FlyBase Allele Report: Dmel\Rop[G27]

General Information

Symbol

Dmel\Rop^G27

Species

D. melanogaster

Name

FlyBase ID

FBal0048940

Feature type

allele

Associated gene

Dmel\Rop

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Harrison et al., 1994)

Mutagen

ethyl methanesulfonate

(Harrison et al., 1994)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q311term.

(Harrison et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:4,137,884..4,137,884 [-]

Nucleotide change:

C4137884T

Amino acid change:

Q311term | Rop-PA; Q311term | Rop-PB

Reported amino acid change:

Q311term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Harrison et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^128Q.FL.UAS

(Romero et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

central nervous system

(Harrison et al., 1994)

egg | germline clone

(Ruden et al., 2000)

embryonic/first instar larval cuticle

(Harrison et al., 1994)

embryonic/larval tracheal system | embryonic stage

(Tsarouhas et al., 2023)

embryonic/larval tracheal system | embryonic stage (with Df(3L)GN24)

(Tsarouhas et al., 2023)

larval multidendritic class I neuron | embryonic stage

(Peng et al., 2015)

larval multidendritic class IV neuron | embryonic stage

(Peng et al., 2015)

larval multidendritic class IV neuron | larval stage | somatic clone

(Peng et al., 2015)

larval multidendritic class IV neuron | somatic clone | third instar larval stage

(Peng et al., 2015)

oocyte | germline clone

(Ruden et al., 2000)

tracheal branch primordium

(Harrison et al., 1994)

tracheal lumen | embryonic stage

(Tsarouhas et al., 2023)

tracheal lumen | embryonic stage (with Df(3L)GN24)

(Tsarouhas et al., 2023)

Detailed Description

Statement

Reference

Rop^G27/Rop^G27 embryos display defects in the growth of the dendritic arbor in the dorsal class I and IV dendritic arborizing (da) neurons, including reduction in outgrowth of both primary dendrites and secondary lateral branches.

Rop^G27/Rop^G27 class IV da neurons in larval somatic clones first manifest defects (dendrites thinning) in dendritic growth at 72hrs after egg laying (AEL) and the phenotype (severely reduced dendritic complexity due to loss of branching, reduction in number of dendritic intersections, varicosities or terminal dendrites fragmentation) gets progressively worse at later stages of larval life, especially in terminal dendrites that show heightened sensitivity to loss of Rop. Axon growth of Rop^G27 mutant da neurons however appears normal until late in larval life (144hrs AEL) when axons show thinning and varicosities along the terminals.

(Peng et al., 2015)

Germ-line clones of this mutant often produce miniature eggs with greatly reduced surface volumes. Membranes in mature oocytes are also not intact.

(Ruden et al., 2000)

Heterozygous larvae show a 35% decrease in the amplitude of the excitatory junctional potential in the muscle at the neuromuscular junction compared to control larvae, and a 79% reduction in miniature EJP frequency.

(Wu et al., 1998)

Embryos have little or no cuticle (epidermally derived structures are also abnormal), embryos fail to hatch into larvae. Trachea do not appear to be disrupted but they fail to fill with air in later embryos, possibly because they collapse due to insufficient cuticle deposition. Defects in the extracellular accumulation of secreted products was observed in other tissues: Malpighian tubules do not contain uric acid, the lumen of salivary glands contains severely reduced glue protein levels and the gut contents are incompletely digested due to lack of digestive enzymes. Morphology of the CNS is altered, fails to dissociate from the ventral epidermis and fails to shorten in mature embryos.

(Harrison et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rop^G27 has abnormal neuroanatomy | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by Scer\GAL4^109(2)80/shi^1.UAS

(Peng et al., 2015)

Enhancer of

Statement

Reference

Rop^G27/Rop[+] is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

Suppressor of

Statement

Reference

Rop^G27/Rop[+] is a suppressor of abnormal neurophysiology phenotype of Hsap\HTT^128Q.FL.UAS, Scer\GAL4^elav-C155

(Romero et al., 2008)

Other

Statement

Reference

Rop^G27/Rop[+], Sec5^E13 has abnormal neuroanatomy | third instar larval stage phenotype

(Peng et al., 2015)

Rop^G27/Rop[+], Sec6^Ex15 has abnormal neuroanatomy | third instar larval stage phenotype

(Peng et al., 2015)

