Scer\GAL4Kr.PM

      (Castelli-Gair et al., 1994)

      germinal proliferation center | spermatogenesis, with Scer\GAL4C587

      (Zhang et al., 2017)

      gonad | embryonic stage, with Scer\GAL4twi.PB

      (DeFalco et al., 2004)

      gonadal sheath proper primordium, with Scer\GAL4twi.PB

      (DeFalco et al., 2004)

      larval MP1 neuron | embryonic stage 17, with Abd-BM5/Abd-BM2, Scer\GAL4elav-C155

      (Miguel-Aliaga and Thor, 2004)

      male germline stem cell | spermatogenesis, with Scer\GAL4C587

      (Zhang et al., 2017)

      pretarsus | ectopic, with Scer\GAL4dpp.blk1

      (Percival-Smith et al., 2005)

      primary gonial cell | spermatogenesis, with Scer\GAL4C587

      (Zhang et al., 2017)

      scutellum, with Scer\GAL4C-765

      (Casares et al., 1996)

      scutum, with Scer\GAL4C-765

      (Casares et al., 1996)

      scutum & macrochaeta, with Scer\GAL4C-765

      (Casares et al., 1996)

      spiracular hair | ectopic, with Scer\GAL469B

      (Castelli-Gair et al., 1994)

      spiracular hair | ectopic, with Scer\GAL4h-1J3

      (Castelli-Gair et al., 1994)

      spiracular hair | ectopic, with Scer\GAL4Kr.PM

      (Castelli-Gair et al., 1994)

      thoracic neuroblast | larval stage, with Scer\GAL4insc-Mz1407

      (Ghosh et al., 2019)

      wing, with Scer\GAL4Bx-MS1096

      (Casares et al., 1996)

      wing, with Scer\GAL4C-765

      (Casares et al., 1996)

      wing, with Scer\GAL4rn.PU

      (Percival-Smith et al., 2013)

      wing margin, with Scer\GAL4sd-SG29.1

      (Adachi-Yamada et al., 2005)

      wing vein, with Scer\GAL4sd-SG29.1

      (Adachi-Yamada et al., 2005)
      Detailed Description
      Statement
      Reference

      Expression of Abd-BUAS.cCa under the control of Scer\GAL4insc-Mz1407 from mid third instar stage, in combination with a Gal80[ts] transgene, results in a reduced number of neuroblasts in the thoracic segments of the late third instar (male or female) larval ventral nerve cord, compared to controls.

      (Ghosh et al., 2019)

      The adulthood-only expression of Abd-BUAS.cCa (using Gal80[ts]) driven by Scer\GAL4C587, but not by Scer\GAL4VP16.nos.UTR or Scer\GAL4hh-Gal4, leads to adult testes exhibiting a significant increase in the number of cyst stem cells (and their localization away from the hub), a reciprocal decrease in the number of differentiated cyst cells, an increase in the number of early stage germline cells, and a strong increase in the number of DNA replicating cells at the apical testis tip (even away from the hub), as compared to controls. This Scer\GAL4C587-dependent expression also leads to a cell non-autonomous induction of both somatic (Zfh1) and germline (Vasa) lineage markers in some somatic-derived cells, as compared to controls.

      (Zhang et al., 2017)

      Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4rn.PU suppresses wing formation.

      (Percival-Smith et al., 2013)

      Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL469B in the trunk segments results in the formation of ectopic spiracles.

      (Lovegrove et al., 2006)

      When Abd-BScer\UAS.cCa is driven by Scer\GAL4sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.

      (Adachi-Yamada et al., 2005)

      Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 results in transformation of the arista to tarsus.

      (Percival-Smith et al., 2005)

      Embryos expressing Abd-BScer\UAS.cCa under the control of Scer\GAL4twi.PB form ectopic clusters of male-specific somatic gonadal precursor (msSGP) cells in more anterior regions of the embryo than normal. Gonads do not form in these embryos and germ cells are either scattered or associated with msSGPs.

      (DeFalco et al., 2004)

      79% of anterior dMP2 neurons survive in late stage embryos expressing Abd-BScer\UAS.cCa under the control of Scer\GAL4Vap.P0201 (in contrast to wild-type embryos, where these neurons are lost by the late embryonic stage). 100% of anterior dMP2 neurons survive in late stage embryos expressing Abd-BScer\UAS.cCa under the control of Scer\GAL4elav-C155. Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4elav-C155 in late stage Abd-BM2/Abd-BM5 embryos results in the suppression of cell death normally seen in anterior dMP2 and MP1 neurons in wild-type late stage embryos.

