FlyBase Allele Report: Scer\GAL4[Abd-B-LDN]

General Information

Symbol

Scer\GAL4^Abd-B-LDN

Species

S. cerevisiae

Name

FlyBase ID

FBal0191375

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}Abd-B^LDN

Carried in Construct

Also Known As

AbdB-Gal4, Abd-B-Gal4, Abd-B-Gal4^LDN, Abd-B^LDN-Gal4, AbdB-GAL4^LDN, A8-Gal4

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

P-element activity

(Sanchez-Herrero, 2006.5.17)

gene conversion

(Sanchez-Herrero, 2006.5.17)

Progenitor genotype

Ecol\lacZ^Abd-B-lac1

(Foronda et al., 2006, Sanchez-Herrero, 2006.5.17)

Associated Insertion(s)

P{GawB}Abd-B^LDN

Cytology

Description

P-element swapping has allowed Ecol\lacZ^Abd-B-lac1 to swap with Scer\GAL4^Abd-B-LDN. Both are inserted at position 48,957 of the bithorax complex (coordinates according to FBrf0083285).

(Sanchez-Herrero, 2006.5.17)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15

genital disc | restricted

Comment: reflects Abd-B-RB transcript.

(Foronda et al., 2006)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

parasegment 13

(Bloomington Drosophila Stock Center, 2017)

embryonic stage 13 -- 15

parasegment 13

(Forster et al., 2010)

tracheal metamere 10

(Forster et al., 2010)

embryonic stage 15

abdominal segment 8 of genital disc

(Bloomington Drosophila Stock Center, 2017)

third instar larval stage | male

larval abdominal segment 8 of male genital disc

(Petzoldt et al., 2012, de Navas et al., 2006)

third instar larval stage | female

larval abdominal segment 8 of female genital disc

(de Navas et al., 2006)

early third instar larval stage

embryonic/larval fat body

(Banreti et al., 2014)

pupal stage P6 -- P7

abdominal segment 8 anterior compartment of male genital disc

(Suzanne et al., 2010)

pharate adult stage | female

spermatheca

(Foronda et al., 2006)

uterus

(Foronda et al., 2006)

hypogynium

(Foronda et al., 2006)

adult stage

adult fruitless vAB3 (male) neuron

(Clowney et al., 2015)

adult fruitless aDT1 neuron

(Clowney et al., 2015)

adult stage | female

hypogynial tooth

(de Navas et al., 2006)

adult posterior abdominal ganglion neuron

(Bussell et al., 2014)

Additional Information

Statement

Reference

Drives expression in abdominal segment 8 (parasegment 13) at all stages; expression is observed in parts of the male and female reproductive systems corresponding to abdominal segment 8.

(FlyBase Curators, 2017)

ScerGAL4^Abd-B-LDN drives expression in 283 +/- 10 cells in the adult female abdominal ganglion; these cells are colabeled with Abd-B protein antibodies. ScerGAL4^Abd-B-LDN also drives expression in neurons innervating oviducts, uterus, muscles near the female gonopod (vaginal plates), and the gonopod bristles.

(Bussell et al., 2014)

ScerGAL4^Abd-B-LDN drives expression in the region of the male genital disc corresponding to abdominal segment 8.

(Petzoldt et al., 2012)

ScerGAL4^Abd-B-LDN drives embryonic expression in parasegment 13, including tracheal metamere 10.

(Forster et al., 2010)

ScerGAL4^Abd-B-LDN marks the ring comprising the anterior compartment of abdominal segment 8 in the male genital disc during pupal development.

(Suzanne et al., 2010)

ScerGAL4^Abd-B-LDN drives expression in the female reproductive system at late pupal and pharate adult stages in the spermathecum, uterus, and female gonopod (vaginal plates), but not in the ovaries, female accessory glands, or oviducts.

(Foronda et al., 2006)

ScerGAL4^Abd-B-LDN drives expression in the region of the male and female genital discs corresponding to abdominal segment 8.

(de Navas et al., 2006)

ScerGAL4^Abd-B-LDN expression reflects the distribution of the Abd-B-RB (Abd-B m) transcript. At embryonic stage 15, ScerGAL4^Abd-B-LDN expression is localized to two lateral cells in each of the two lateral anterior clusters of 6-7 hdc-expressing cells of the genital disc.

(Foronda et al., 2006)

Marker for

Reflects expression of

Abd-B

(Clowney et al., 2015, Banreti et al., 2014, Bussell et al., 2014, Petzoldt et al., 2012, Forster et al., 2010, Suzanne et al., 2010, de Navas et al., 2006, Foronda et al., 2006)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | cell autonomous | embryonic stage 17, with Blimp-1^UAS.cOa

(Öztürk-Çolak et al., 2016)

abnormal mating behavior, with Abd-B^GD4680, Ecol\lexA^p65.R57C10.P, Scer\FLP1^lexAop.cSa, Scer\GAL80^αTub84B.PG

(Bussell et al., 2014)

visible, with puc^UAS.cMa

(Rousset et al., 2010)

Phenotype Manifest In

abdominal segment 8 anterior compartment | pupal stage, with sdk^HMS00292

(Uechi and Kuranaga, 2019)

abdominal segment 8 anterior compartment | pupal stage, with shg^UAS.sgGFP

(Uechi and Kuranaga, 2019)

abdominal segment 8 anterior compartment | pupal stage, with sqh^JF01103

(Uechi and Kuranaga, 2019)

adherens junction | pupal stage, with sdk^HMS00292

(Uechi and Kuranaga, 2019)

adherens junction | pupal stage, with shg^UAS.sgGFP

(Uechi and Kuranaga, 2019)

adherens junction | pupal stage, with sqh^JF01103

(Uechi and Kuranaga, 2019)

embryonic/larval tracheal dorsal trunk | cell autonomous | embryonic stage 17, with Blimp-1^UAS.cOa

