FB2024_03 , released June 25, 2024
Allele: Dmel\Rap1rvB3
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General Information
Symbol
Dmel\Rap1rvB3
Species
D. melanogaster
Name
FlyBase ID
FBal0042716
Feature type
allele
Associated gene
Dmel\Rap1
Associated Insertion(s)
Carried in Construct
Also Known As
Rap1rv(R)B3, rv(R)B3
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Hariharan et al., 1991)
Progenitor genotype
Rap1R-1
(Hariharan et al., 1991)
Cytology
Description

Premature stop codon in the N-terminal region.

(Asha et al., 1999)

Nucleotide substitution: C?T.

(Hariharan et al., 1991)

Amino acid replacement: Q76term.

(Hariharan et al., 1991)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:1,860,986..1,860,986 [-]
Nucleotide change:

C1860986T

Amino acid change:

Q76term | Rap1-PA; Q76term | Rap1-PB; Q76term | Rap1-PC

Reported amino acid change:

Q76term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Hariharan et al., 1991)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      RrvB3 mutant eye clones show the frequent loss of R7 photoreceptors from the ommatidia, with less frequent loss of other photoreceptors and pigment cells.

      RrvB3 mutant eye clones induced early in larval life result in large retinal scars, rich in pigment cells but almost completely lacking photoreceptors. Deeper sections of these preparations show a multitude of photoreceptors lying below the base of the retina, flattened above the outer nuclear layer of the lamina.

      RrvB3 mosaic eye clones exhibit R2, R3, R4, R5 and R8 photoreceptors at high frequency, with many fewer R1/6 photoreceptors and few very R7 photoreceptors.

      RrvB3 mutant eye clones in a minute background display extensive ommatidial differentiation, with expression of photoreceptor and lens cone cell markers but the specific absence of R7 markers.

      (Mavromatakis and Tomlinson, 2012)

      Wild type eyes. Transheterozygotes with Df(3L)R-E are late larval lethal.

      (Hariharan et al., 1991)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      R[+]/Rap1rvB3 is a suppressor of eye phenotype of sevAct.hs2sev

      (Mavromatakis and Tomlinson, 2012)

      R[+]/Rap1rvB3 is a suppressor of photoreceptor cell R7 | increased number phenotype of sevAct.hs2sev

      (Mavromatakis and Tomlinson, 2012)

      R[+]/Rap1rvB3 is a suppressor of ommatidium phenotype of sevAct.hs2sev

      (Mavromatakis and Tomlinson, 2012)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Removal of one copy of R (sevAct.hs2sev/+; RrvB3/+) results in a decrease the eye roughening and a significant reduction in the multiple R7 phenotype (from 90% of the ommatidia containing multiple R7 cells to 48%).

      (Mavromatakis and Tomlinson, 2012)

      Mutation has no effect on the rough eye phenotype caused by two insertions of P{GMR-Rho1}.

      (Hariharan et al., 1995)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Revertant.

      (Hariharan et al., 1991)
      Comments
      Comments

      Fail to produce mitotic clones in the eye, this is consistent with an essential role of R gene product in cell proliferation or cell viability.

      (Hariharan et al., 1991)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      Reported As
      Symbol Synonym
      RrvB2
      RrvB3
      Rap1rv(R)B3
      (Asha et al., 1999, Li et al., 1997)
      Rap1rvB3
      rap0
      (Mavromatakis and Tomlinson, 2012)
      raprvB3
      (Mavromatakis and Tomlinson, 2012)
      rv(R)B3
      (Hariharan et al., 1995, Hariharan et al., 1991)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0042715
      References (5)