      (Miguel-Aliaga and Thor, 2004)

      Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 95%.

      (Sprecher et al., 2004)

      Expression of Abd-BScer\UAS.cCa in the mesoderm under the control of Scer\GAL4unspecified results in severe defects in muscle development; the skeletal muscle pattern is massively deranged and there appear to be no dorsal vessel cells (based upon staining for Mhc expression).

      (Lovato et al., 2002)

      Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.

      (Hirth et al., 2001)

      Expression of Abd-BScer\UAS.cCa (driven by Scer\GAL4dpp.blk1) results in inhibition of eye development by inducing apoptosis.

      (Plaza et al., 2001)

      Scer\GAL469B-mediated expression in wild type causes a pair of posterior spiracles to develop in all trunk segments.

      (Castelli-Gair, 1998)

      Scer\GAL4E132 induced expression in the eye-antennal disc causes transformation of the antenna into leg. Scer\GAL4Bx-MS1096 or Scer\GAL4C-765 induced expression in the wing disc causes transformation of the wing into haltere-like tissue. Scer\GAL4C-765 induced expression also causes malformed notum, fused scutellum and disorganised pattern of bristles. Scer\GAL4c739 and Scer\GAL4C-765 induced expression in the abdomen transforms A1 into a posterior abdominal segment.

      (Casares et al., 1996)

      Scer\GAL4twi.PG induced expression blocks the formation of the gonad primordium.

      (Greig and Akam, 1995)

      When driven by Scer\GAL4Kr.PM, Scer\GAL4h-1J3 or Scer\GAL469B, in a wild type background, Abd-BScer\UAS.cCa generates ectopic primordia for the posterior spiracle. These go on to develop filzkorper and spiracular hairs. Keilin's organs are suppressed in the Abd-BScer\UAS.cCa, Scer\GAL4h-1J3 or Abd-BScer\UAS.cCa, Scer\GAL4Kr.PM combination.

      (Castelli-Gair et al., 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Addition of Abd-BScer\UAS.cCa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

      (Sánchez-Higueras et al., 2014)

      Co-expression of pbScer\UAS.T:Avic\GFP-EGFP suppresses the arista-to-tarsus transformation seen in flies expressing Abd-BScer\UAS.cCa under the control of Scer\GAL4dpp.blk1.

      (Percival-Smith et al., 2005)

      Embryos coexpressing abd-AScer\UAS.cGa and Abd-BScer\UAS.cCa under the control of Scer\GAL4twi.PB have ectopic male-specific somatic gonadal precursor (msSGP) cells and ectopic somatic gonadal precursor (SGP) cells. A large group of SGPs and msSGPs associates with most of the germ cells in the region where the gonad would normally be coalescing and ectopic SGPs and msSGPs also associate with one another in more anterior regions.

      (DeFalco et al., 2004)

      Expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4lab.PH does not rescue the tritocerebral defects seen in lab14 embryonic brains.

      (Hirth et al., 2001)

      Scer\GAL469B-mediated expression of Abd-BScer\UAS.cCa in linG1 mutants does not cause ectopic posterior spiracles development in the trunk segments.

      (Castelli-Gair, 1998)

      Ubxabx-1 Ubxbx-3 Ubxpbx-1/Ubxlac1 combination transforms the metathorax into mesothorax (including transformation of halteres into wings), Scer\GAL4Bx-MS1096 induced expression allows partial rescue.

      (Casares et al., 1996)

      Scer\GAL4twi.PG induced expression of Abd-BScer\UAS.cCa blocks formation of the gonadal primordium, coexpression of Abd-BScer\UAS.cCa and abd-AScer\UAS.cGa restores gonadal mesoderm.

      (Greig and Akam, 1995)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Abd-BUAS.cCa/Scer\GAL4Abd-B-LDN rescues Abd-BM1/Abd-BLDN

      (de Navas et al., 2006)

      Abd-BUAS.cCa/Scer\GAL4Vap.P0201 rescues Abd-BM5/Abd-BM2

      (Miguel-Aliaga and Thor, 2004)
      Comments

      The loss of posterior dMP2 neurons that is seen in late stage Abd-BM2/Abd-BM5 embryos is rescued by expression of Abd-BScer\UAS.cCa under the control of Scer\GAL4Vap.P0201.

      (Miguel-Aliaga and Thor, 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Abd-BScer\UAS.cCa
      Abd-BUAS.cCa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Castelli-Gair
      (Castelli-Gair et al., 1994)
      Secondary FlyBase IDs
        References (47)