(Öztürk-Çolak et al., 2016)

embryonic/larval tracheal dorsal trunk | embryonic stage, with kkv^GD2807

(Öztürk-Çolak et al., 2016)

embryonic/larval tracheocyte | cell autonomous | embryonic stage 17, with Blimp-1^UAS.cOa

(Öztürk-Çolak et al., 2016)

epithelium | pupal stage, with sdk^HMS00292

(Uechi and Kuranaga, 2019)

epithelium | pupal stage, with shg^UAS.sgGFP

(Uechi and Kuranaga, 2019)

epithelium | pupal stage, with sqh^JF01103

(Uechi and Kuranaga, 2019)

female genitalia, with Abd-B^GD4680

(Bussell et al., 2014)

genital disc, with hep^UAS.cBa

(Rousset et al., 2010)

male genitalia, with arm^GD1372

(Petzoldt et al., 2012)

male genitalia, with BacA\p35^UAS.cHa

(Suzanne et al., 2010)

male genitalia, with BacA\p35^UAS.cHa, Scer\GAL4^hh-Gal4

(Suzanne et al., 2010)

male genitalia, with Delta::N^ΔECN.UAS

(Lee et al., 2011)

male genitalia, with didum^GD1848

(Petzoldt et al., 2012)

male genitalia, with jar^GD4044

(Petzoldt et al., 2012)

male genitalia, with Myo28B1^GD4043

(Petzoldt et al., 2012)

male genitalia, with Myo31DF^RNAi.Sym.UAS

(Petzoldt et al., 2012, Suzanne et al., 2010, Speder et al., 2006)

male genitalia, with Myo61F^UAS.cHa

(Petzoldt et al., 2012)

male genitalia, with Myo95E^GD10176

(Petzoldt et al., 2012)

male genitalia, with N^UAS.cSb

(Lee et al., 2011)

male genitalia, with Rab5^GD10492

(Lee et al., 2011)

male genitalia, with sdk^HMS00292

(Uechi and Kuranaga, 2019)

male genitalia, with shg^KK103334

(Petzoldt et al., 2012)

male genitalia, with shg^UAS.cUa

(Petzoldt et al., 2012)

male genitalia, with Uvrag^NIG.6116R

(Lee et al., 2011)

male genitalia, with Vps25^GD8432

(Lee et al., 2011)

male genitalia, with zip^GD1566

(Petzoldt et al., 2012)

male genitalia, with α-Cat^GD8808

(Petzoldt et al., 2012)

male terminalia, with puc^UAS.cMa

(Rousset et al., 2010)

taenidial fold | cell autonomous | embryonic stage 17, with Blimp-1^UAS.cOa

(Öztürk-Çolak et al., 2016)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Abd-B-LDN, sdk^HMS00292 has male genitalia phenotype, enhanceable by shg[+]/shg^GFP

(Uechi and Kuranaga, 2019)

Enhancer of

Statement

Reference

Scer\GAL4^Abd-B-LDN is an enhancer of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21

(Lee et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^Abd-B-LDN/N^RNAi.P.UAS is a suppressor | partially of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21

(Lee et al., 2011)

mam^N.UAS/Scer\GAL4^Abd-B-LDN is a suppressor | partially of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21

(Lee et al., 2011)

NOT Suppressor of

Statement

Reference

Egfr^GD1654/Scer\GAL4^Abd-B-LDN is a non-suppressor of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21

(Lee et al., 2011)

Pvr^RNAi.UAS.cRa/Scer\GAL4^Abd-B-LDN is a non-suppressor of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21

(Lee et al., 2011)

ptc^NIG.2411R/Scer\GAL4^Abd-B-LDN is a non-suppressor of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21

(Lee et al., 2011)

N^ECN.UAS/Scer\GAL4^Abd-B-LDN is a non-suppressor of male genitalia phenotype of Uvrag^KG04163/Uvrag^B21

(Lee et al., 2011)

abd-A^1dB5.UAS/Scer\GAL4^Abd-B-LDN is a non-suppressor of female genitalia phenotype of Abd-B^M1/Abd-B^LDN

(de Navas et al., 2006)

abd-A^1dB5.UAS/Scer\GAL4^Abd-B-LDN is a non-suppressor of abdominal tergite 7 phenotype of Abd-B^M1/Abd-B^LDN

(de Navas et al., 2006)

abd-A^1dB5.UAS/Scer\GAL4^Abd-B-LDN is a non-suppressor of abdominal tergite 7 | ectopic | male phenotype of Abd-B^M1/Abd-B^LDN

(de Navas et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Partially rescues

Sec24CD^UAS.EGFP/Scer\GAL4^Abd-B-LDN partially rescues Sec24CD^G200

(Forster et al., 2010)

Myo31DF^UAS.cSa/Scer\GAL4^Abd-B-LDN partially rescues Myo31DF^K1

(Speder et al., 2006)

Myo31DF^UAS.GFP/Scer\GAL4^Abd-B-LDN partially rescues Myo31DF^K1

(Speder et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

55848

y¹ w^*; P{GawB}Abd-B^LDN/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^Abd-B-LDN

Name Synonyms

Secondary FlyBase IDs

References (30)

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