UASt regulatory sequences drive expression of an inverted repeat.
In contrast to control clusters, which migrate along a straight path and each the oocyte in ~4hr, nurse cells expressing shgKK103334 under the control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 causes border cells to wander. A total of 70% of the clusters migrate around the outside of the egg chamber, and even those that migrate between nurse cells do not sustain directed, posterior movement.
Flies expressing shgKK103334 under the control of Scer\GAL4slbo.2.6 show differences in the migration behaviour of border cell clusters. First, a distinct front-back polarity is evident in wild-type clusters, which show spatially segregated protrusion and retraction with high protrusion velocities predominating at the front and high retraction velocities at the back. This spatial segregation is lost in clusters lacking guidance-receptor activities. Second, these double mutant clusters display overall slower protrusion and retraction velocities.
Expression of shgKK103334 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 accelerates age-dependent germline stem cell loss in females.
Expression of shgKK103334 under the control of Scer\GAL4Abd-B-LDN results in defects in male genital rotation, with males showing either no rotation, or close to no rotation (1-90[o]) of the genitalia.
Expression of shgKK103334 under the control of Scer\GAL4Myo31DF-NP1548 results in defects in male genital rotation, with male pharate adults showing either no rotation, or close to no rotation (1-90[o]) of the genitalia.
Depletion of shg in wing margin cells through expression of shgKK103334 under the control of Scer\GAL4wg-IS650 and Scer\GAL80ts.αTub84B results in severe disruption of cell organisation, with the wing margin cell region developing to be broader and necrotic and a wing margin structure that is flattened in the transverse section. Accordingly, the dorsal and ventral wing edges do not attach. In the adult wing with shg depleted from the wing margin cells, the alignment and growth of wing margin hairs is severely disrupted, and the ectopic tanning and shrinkage of the wing blade is found, probably due to necrotic disruption of the wing margin epithelial structure.
Scer\GAL4NP1548, armGD1372, shgKK103334 has lethal phenotype
α-CatGD8808, Scer\GAL4NP1548, shgKK103334 has lethal phenotype
Scer\GAL4NP1548, shgKK103334 has male genitalia phenotype, suppressible by Myo61FRNAi.UAS, Scer\GAL4NP1548
Scer\GAL4VP16.nanos.UTR/shgKK103334 is a suppressor of female germline stem cell | germline clone phenotype of N55e11
α-CatGD8808, Scer\GAL4Abd-B-LDN, shgKK103334 has male genitalia phenotype
Expression of shgKK103334 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 suppresses the retention of N55e11 mutant germline stem cells seen in females at two weeks after clone induction.
Co-expression of Myo61FdsRNA.Scer\UAS shifts the male genitalia rotation defects seen in flies expressing shgKK103334 under the control of Scer\GAL4Myo31DF-NP1548 towards a more wild-type phenotype, with almost half of males having partial genital rotation of between 91 and 180[o].
Males co-expressing shgKK103334 and α-CatGD8808 under the control of Scer\GAL4Abd-B-LDN have defects in genital rotation, either showing partial (77%) or no (23%) rotation.