Rop^G27, nab^e310 has viable phenotype

(Clements et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rop^G27 has larval multidendritic class IV neuron | somatic clone | third instar larval stage phenotype, suppressible | partially | somatic clone by Scer\GAL4^109(2)80/shi^1.UAS

(Peng et al., 2015)

Enhancer of

Statement

Reference

Rop^G27/Rop[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

NOT Enhancer of

Statement

Reference

Rop^G27/Rop[+] is a non-enhancer of wing phenotype of Scer\GAL4^da.G32, Septin4^UAS.cMa

(Munoz-Soriano et al., 2012)

Suppressor of

Statement

Reference

Rop^G27/Rop[+] is a suppressor of rhabdomere of eye photoreceptor cell | conditional | adult stage phenotype of Hsap\HTT^128Q.FL.UAS, Scer\GAL4^GMR.PF

(Romero et al., 2008)

Other

Statement

Reference

Rop^G27, btsz^J5-2 has tracheal lumen | embryonic stage phenotype

(Tsarouhas et al., 2023)

Rop^G27, btsz^J5-2 has embryonic/larval tracheal system | embryonic stage phenotype

(Tsarouhas et al., 2023)

Rop^G27, btsz^K13-4 has tracheal lumen | embryonic stage phenotype

(Tsarouhas et al., 2023)

Rop^G27, btsz^K13-4 has embryonic/larval tracheal system | embryonic stage phenotype

(Tsarouhas et al., 2023)

Rop^G27/Rop[+], Sec5^E13 has larval multidendritic class IV neuron | third instar larval stage phenotype

(Peng et al., 2015)

Rop^G27/Rop[+], Sec6^Ex15 has larval multidendritic class IV neuron | third instar larval stage phenotype

(Peng et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Sec5^E13/Rop^G27 transheterozygous third instar larvae show decreased number of terminal dendritic branches in the class IV dendritic arborizing neurons compared to either Sec5^E13/+ or Rop^G27/+ single heterozygotes.

Sec6^Ex15/Rop^G27 transheterozygous third instar larvae show decreased number of terminal dendritic branches in the class IV dendritic arborizing neurons compared to either Sec6^Ex15/+ or Rop^G27/+ single heterozygotes.

Expression of temperature-sensitive shi^1.Scer\UAS under the control of Scer\GAL4^109(2)80 in Rop^G27/Rop^G27 somatic clones ameliorates the loss of dendritic terminal branches displayed by Rop^G27/Rop^G27 class IV dendritic arborizing (da) neurons in third instar larvae (shifted to non-permissive temperature at 48hrs after egg laying and examined at 96hrs). Compared to controls kept under the same temperature regimen the shi^1.Scer\UAS expressing Rop^G27/Rop^G27 class IV da neurons show increased number of dendrite crossings.

(Peng et al., 2015)

Heterozygosity for Rop^G27 does not enhance the wing phenotype caused by expression of two copies of Sep4^Scer\UAS.cMa under the control of Scer\GAL4^da.G32.

(Munoz-Soriano et al., 2012)

Xenogenetic Interactions

Statement

Reference

Rop^G27/+ fully suppresses the increased excitatory junction potential (EJP) amplitude and decreased failure rate seen in Hsap\HD^{128Q.FL.Scer\UAS}, Scer\GAL4^elav-C155 animals at 0.25 mM Ca[2+].

Rop^G27/+ suppresses the reduced number of rhabdomeres per ommatidium phenotype seen in Hsap\HD^{128Q.FL.Scer\UAS}, Scer\GAL4^GMR.PF animals at 20 days.

(Romero et al., 2008)

Complementation and Rescue Data

Fails to complement

(Wu et al., 1998)

(Wu et al., 1998)

(Wu et al., 1998)

(Wu et al., 1998)

Rescued by

Rop^G27 is rescued by Scer\GAL4^109(2)80/Rop^{UAS.Tag:MYC,N-YFP}

(Peng et al., 2015)

Comments

Ectopic expression of Rop^{Scer\UAS.T:Hsap\MYC,T:Avic\GFP-NYFP} under Scer\GAL4^109(2)80 in the Rop^G27/Rop^G27 class IV da neurons in third instar larvae somatic clones fully rescues the dendritic growth defects of Rop^G27 mutant neurons.

(Peng et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4381

bw¹; Rop^G27 st¹/TM6B, Tb⁺

Kyoto

107715

bw¹; Rop^G27 st¹/TM6B, Tb⁺

Notes on Origin